(data stored in SCRATCH zone)

SWISSPROT: CISA_BACSU

ID   CISA_BACSU              Reviewed;         500 AA.
AC   P17867;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   11-DEC-2019, entry version 124.
DE   RecName: Full=Putative DNA recombinase;
DE   AltName: Full=Stage IV sporulation protein CA;
GN   Name=cisA; Synonyms=spoIVCA; OrderedLocusNames=BSU25770;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2105293; DOI=10.1128/jb.172.2.1092-1098.1990;
RA   Sato T., Samori Y., Kobayashi Y.;
RT   "The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a
RT   protein homologous to a site-specific recombinase.";
RL   J. Bacteriol. 172:1092-1098(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=7704261; DOI=10.1099/13500872-141-2-323;
RA   Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.;
RT   "Complete nucleotide sequence of a skin element excised by DNA
RT   rearrangement during sporulation in Bacillus subtilis.";
RL   Microbiology 141:323-327(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   SEQUENCE REVISION TO 438.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- FUNCTION: Putative site-specific recombinase having a very important
CC       role in sporulation. It probably plays a role in the recombination of
CC       SpoIIIC and SpoIVCB to form sigma K factor.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the site-specific
CC       recombinase resolvase family. {ECO:0000305}.
DR   EMBL; M29040; AAA22314.1; -; Genomic_DNA.
DR   EMBL; D32216; BAA06971.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12435.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14518.2; -; Genomic_DNA.
DR   PIR; A43656; A43656.
DR   RefSeq; NP_390454.2; NC_000964.3.
DR   RefSeq; WP_010886573.1; NZ_JNCM01000036.1.
DR   SMR; P17867; -.
DR   IntAct; P17867; 6.
DR   STRING; 224308.BSU25770; -.
DR   PaxDb; P17867; -.
DR   PRIDE; P17867; -.
DR   EnsemblBacteria; CAB14518; CAB14518; BSU25770.
DR   GeneID; 937799; -.
DR   KEGG; bsu:BSU25770; -.
DR   PATRIC; fig|224308.179.peg.2801; -.
DR   eggNOG; ENOG4107Y5K; Bacteria.
DR   eggNOG; COG1961; LUCA.
DR   HOGENOM; HOG000273662; -.
DR   InParanoid; P17867; -.
DR   KO; K06400; -.
DR   OMA; VVRQILM; -.
DR   PhylomeDB; P17867; -.
DR   BioCyc; BSUB:BSU25770-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:recombinase activity; IBA:GO_Central.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR   Gene3D; 1.10.10.2600; -; 1.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR038109; DNA_bind_recomb_sf.
DR   InterPro; IPR011109; DNA_bind_recombinase_dom.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR025827; Zn_ribbon_recom_dom.
DR   Pfam; PF07508; Recombinase; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   Pfam; PF13408; Zn_ribbon_recom; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS51737; RECOMBINASE_DNA_BIND; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
DR   PRODOM; P17867.
DR   SWISS-2DPAGE; P17867.
KW   Coiled coil; DNA integration; DNA recombination; DNA-binding;
KW   Reference proteome.
FT   CHAIN           1..500
FT                   /note="Putative DNA recombinase"
FT                   /id="PRO_0000196383"
FT   DOMAIN          1..144
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   DNA_BIND        152..288
FT                   /note="Recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01073"
FT   COILED          372..448
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        9
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   CONFLICT        438
FT                   /note="E -> R (in Ref. 1; AAA22314, 2; BAA06971 and 3;
FT                   BAA12435)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   500 AA;  57455 MW;  E925F89A4BC88F16 CRC64;
     MIAIYVRVST EEQAIKGSSI DSQIEACIKK AGTKDVLKYA DEGFSGELLE RPALNRLRED
     ASKGLISQVI CYDPDRLSRK LMNQLIIDDE LRKRNIPLIF VNGEYANSPE GQLFFAMRGA
     ISEFEKAKIK ERTSSGRLQK MKKGMIIKDS KLYGYKFVKE KRTLEILEEE AKIIRMIFNY
     FTDHKSPFFG RVNGIALHLT QMGVKTKKGA KVWHRQVVRQ ILMNSSYKGE HRQYKYDTEG
     SYVSKQAGNK SIIKIRPEEE QITVTIPAIV PAEQWDYAQE LLGQSKRKHL SISPHNYLLS
     GLVRCGKCGN TMTGKKRKSH GKDYYVYTCR KNYSGAKDRG CGKEMSENKL NRHVWGEIFK
     FITNPQKYVS FKEAEQSNHL SDELELIEKE IEKTKKGRKR LLTLISLSDD DDLDIDEIKA
     QIIELQKKQN QLTEKCNEIQ SKMKVLDDTS SSENALKRAI DYFQSIGADN LTLEDKKTIV
     NFIVKEVTIV DSDTIYIETY
//

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