(data stored in SCRATCH zone)

SWISSPROT: SP3AF_BACSU

ID   SP3AF_BACSU             Reviewed;         206 AA.
AC   P49783;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   11-DEC-2019, entry version 101.
DE   RecName: Full=Stage III sporulation protein AF;
GN   Name=spoIIIAF; OrderedLocusNames=BSU24380;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RA   Guerout-Fleury A.M., Gonzy-Treboul G., Stragier P.;
RL   Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
DR   EMBL; U35252; AAA76725.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12565.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14369.1; -; Genomic_DNA.
DR   PIR; A69712; A69712.
DR   RefSeq; NP_390318.1; NC_000964.3.
DR   RefSeq; WP_003230233.1; NZ_JNCM01000036.1.
DR   PDB; 6DCS; X-ray; 2.70 A; A/B=85-206.
DR   PDBsum; 6DCS; -.
DR   SMR; P49783; -.
DR   STRING; 224308.BSU24380; -.
DR   TCDB; 9.B.70.1.1; the multicomponent putative spoiiiae exporter (spoiiia-e) family.
DR   PaxDb; P49783; -.
DR   PRIDE; P49783; -.
DR   EnsemblBacteria; CAB14369; CAB14369; BSU24380.
DR   GeneID; 938576; -.
DR   KEGG; bsu:BSU24380; -.
DR   PATRIC; fig|224308.179.peg.2656; -.
DR   eggNOG; ENOG410644B; Bacteria.
DR   eggNOG; ENOG41126UT; LUCA.
DR   HOGENOM; HOG000096694; -.
DR   KO; K06395; -.
DR   OMA; IWEIGSE; -.
DR   BioCyc; BSUB:BSU24380-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR014245; Spore_III_AF.
DR   Pfam; PF09581; Spore_III_AF; 1.
DR   TIGRFAMs; TIGR02896; spore_III_AF; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P49783.
DR   SWISS-2DPAGE; P49783.
KW   3D-structure; Cell membrane; Membrane; Reference proteome; Sporulation;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..206
FT                   /note="Stage III sporulation protein AF"
FT                   /id="PRO_0000072070"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   STRAND          85..88
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   HELIX           91..110
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   STRAND          112..115
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   STRAND          117..125
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   HELIX           132..134
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   STRAND          135..143
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   HELIX           161..186
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   HELIX           190..192
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   STRAND          193..196
FT                   /evidence="ECO:0000244|PDB:6DCS"
FT   STRAND          198..201
FT                   /evidence="ECO:0000244|PDB:6DCS"
SQ   SEQUENCE   206 AA;  23291 MW;  BD47F355B9F3D35D CRC64;
     MSFLTEWLTT IVLFILFAIV IDMLLPSSSM QKYAKMVVSL LLIVVMLTPI FKLFKTDPEV
     IFEYLTKNGQ SESADIKNQI NSKKIEIQAS QRAYILEEMA VQLKKKAEER FSHDEYKVGR
     IKLTAGEKVD SEEDIKTISV YMAPSSEKTV QTVAPVHIDT DHAYVTKEAA EQKEAKQIQT
     QLADIWEIGS EKITVHMEGG ESVGNE
//

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