(data stored in SCRATCH zone)

SWISSPROT: BMR1_BACSU

ID   BMR1_BACSU              Reviewed;         389 AA.
AC   P33449;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   11-DEC-2019, entry version 126.
DE   RecName: Full=Multidrug resistance protein 1;
DE   AltName: Full=Multidrug-efflux transporter 1;
GN   Name=bmr; Synonyms=bmr1; OrderedLocusNames=BSU24010;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / BD170;
RX   PubMed=1675788; DOI=10.1073/pnas.88.11.4781;
RA   Neyfakh A.A., Bidnenko V.E., Chen L.B.;
RT   "Efflux-mediated multidrug resistance in Bacillus subtilis: similarities
RT   and dissimilarities with the mammalian system.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:4781-4785(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7961792;
RA   Ahmed M., Borsch C.M., Taylor S.S., Vazquez-Laslop N., Neyfakh A.A.;
RT   "A protein that activates expression of a multidrug efflux transporter upon
RT   binding the transporter substrates.";
RL   J. Biol. Chem. 269:28506-28513(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   SEQUENCE REVISION TO 109.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- FUNCTION: Energy-dependent efflux pump responsible for decreased drug
CC       accumulation in multi-drug-resistant cells. Probably uses a
CC       transmembrane proton gradient as the energy source. Causes the efflux
CC       of a variety of toxic substances, including such structurally diverse
CC       compounds as ethidium bromide, rhodamine and acridine dyes,
CC       tetraphenylphosphonium, puromycin, chloramphenicol, doxorubicin, and
CC       fluoroquinolone antibiotics.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
DR   EMBL; M33768; AAA22277.1; -; Genomic_DNA.
DR   EMBL; L25604; AAB81539.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12601.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14332.2; -; Genomic_DNA.
DR   PIR; A39705; A39705.
DR   RefSeq; NP_390281.2; NC_000964.3.
DR   RefSeq; WP_003230328.1; NZ_JNCM01000036.1.
DR   STRING; 224308.BSU24010; -.
DR   TCDB; 2.A.1.2.70; the major facilitator superfamily (mfs).
DR   PaxDb; P33449; -.
DR   PRIDE; P33449; -.
DR   EnsemblBacteria; CAB14332; CAB14332; BSU24010.
DR   GeneID; 938680; -.
DR   KEGG; bsu:BSU24010; -.
DR   PATRIC; fig|224308.179.peg.2615; -.
DR   eggNOG; COG0477; LUCA.
DR   HOGENOM; HOG000266878; -.
DR   InParanoid; P33449; -.
DR   KO; K19578; -.
DR   OMA; DTLWFGY; -.
DR   PhylomeDB; P33449; -.
DR   BioCyc; BSUB:BSU24010-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR   Pfam; PF07690; MFS_1; 1.
DR   PRINTS; PR01035; TCRTETA.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE   3: Inferred from homology;
DR   PRODOM; P33449.
DR   SWISS-2DPAGE; P33449.
KW   Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..389
FT                   /note="Multidrug resistance protein 1"
FT                   /id="PRO_0000173317"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        336..356
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        109
FT                   /note="A -> P (in Ref. 1; AAA22277, 2; AAB81539 and 3;
FT                   BAA12601)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   389 AA;  42232 MW;  3E0318AB533D1197 CRC64;
     MEKKNITLTI LLTNLFIAFL GIGLVIPVTP TIMNELHLSG TAVGYMVACF AITQLIVSPI
     AGRWVDRFGR KIMIVIGLLF FSVSEFLFGI GKTVEMLFIS RMLGGISAAF IMPGVTAFIA
     DITTIKTRPK ALGYMSAAIS TGFIIGPGIG GFLAEVHSRL PFFFAAAFAL LAAILSILTL
     REPERNPENQ EIKGQKTGFK RIFAPMYFIA FLIILISSFG LASFESLFAL FVDHKFGFTA
     SDIAIMITGG AIVGAITQVV LFDRFTRWFG EIHLIRYSLI LSTSLVFLLT TVHSYVAILL
     VTVTVFVGFD LMRPAVTTYL SKIAGNEQGF AGGMNSMFTS IGNVFGPIIG GMLFDIDVNY
     PFYFATVTLA IGIALTIAWK APAHLKAST
//

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