(data stored in SCRATCH zone)

SWISSPROT: COMEC_BACSU

ID   COMEC_BACSU             Reviewed;         776 AA.
AC   P39695;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   04-AUG-2003, sequence version 2.
DT   11-DEC-2019, entry version 127.
DE   RecName: Full=ComE operon protein 3;
GN   Name=comEC; Synonyms=comE3; OrderedLocusNames=BSU25570;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=7968523; DOI=10.1111/j.1365-2958.1993.tb00907.x;
RA   Hahn J., Inamine G., Kozlov Y., Dubnau D.A.;
RT   "Characterization of comE, a late competence operon of Bacillus subtilis
RT   required for the binding and uptake of transforming DNA.";
RL   Mol. Microbiol. 10:99-110(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   INDUCTION.
RC   STRAIN=168 / CU741;
RX   PubMed=11948146; DOI=10.1128/jb.184.9.2344-2351.2002;
RA   Ogura M., Yamaguchi H., Kobayashi K., Ogasawara N., Fujita Y., Tanaka T.;
RT   "Whole-genome analysis of genes regulated by the Bacillus subtilis
RT   competence transcription factor ComK.";
RL   J. Bacteriol. 184:2344-2351(2002).
RN   [5]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=168;
RX   PubMed=17630974; DOI=10.1111/j.1365-2958.2007.05799.x;
RA   Kramer N., Hahn J., Dubnau D.;
RT   "Multiple interactions among the competence proteins of Bacillus
RT   subtilis.";
RL   Mol. Microbiol. 65:454-464(2007).
CC   -!- FUNCTION: The comE operon is required for the binding and uptake of
CC       transforming DNA. ComEC is required for internalization but is
CC       dispensable for DNA binding. {ECO:0000269|PubMed:7968523}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Expression activated by ComK (PubMed:11948146).
CC       {ECO:0000269|PubMed:11948146}.
CC   -!- DISRUPTION PHENOTYPE: Destabilization of ComFA (PubMed:17630974).
CC       {ECO:0000269|PubMed:17630974}.
CC   -!- SIMILARITY: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI.
CC       {ECO:0000305}.
DR   EMBL; L15202; AAC36907.1; -; Unassigned_DNA.
DR   EMBL; D84432; BAA12454.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14499.1; -; Genomic_DNA.
DR   PIR; S39865; S39865.
DR   RefSeq; NP_390435.1; NC_000964.3.
DR   RefSeq; WP_009967776.1; NZ_JNCM01000036.1.
DR   SMR; P39695; -.
DR   IntAct; P39695; 2.
DR   STRING; 224308.BSU25570; -.
DR   TCDB; 3.A.11.1.1; the bacterial competence-related dna transformation transporter (dna-t) family.
DR   PaxDb; P39695; -.
DR   PRIDE; P39695; -.
DR   EnsemblBacteria; CAB14499; CAB14499; BSU25570.
DR   GeneID; 937839; -.
DR   KEGG; bsu:BSU25570; -.
DR   PATRIC; fig|224308.179.peg.2780; -.
DR   eggNOG; ENOG4105EGY; Bacteria.
DR   eggNOG; COG0658; LUCA.
DR   eggNOG; COG2333; LUCA.
DR   HOGENOM; HOG000015493; -.
DR   InParanoid; P39695; -.
DR   KO; K02238; -.
DR   OMA; RTDKQGA; -.
DR   PhylomeDB; P39695; -.
DR   BioCyc; BSUB:BSU25570-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   CDD; cd07731; ComA-like_MBL-fold; 1.
DR   Gene3D; 3.60.15.10; -; 1.
DR   InterPro; IPR035681; ComA-like_MBL.
DR   InterPro; IPR004477; ComEC_N.
DR   InterPro; IPR004797; Competence_ComEC/Rec2.
DR   InterPro; IPR025405; DUF4131.
DR   InterPro; IPR001279; Metallo-B-lactamas.
DR   InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR   Pfam; PF03772; Competence; 1.
DR   Pfam; PF13567; DUF4131; 1.
DR   Pfam; PF00753; Lactamase_B; 1.
DR   SMART; SM00849; Lactamase_B; 1.
DR   SUPFAM; SSF56281; SSF56281; 1.
DR   TIGRFAMs; TIGR00360; ComEC_N-term; 1.
DR   TIGRFAMs; TIGR00361; ComEC_Rec2; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; P39695.
DR   SWISS-2DPAGE; P39695.
KW   Cell membrane; Competence; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..776
FT                   /note="ComE operon protein 3"
FT                   /id="PRO_0000090009"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..252
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        263..283
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        387..407
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        416..436
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        475..495
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        451
FT                   /note="V -> I (in Ref. 1; AAC36907)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        639
FT                   /note="P -> K (in Ref. 1; AAC36907)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   776 AA;  86701 MW;  90F50E1EECEEFB2A CRC64;
     MRNSRLLLPM AAASATAGIT AAAYFPAIFL FILFLLIILI KTRHAFLIIV CFFSFILFFV
     LYAVTDSQNV SSYRQGTYQF KAVIDTIPKI DGDRMSMMVE TPDKEKWAAA YRIQSAGEKE
     QLLYIEPGMS CELTGTLEEP NHATVPGAFD YNEYLYRQHI HWNYSVTSIQ NCSEPENFKY
     KVLSLRKHII SFTNSLLPPD STGIVQALTV GDRFYVEDEV LTAYQKLGVV HLLAISGLHV
     GILTAGLFYI MIRLGITREK ASILLLLFLP LYVMLTGAAP SVLRAALMSG VYLAGSLVKW
     RVRSATAICL SYIVLLLFNP YHLFEAGFQL SFAVSFSLIL SSSIFQQVKT SLGQLTIVSL
     IAQLGSLPIL LYHFHQFSII SVPMNMLMVP FYTFCILPGA VAGVLLLSLS ASFGRLFFSW
     FDLLISWINR LITNIADVDV FTIMIAHPAP VLLFLFTVTI ILLLMAIEKR SLSQLMVTGG
     ICCTVMFLLF IYPCLSSEGE VDMIDIGQGD SMFVGAPHQR GRVLIDTGGT LSYSSEPWRE
     KQHPFSLGEK VLIPFLTAKG IKQLDALILT HADQDHIGEA EILLKHHKVK RLVIPKGFVS
     EPKDEKVLQA AREEGVAIEE VKRGDVLQIK DLQFHVLSPE APDPASKNNS SLVLWMETGG
     MSWILTGDLE KEGEQEVMNV FPNIKADVLK VGHHGSKGST GEEFIQQLQP KTAIISAGKN
     NRYHHPHQKV LQLLQRHSIR VLRTDQNGTI QYRYKNRVGT FSVYPPYDTS DITETN
//

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