(data stored in SCRATCH zone)

SWISSPROT: SIGA_BACSU

ID   SIGA_BACSU              Reviewed;         371 AA.
AC   P06224;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   11-DEC-2019, entry version 156.
DE   RecName: Full=RNA polymerase sigma factor SigA {ECO:0000255|HAMAP-Rule:MF_00963};
DE   AltName: Full=Sigma-43;
DE   AltName: Full=Sigma-A;
GN   Name=sigA {ECO:0000255|HAMAP-Rule:MF_00963}; Synonyms=rpoD;
GN   OrderedLocusNames=BSU25200;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987246;
RA   Gitt M.A., Wang L.-F., Doi R.H.;
RT   "A strong sequence homology exists between the major RNA polymerase sigma
RT   factors of Bacillus subtilis and Escherichia coli.";
RL   J. Biol. Chem. 260:7178-7185(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3086839; DOI=10.1093/nar/14.10.4293;
RA   Wang L.F., Doi R.H.;
RT   "Nucleotide sequence and organization of Bacillus subtilis RNA polymerase
RT   major sigma (sigma 43) operon.";
RL   Nucleic Acids Res. 14:4293-4307(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   SEQUENCE REVISION TO 165.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 302-371.
RC   STRAIN=168;
RX   PubMed=2166045;
RA   von Wachenfeldt C., Hederstedt L.;
RT   "Bacillus subtilis 13-kilodalton cytochrome c-550 encoded by cccA consists
RT   of a membrane-anchor and a heme domain.";
RL   J. Biol. Chem. 265:13939-13948(1990).
RN   [7]
RP   PROTEIN SEQUENCE OF 81-90, AND CONFORMATIONAL CHANGE.
RX   PubMed=8363585; DOI=10.1042/bj2940043;
RA   Chang B.-Y., Doi R.H.;
RT   "Conformational properties of Bacillus subtilis RNA polymerase sigma A
RT   factor during transcription initiation.";
RL   Biochem. J. 294:43-47(1993).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is the primary sigma factor during
CC       exponential growth. {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00963}.
CC   -!- MISCELLANEOUS: A conformational change of sigma-A occurs during its
CC       association with the core enzyme.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00963}.
DR   EMBL; M10089; AAA22709.1; -; Genomic_DNA.
DR   EMBL; X03897; CAA27538.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12489.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14450.2; -; Genomic_DNA.
DR   EMBL; J05569; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A22626; RNBS43.
DR   RefSeq; NP_390399.2; NC_000964.3.
DR   RefSeq; WP_003226225.1; NZ_JNCM01000036.1.
DR   PDB; 5MWW; NMR; -; A=2-72.
DR   PDBsum; 5MWW; -.
DR   SMR; P06224; -.
DR   IntAct; P06224; 2.
DR   STRING; 224308.BSU25200; -.
DR   jPOST; P06224; -.
DR   PaxDb; P06224; -.
DR   PRIDE; P06224; -.
DR   EnsemblBacteria; CAB14450; CAB14450; BSU25200.
DR   GeneID; 937897; -.
DR   KEGG; bsu:BSU25200; -.
DR   PATRIC; fig|224308.179.peg.2740; -.
DR   eggNOG; ENOG4105DG1; Bacteria.
DR   eggNOG; COG0568; LUCA.
DR   HOGENOM; HOG000270273; -.
DR   InParanoid; P06224; -.
DR   KO; K03086; -.
DR   OMA; YQRWLAE; -.
DR   PhylomeDB; P06224; -.
DR   BioCyc; BSUB:BSU25200-MONOMER; -.
DR   PRO; PR:P06224; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0001123; P:transcription initiation from bacterial-type RNA polymerase promoter; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.10; -; 2.
DR   Gene3D; 1.10.220.120; -; 1.
DR   HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR   InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR   InterPro; IPR028630; Sigma70_RpoD.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03979; Sigma70_r1_1; 1.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02393; RpoD_Cterm; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P06224.
DR   SWISS-2DPAGE; P06224.
KW   3D-structure; Cytoplasm; Direct protein sequencing; DNA-binding;
KW   Reference proteome; Sigma factor; Transcription; Transcription regulation.
FT   CHAIN           1..371
FT                   /note="RNA polymerase sigma factor SigA"
FT                   /id="PRO_0000093874"
FT   DNA_BIND        332..351
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          138..208
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          217..293
FT                   /note="Sigma-70 factor domain-3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   REGION          306..359
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00963"
FT   MOTIF           162..165
FT                   /note="Interaction with polymerase core subunit RpoC"
FT   CONFLICT        165
FT                   /note="Q -> H (in Ref. 1; AAA22709, 2; CAA27538 and 3;
FT                   BAA12489)"
FT                   /evidence="ECO:0000305"
FT   HELIX           13..27
FT                   /evidence="ECO:0000244|PDB:5MWW"
FT   STRAND          28..31
FT                   /evidence="ECO:0000244|PDB:5MWW"
FT   HELIX           32..38
FT                   /evidence="ECO:0000244|PDB:5MWW"
FT   HELIX           39..41
FT                   /evidence="ECO:0000244|PDB:5MWW"
FT   HELIX           46..59
FT                   /evidence="ECO:0000244|PDB:5MWW"
FT   STRAND          62..64
FT                   /evidence="ECO:0000244|PDB:5MWW"
SQ   SEQUENCE   371 AA;  42948 MW;  71EF04508FBF6471 CRC64;
     MADKQTHETE LTFDQVKEQL TESGKKRGVL TYEEIAERMS SFEIESDQMD EYYEFLGEQG
     VELISENEET EDPNIQQLAK AEEEFDLNDL SVPPGVKIND PVRMYLKEIG RVNLLSAKEE
     IAYAQKIEEG DEESKRRLAE ANLRLVVSIA KRYVGRGMLF LDLIQEGNMG LMKAVEKFDY
     RKGYKFSTYA TWWIRQAITR AIADQARTIR IPVHMVETIN KLIRVQRQLL QDLGREPTPE
     EIAEDMDLTP EKVREILKIA QEPVSLETPI GEEDDSHLGD FIEDQEATSP SDHAAYELLK
     EQLEDVLDTL TDREENVLRL RFGLDDGRTR TLEEVGKVFG VTRERIRQIE AKALRKLRHP
     SRSKRLKDFL E
//

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