(data stored in SCRATCH zone)

SWISSPROT: YQGS_BACSU

ID   YQGS_BACSU              Reviewed;         638 AA.
AC   P54496;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   11-DEC-2019, entry version 118.
DE   RecName: Full=Lipoteichoic acid synthase-like YqgS;
DE   Contains:
DE     RecName: Full=Uncharacterized protein YqgS;
DE   Contains:
DE     RecName: Full=Processed uncharacterized protein YqgS;
GN   Name=yqgS; OrderedLocusNames=BSU24840;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 7; 12; 301 AND 375.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NON-INVOLVEMENT IN LTA BIOSYNTHESIS.
RC   STRAIN=168;
RX   PubMed=17483484; DOI=10.1073/pnas.0701821104;
RA   Gruendling A., Schneewind O.;
RT   "Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus
RT   aureus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8478-8483(2007).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: [Processed uncharacterized protein YqgS]:
CC       Secreted {ECO:0000250}.
CC   -!- PTM: Proteolytically cleaved. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Although its sequence similarity with the glycerol
CC       phosphate lipoteichoic acid synthase LtaS, it cannot restore
CC       staphylococcal growth and LTA synthesis after ltaS depletion.
CC   -!- SIMILARITY: Belongs to the LTA synthase family. {ECO:0000305}.
DR   EMBL; D84432; BAA12522.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14415.2; -; Genomic_DNA.
DR   PIR; D69957; D69957.
DR   RefSeq; NP_390364.2; NC_000964.3.
DR   RefSeq; WP_004399146.1; NZ_JNCM01000036.1.
DR   SMR; P54496; -.
DR   STRING; 224308.BSU24840; -.
DR   PaxDb; P54496; -.
DR   PRIDE; P54496; -.
DR   EnsemblBacteria; CAB14415; CAB14415; BSU24840.
DR   GeneID; 938209; -.
DR   KEGG; bsu:BSU24840; -.
DR   PATRIC; fig|224308.179.peg.2703; -.
DR   eggNOG; ENOG4105E0M; Bacteria.
DR   eggNOG; COG1368; LUCA.
DR   HOGENOM; HOG000272195; -.
DR   InParanoid; P54496; -.
DR   OMA; FDPNNRY; -.
DR   PhylomeDB; P54496; -.
DR   BioCyc; BSUB:BSU24840-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008484; F:sulfuric ester hydrolase activity; IEA:InterPro.
DR   Gene3D; 3.40.720.10; -; 1.
DR   InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR   InterPro; IPR012160; LtaS-like.
DR   InterPro; IPR000917; Sulfatase_N.
DR   Pfam; PF00884; Sulfatase; 1.
DR   PIRSF; PIRSF005091; Mmb_sulf_HI1246; 1.
DR   SUPFAM; SSF53649; SSF53649; 1.
PE   3: Inferred from homology;
DR   PRODOM; P54496.
DR   SWISS-2DPAGE; P54496.
KW   Cell membrane; Manganese; Membrane; Metal-binding; Reference proteome;
KW   Secreted; Transmembrane; Transmembrane helix.
FT   CHAIN           1..215
FT                   /note="Uncharacterized protein YqgS"
FT                   /id="PRO_0000049814"
FT   CHAIN           216..638
FT                   /note="Processed uncharacterized protein YqgS"
FT                   /id="PRO_0000305376"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..41
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        63..70
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        92..116
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        138..153
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        175..638
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        295
FT                   /evidence="ECO:0000250"
FT   METAL           253
FT                   /note="Manganese"
FT                   /evidence="ECO:0000250"
FT   METAL           295
FT                   /note="Manganese"
FT                   /evidence="ECO:0000250"
FT   METAL           469
FT                   /note="Manganese"
FT                   /evidence="ECO:0000250"
FT   METAL           470
FT                   /note="Manganese"
FT                   /evidence="ECO:0000250"
FT   BINDING         410
FT                   /note="Substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            215..216
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        7
FT                   /note="S -> P (in Ref. 1; BAA12522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        12
FT                   /note="M -> T (in Ref. 1; BAA12522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        301
FT                   /note="I -> N (in Ref. 1; BAA12522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        375
FT                   /note="S -> T (in Ref. 1; BAA12522)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   638 AA;  73212 MW;  6CEDC0D558E2ED31 CRC64;
     MRKTFFSKIS FMLIAILLMW LKTYAVYKTS FHIKIDNLTQ EFILFINPLS FLLLIFGLSL
     FLKGKNRNRY IIAMSCLVTF VLLANMVFYR FYNDFLTIPV LFQTSNMGDL GSSIGTLLEP
     TDLLLAVDIA VLIWLHIRQK AFQSDIPSTK NERAAYFLFV ASVYFFNLGL SEAERPQLLT
     RSFDREMLVK NISLFNFHIY DGVLQSKQSA QRALADSNSL TEIENYVTAN AKDANKRLFG
     AAKGRNVILV SLESTQSFVI NEKLNGEEIT PFLNDFIKQS YNFNNVYHQT GQGKTSDSEF
     IVDNSLYPLG RGAVFFTNAG NQYMAAPEIL KNSGYYSAVL HANNKSFWNR DLMYDSFGYD
     SFFDINSYDV TDENSVGWGL KDKEFFEQSS ELMKNLPQPF YSRLITLTNH FPFDLDEEDQ
     LIDEYDSNSQ TLNKYFPTVR YQDEALKRFI EKLKEDGLYD NSVIVLYGDH YGISENHNEA
     MGQFLGKEIT PFEEVQLQKV PLVIHIPGIT DKKPQTIETV GGQIDIRPTL MNLLGIDTKD
     QIQFGNDLLS DEKLDFTVLR DGSFITDQVV YTDGACYDKE TGKPLEETKQ CEAFADKAKQ
     ELSLSDEIIY GDLLRFYDQK RLDNSSKRKE KQMLDQAS
//

If you have problems or comments...

PBIL Back to PBIL home page