(data stored in SCRATCH zone)

SWISSPROT: DNAK_BACSU

ID   DNAK_BACSU              Reviewed;         611 AA.
AC   P17820; P13343;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   11-DEC-2019, entry version 148.
DE   RecName: Full=Chaperone protein DnaK;
DE   AltName: Full=HSP70;
DE   AltName: Full=Heat shock 70 kDa protein;
DE   AltName: Full=Heat shock protein 70;
GN   Name=dnaK; OrderedLocusNames=BSU25470;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / MB11;
RX   PubMed=2110662; DOI=10.1093/nar/18.8.2172;
RA   Wetzstein M., Schumann W.;
RT   "Complete nucleotide sequence of the Bacillus subtilis dnaK gene.";
RL   Nucleic Acids Res. 18:2172-2172(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / MB11;
RX   PubMed=1339421; DOI=10.1128/jb.174.10.3300-3310.1992;
RA   Wetzstein M., Voelker U., Dedio J., Loebau S., Zuber U., Schiesswohl M.,
RA   Herget C., Hecker M., Schumann W.;
RT   "Cloning, sequencing, and molecular analysis of the dnaK locus from
RT   Bacillus subtilis.";
RL   J. Bacteriol. 174:3300-3310(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-572.
RC   STRAIN=168 / MB11;
RX   PubMed=2108432; DOI=10.1093/nar/18.5.1289;
RA   Wetzstein M., Schumann W.;
RT   "Nucleotide sequence of a Bacillus subtilis gene homologous to the grpE
RT   gene of E. coli located immediately upstream of the dnaK gene.";
RL   Nucleic Acids Res. 18:1289-1289(1990).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-214.
RC   STRAIN=168 / MB24;
RX   PubMed=2510131; DOI=10.1093/nar/17.20.8373;
RA   Hearne C.M., Ellar D.J.;
RT   "Nucleotide sequence of a Bacillus subtilis gene homologous to the dnaK
RT   gene of Escherichia coli.";
RL   Nucleic Acids Res. 17:8373-8373(1989).
RN   [7]
RP   PROTEIN SEQUENCE OF 2-8.
RC   STRAIN=168 / IS58;
RX   PubMed=8012595; DOI=10.1099/00221287-140-4-741;
RA   Voelker U., Engelmann S., Maul B., Riethdorf S., Voelker A., Schmid R.,
RA   Mach H., Hecker M.;
RT   "Analysis of the induction of general stress proteins of Bacillus
RT   subtilis.";
RL   Microbiology 140:741-752(1994).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC   -!- INDUCTION: By heat shock.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
DR   EMBL; X52064; CAA36286.1; -; Genomic_DNA.
DR   EMBL; M84964; AAA22528.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12464.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14489.1; -; Genomic_DNA.
DR   EMBL; X51477; CAA35842.1; -; Genomic_DNA.
DR   EMBL; X16393; CAA34429.1; -; Genomic_DNA.
DR   PIR; S09500; S09500.
DR   RefSeq; NP_390425.1; NC_000964.3.
DR   RefSeq; WP_004398786.1; NZ_JNCM01000036.1.
DR   PDB; 2FZO; Model; -; A=1-611.
DR   PDBsum; 2FZO; -.
DR   SMR; P17820; -.
DR   IntAct; P17820; 3.
DR   MINT; P17820; -.
DR   STRING; 224308.BSU25470; -.
DR   jPOST; P17820; -.
DR   PaxDb; P17820; -.
DR   PRIDE; P17820; -.
DR   EnsemblBacteria; CAB14489; CAB14489; BSU25470.
DR   GeneID; 937849; -.
DR   KEGG; bsu:BSU25470; -.
DR   PATRIC; fig|224308.179.peg.2768; -.
DR   eggNOG; ENOG4105CFG; Bacteria.
DR   eggNOG; COG0443; LUCA.
DR   HOGENOM; HOG000228137; -.
DR   InParanoid; P17820; -.
DR   KO; K04043; -.
DR   OMA; ISIKRHM; -.
DR   PhylomeDB; P17820; -.
DR   BioCyc; BSUB:BSU25470-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR   GO; GO:0016887; F:ATPase activity; IBA:GO_Central.
DR   GO; GO:0042623; F:ATPase activity, coupled; IBA:GO_Central.
DR   GO; GO:0031072; F:heat shock protein binding; IBA:GO_Central.
DR   GO; GO:0051787; F:misfolded protein binding; IBA:GO_Central.
DR   GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR   GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR   GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR   GO; GO:0034620; P:cellular response to unfolded protein; IBA:GO_Central.
DR   GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR   GO; GO:0042026; P:protein refolding; IBA:GO_Central.
DR   GO; GO:0006986; P:response to unfolded protein; IBA:GO_Central.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P17820.
DR   SWISS-2DPAGE; P17820.
KW   3D-structure; ATP-binding; Chaperone; Direct protein sequencing;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8012595"
FT   CHAIN           2..611
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078420"
FT   MOD_RES         173
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        99
FT                   /note="S -> R (in Ref. 6; CAA34429)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        150
FT                   /note="A -> P (in Ref. 6; CAA34429)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        166
FT                   /note="V -> L (in Ref. 6; CAA34429)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   611 AA;  66002 MW;  E83CEA6E16A31593 CRC64;
     MSKVIGIDLG TTNSCVAVLE GGEPKVIANA EGNRTTPSVV AFKNGERQVG EVAKRQSITN
     PNTIMSIKRH MGTDYKVEIE GKDYTPQEVS AIILQHLKSY AESYLGETVS KAVITVPAYF
     NDAERQATKD AGKIAGLEVE RIINEPTAAA LAYGLDKTDE DQTILVYDLG GGTFDVSILE
     LGDGVFEVRS TAGDNRLGGD DFDQVIIDHL VSEFKKENGI DLSKDKMALQ RLKDAAEKAK
     KDLSGVSSTQ ISLPFITAGE AGPLHLELTL TRAKFEELSS HLVERTMGPV RQALQDAGLS
     ASEIDKVILV GGSTRIPAVQ EAIKKETGKE AHKGVNPDEV VALGAAIQGG VITGDVKDVV
     LLDVTPLSLG IETMGGVFTK LIDRNTTIPT SKSQVFSTAA DNQTAVDIHV LQGERPMSAD
     NKTLGRFQLT DIPPAPRGVP QIEVSFDIDK NGIVNVRAKD LGTGKEQNIT IKSSSGLSDE
     EIERMVKEAE ENADADAKKK EEIEVRNEAD QLVFQTEKTL KDLEGKVDEE QVKKANDAKD
     ALKAAIEKNE FEEIKAKKDE LQTIVQELSM KLYEEAAKAQ QAQGGANAEG KADDNVVDAE
     YEEVNDDQNK K
//

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