(data stored in SCRATCH zone)

SWISSPROT: B8IUE3_METNO

ID   B8IUE3_METNO            Unreviewed;       590 AA.
AC   B8IUE3;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   30-AUG-2017, entry version 58.
DE   SubName: Full=Asparagine synthase family amidotransferase {ECO:0000313|EMBL:ACL55188.1};
DE            EC=6.3.5.4 {ECO:0000313|EMBL:ACL55188.1};
GN   OrderedLocusNames=Mnod_0142 {ECO:0000313|EMBL:ACL55188.1};
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265 {ECO:0000313|EMBL:ACL55188.1, ECO:0000313|Proteomes:UP000008207};
RN   [1] {ECO:0000313|EMBL:ACL55188.1, ECO:0000313|Proteomes:UP000008207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060
RC   {ECO:0000313|Proteomes:UP000008207};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Marx C.J., Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS
RT   2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; CP001349; ACL55188.1; -; Genomic_DNA.
DR   RefSeq; WP_015926901.1; NC_011894.1.
DR   ProteinModelPortal; B8IUE3; -.
DR   STRING; 460265.Mnod_0142; -.
DR   MEROPS; C44.001; -.
DR   EnsemblBacteria; ACL55188; ACL55188; Mnod_0142.
DR   KEGG; mno:Mnod_0142; -.
DR   eggNOG; ENOG4105CAQ; Bacteria.
DR   eggNOG; COG0367; LUCA.
DR   HOGENOM; HOG000027492; -.
DR   KO; K01953; -.
DR   OMA; YHWYPKL; -.
DR   OrthoDB; POG091H04J2; -.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IEA:UniProtKB-EC.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006529; P:asparagine biosynthetic process; IEA:InterPro.
DR   CDD; cd01991; Asn_Synthase_B_C; 1.
DR   CDD; cd00712; AsnB; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 3.60.20.10; -; 1.
DR   InterPro; IPR006426; Asn_synth_AEB.
DR   InterPro; IPR001962; Asn_synthase.
DR   InterPro; IPR033738; AsnB_N.
DR   InterPro; IPR017535; Asparagine_synth.
DR   InterPro; IPR017932; GATase_2_dom.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Pfam; PF00733; Asn_synthase; 1.
DR   Pfam; PF13537; GATase_7; 1.
DR   PIRSF; PIRSF001589; Asn_synthetase_glu-h; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
DR   TIGRFAMs; TIGR01536; asn_synth_AEB; 1.
DR   TIGRFAMs; TIGR03104; trio_amidotrans; 1.
DR   PROSITE; PS51278; GATASE_TYPE_2; 1.
PE   4: Predicted;
DR   PRODOM; B8IUE3.
DR   SWISS-2DPAGE; B8IUE3.
KW   Amino-acid biosynthesis {ECO:0000256|PIRSR:PIRSR001589-1};
KW   Asparagine biosynthesis {ECO:0000256|PIRSR:PIRSR001589-1};
KW   ATP-binding {ECO:0000256|PIRSR:PIRSR001589-2};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008207};
KW   Glutamine amidotransferase {ECO:0000256|PIRSR:PIRSR001589-1};
KW   Ligase {ECO:0000313|EMBL:ACL55188.1};
KW   Nucleotide-binding {ECO:0000256|PIRSR:PIRSR001589-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008207};
KW   Transferase {ECO:0000313|EMBL:ACL55188.1}.
FT   DOMAIN        2    214       Glutamine amidotransferase type-2.
FT                                {ECO:0000259|PROSITE:PS51278}.
FT   NP_BIND     369    370       ATP. {ECO:0000256|PIRSR:PIRSR001589-2}.
FT   ACT_SITE      2      2       For GATase activity. {ECO:0000256|PIRSR:
FT                                PIRSR001589-1}.
FT   BINDING     100    100       Glutamine. {ECO:0000256|PIRSR:
FT                                PIRSR001589-2}.
FT   BINDING     266    266       ATP; via carbonyl oxygen.
FT                                {ECO:0000256|PIRSR:PIRSR001589-2}.
FT   BINDING     293    293       ATP; via amide nitrogen and carbonyl
FT                                oxygen. {ECO:0000256|PIRSR:PIRSR001589-
FT                                2}.
SQ   SEQUENCE   590 AA;  65046 MW;  A0B90B9AA6629B49 CRC64;
     MCGICGEIRL DGAPPDAAAV AAMAVPLRAR GPDAGGSFAQ GGIAFGHRRL SILDLSPASQ
     QPMVDPGLGL AIVFNGCIYN FRSLREELRA KGYAFFSEGD TEVILKAYHA WGPRCVERFY
     GMFAFVLWER DSGRVVIARD RLGIKPLYYA ESGSRLRFAS SLPALLAAGD LDRSIDPAAL
     HHYMSFHAVV PAPLTILKGV RKVPPATIIT IEPDGRRRQE TYWEAVVTER PDDRHMTESD
     WCEAVLATLR KAVERRLVSD VPVGVLLSGG LDSSIVVALL AEAGQRGLKT FSIGFESVGG
     EAGDEFTYSD IIAERFATDH HRIRIDSARA LPALHGAVAA MSEPMVSHDA VGFYLLSEEV
     SKHVKVVQSG QGADEIFGGY HWYPPMMQST DAVADYARVF FDRDHAEMRE ALSPSLVDGD
     VSRAFVEAHF ARAGAPRPID KALRIDTEIM LVDDPVKRVD NMTMAWGLEA RVPFLDHDLV
     ELAGRVPADL KIRDGGKFIL KQAGRRVVPS EVIDRPKGYF PVPALKYLRG PFLDLVRDVL
     NEPVARERGL FNRAYVDRLL AEPEAHMTPL RGSKLWQVAL LEFWLQTHAI
//

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