(data stored in SCRATCH zone)

SWISSPROT: XERD_BACSU

ID   XERD_BACSU              Reviewed;         296 AA.
AC   P46352;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 3.
DT   11-DEC-2019, entry version 132.
DE   RecName: Full=Tyrosine recombinase XerD;
GN   Name=xerD; Synonyms=ripX, ykqM; OrderedLocusNames=BSU23510;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 255.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 133-296.
RC   STRAIN=168 / BR151;
RX   PubMed=10537218; DOI=10.1099/00221287-145-10-2957;
RA   Schuch R., Garibian A., Saxild H.H., Piggot P.J., Nygaard P.;
RT   "Nucleosides as a carbon source in Bacillus subtilis: characterization of
RT   the drm-pupG operon.";
RL   Microbiology 145:2957-2966(1999).
RN   [5]
RP   FUNCTION, AND ENZYME ACTIVITY.
RX   PubMed=10498718;
RA   Sciochetti S.A., Piggot P.J., Sherratt D.J., Blakely G.;
RT   "The ripX locus of Bacillus subtilis encodes a site-specific recombinase
RT   involved in proper chromosome partitioning.";
RL   J. Bacteriol. 181:6053-6062(1999).
CC   -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC       the cutting and rejoining of the recombining DNA molecules. The XerC-
CC       XerD complex is essential to convert dimers of the bacterial chromosome
CC       into monomers to permit their segregation at cell division. It also
CC       contributes to the segregational stability of plasmids.
CC       {ECO:0000269|PubMed:10498718}.
CC   -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC       molecules of XerC and two molecules of XerD. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. XerD subfamily.
CC       {ECO:0000305}.
DR   EMBL; D84432; BAA12649.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14283.2; -; Genomic_DNA.
DR   EMBL; U32685; AAA74432.1; -; Genomic_DNA.
DR   PIR; A69693; A69693.
DR   RefSeq; NP_390232.2; NC_000964.3.
DR   RefSeq; WP_004398985.1; NZ_JNCM01000036.1.
DR   SMR; P46352; -.
DR   STRING; 224308.BSU23510; -.
DR   PaxDb; P46352; -.
DR   PRIDE; P46352; -.
DR   EnsemblBacteria; CAB14283; CAB14283; BSU23510.
DR   GeneID; 938726; -.
DR   KEGG; bsu:BSU23510; -.
DR   PATRIC; fig|224308.179.peg.2561; -.
DR   eggNOG; ENOG4105C13; Bacteria.
DR   eggNOG; COG4974; LUCA.
DR   HOGENOM; HOG000045296; -.
DR   InParanoid; P46352; -.
DR   KO; K04763; -.
DR   OMA; FASHMLE; -.
DR   PhylomeDB; P46352; -.
DR   BioCyc; BSUB:BSU23510-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR   HAMAP; MF_01807; Recomb_XerD; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   InterPro; IPR011932; Recomb_XerD.
DR   InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR   Pfam; PF02899; Phage_int_SAM_1; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   TIGRFAMs; TIGR02225; recomb_XerD; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
DR   PRODOM; P46352.
DR   SWISS-2DPAGE; P46352.
KW   Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW   DNA integration; DNA recombination; DNA-binding; Reference proteome.
FT   CHAIN           1..296
FT                   /note="Tyrosine recombinase XerD"
FT                   /id="PRO_0000095371"
FT   DOMAIN          1..86
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          107..290
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        147
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        171
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        242
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        245
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        268
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        277
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        215..235
FT                   /note="KQISRQGFWKNLKKIALEAGI -> NRSAARILEEPEKNRIGSRH (in
FT                   Ref. 4; AAA74432)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        255
FT                   /note="N -> D (in Ref. 1; BAA12649)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   296 AA;  33994 MW;  0541E31F3ECC378E CRC64;
     MKDQIKDFIH YVMVERGLSQ NTIVSYERDL KSYSLYLTET LHVTDWNHVT RIHIIQYLKH
     LKDSGKSGKT SARHLASIRS FHQFLLREKV TDKDPSVHIE TQKTERALPK VLALNEVERL
     LDTPKLTSPF GYRDKAMLEL LYATGIRVSE MIELKTADVH LSMGFIRCFG KGRKERIVPI
     GEAAASAIEE YMTKARGKLL KNNVSDALFL NHHGKQISRQ GFWKNLKKIA LEAGIKKELT
     PHTLRHSFAT HLLENGADLR AVQEMLGHAD ISTTQIYTHV TKTRLKDVYK QFHPRA
//

If you have problems or comments...

PBIL Back to PBIL home page