(data stored in SCRATCH zone)

SWISSPROT: YQCG_BACSU

ID   YQCG_BACSU              Reviewed;         531 AA.
AC   P45942;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   11-DEC-2019, entry version 97.
DE   RecName: Full=Ribonuclease YqcG;
DE            EC=3.1.-.-;
DE   AltName: Full=RNase YqcG;
DE   AltName: Full=Toxin YqcG;
GN   Name=yqcG; OrderedLocusNames=BSU25860;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=7704261; DOI=10.1099/13500872-141-2-323;
RA   Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.;
RT   "Complete nucleotide sequence of a skin element excised by DNA
RT   rearrangement during sporulation in Bacillus subtilis.";
RL   Microbiology 141:323-327(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=7489895; DOI=10.1016/0378-1119(95)00636-k;
RA   Medigue C., Moszer I., Viari A., Danchin A.;
RT   "Analysis of a Bacillus subtilis genome fragment using a co-operative
RT   computer system prototype.";
RL   Gene 165:GC37-GC51(1995).
RN   [5]
RP   FUNCTION AS AN RNASE, FUNCTION AS A TOXIN, AND EXPRESSION IN E.COLI.
RX   PubMed=22200572; DOI=10.1016/j.febslet.2011.12.020;
RA   Holberger L.E., Garza-Sanchez F., Lamoureux J., Low D.A., Hayes C.S.;
RT   "A novel family of toxin/antitoxin proteins in Bacillus species.";
RL   FEBS Lett. 586:132-136(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. The
CC       C-terminus (residues 379-531) has RNase activity, and inhibits growth
CC       upon expression in E.coli. In vitro RNase activity and in vivo growth
CC       inhibition are neutralized by cognate antitoxin YqcF, but not by
CC       antitoxins specific to other toxins with the LXG domain.
CC       {ECO:0000269|PubMed:22200572}.
CC   -!- SUBUNIT: Probably interacts with cognate antitoxin YqcF but not with
CC       non-cognate antitoxins. The interaction inhibits the toxic activity of
CC       YqcF (Probable). {ECO:0000305}.
DR   EMBL; D32216; BAA06964.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12428.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14527.1; -; Genomic_DNA.
DR   PIR; F69949; F69949.
DR   RefSeq; NP_390463.1; NC_000964.3.
DR   RefSeq; WP_004399034.1; NZ_JNCM01000036.1.
DR   SMR; P45942; -.
DR   STRING; 224308.BSU25860; -.
DR   PaxDb; P45942; -.
DR   PRIDE; P45942; -.
DR   EnsemblBacteria; CAB14527; CAB14527; BSU25860.
DR   GeneID; 937792; -.
DR   KEGG; bsu:BSU25860; -.
DR   PATRIC; fig|224308.179.peg.2810; -.
DR   eggNOG; COG5444; LUCA.
DR   HOGENOM; HOG000008783; -.
DR   KO; K21491; -.
DR   OMA; WIDLIEM; -.
DR   BioCyc; BSUB:BSU25860-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR006829; LXG_dom.
DR   InterPro; IPR027797; PT-TG_dom.
DR   InterPro; IPR026835; YqcG.
DR   Pfam; PF14410; GH-E; 1.
DR   Pfam; PF04740; LXG; 1.
DR   Pfam; PF14449; PT-TG; 1.
DR   PROSITE; PS51756; LXG; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P45942.
DR   SWISS-2DPAGE; P45942.
KW   Coiled coil; Hydrolase; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..531
FT                   /note="Ribonuclease YqcG"
FT                   /id="PRO_0000049777"
FT   DOMAIN          1..235
FT                   /note="LXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01092"
FT   COILED          2..39
FT                   /evidence="ECO:0000255"
FT   COILED          139..171
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   531 AA;  59669 MW;  C9A39C032891B220 CRC64;
     MKVFEAKTLL TEAEKRAQEY KDLKSKMVKL KKAFKAVADL DDSEFSGKGA NNIKSFYEDQ
     AGIADQWIDL IEMKISFLTS IPGFLEDANL SDAYIEETFL AHELANAYTK SKSIMSEQKK
     AMKDILNDIN DILPLDLFST ETFKNELSSA EKKRKEAIEK MDEVDQNLTS EYGLSEANEQ
     MIQADYQALM NATAKGKSAS PIHYNAKAYR DSEIHKMTED VKKQSTDYIS FKDQQAEQRR
     IAKEQEELAN RPWYEKSWDA VCNFTGEVSG YYDYKRAADG VDPVTGEKLT AGQRVAAGAM
     AAAGYIPIVG WAGKLAKGGK AVYSTSKALY RADKALDVYK TPKTFHALQN SSKGLYGLAS
     ANGFSEAITG RDMFGNKVSK ERQEQSLSGA MAMLVPFGAR GINKKLNAKS SSRVSEASTN
     TSKKPKVPKT YKRPTYFRKG VRDKVWENAK DSTGSVKDPL TKQVMKKDEP WDMGHKPGYE
     FRKHQQSAME RNISRKQFLD EHNNPDHYQP ELPSSNRSHK GEDMTDDYFG D
//

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