(data stored in SCRATCH zone)

SWISSPROT: GLK_BACSU

ID   GLK_BACSU               Reviewed;         321 AA.
AC   P54495;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   11-DEC-2019, entry version 110.
DE   RecName: Full=Glucokinase;
DE            EC=2.7.1.2;
DE   AltName: Full=Glucose kinase;
GN   Name=glcK; Synonyms=yqgR; OrderedLocusNames=BSU24850;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 152 AND 198.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=9620975;
RA   Skarlatos P., Dahl M.K.;
RT   "The glucose kinase of Bacillus subtilis.";
RL   J. Bacteriol. 180:3222-3226(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.2;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the ROK (NagC/XylR) family. {ECO:0000305}.
DR   EMBL; D84432; BAA12521.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14416.2; -; Genomic_DNA.
DR   PIR; B69632; B69632.
DR   RefSeq; NP_390365.2; NC_000964.3.
DR   RefSeq; WP_003230132.1; NZ_JNCM01000036.1.
DR   SMR; P54495; -.
DR   STRING; 224308.BSU24850; -.
DR   jPOST; P54495; -.
DR   PaxDb; P54495; -.
DR   PRIDE; P54495; -.
DR   EnsemblBacteria; CAB14416; CAB14416; BSU24850.
DR   GeneID; 938206; -.
DR   KEGG; bsu:BSU24850; -.
DR   PATRIC; fig|224308.179.peg.2704; -.
DR   eggNOG; COG1940; LUCA.
DR   HOGENOM; HOG000150087; -.
DR   InParanoid; P54495; -.
DR   KO; K00845; -.
DR   OMA; GAPCNCG; -.
DR   PhylomeDB; P54495; -.
DR   BioCyc; BSUB:BSU24850-MONOMER; -.
DR   BioCyc; MetaCyc:MONOMER-5981; -.
DR   BRENDA; 2.7.1.2; 658.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004340; F:glucokinase activity; IMP:CACAO.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-KW.
DR   InterPro; IPR000600; ROK.
DR   InterPro; IPR004654; ROK_glcA.
DR   PANTHER; PTHR18964; PTHR18964; 1.
DR   Pfam; PF00480; ROK; 1.
DR   TIGRFAMs; TIGR00744; ROK_glcA_fam; 1.
DR   PROSITE; PS01125; ROK; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P54495.
DR   SWISS-2DPAGE; P54495.
KW   ATP-binding; Cytoplasm; Glycolysis; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..321
FT                   /note="Glucokinase"
FT                   /id="PRO_0000095676"
FT   CONFLICT        152
FT                   /note="V -> L (in Ref. 1; BAA12521)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        198
FT                   /note="E -> G (in Ref. 1; BAA12521)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   321 AA;  33604 MW;  F8162AE8BB49D3CD CRC64;
     MDEIWFAGID LGGTTIKLAF INQYGEIQHK WEVPTDKTGD TITVTIAKTI DSKLDELQKP
     KHIIKYIGMG APGPVDMAAG VVYETVNLGW KNYALKNHLE TETGIPAVIE NDANIAALGE
     MWKGAGDGAK DVILVTLGTG VGGGIIANGE IVHGINGAGG EIGHICSIPE GGAPCNCGKT
     GCIETIASAT GIVRIAKEKI ANAKKTTRLK ATEQLSARDV FEAAGENDEI ALEVVDYVAK
     HLGLVLGNLA SSLNPSKIVL GGGVSRAGEL LRSKVEKTFR KCAFPRAAQA ADISIAALGN
     DAGVIGGAWI AKNEWLKHQN C
//

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