(data stored in SCRATCH zone)

SWISSPROT: SP5AF_BACSU

ID   SP5AF_BACSU             Reviewed;         493 AA.
AC   P31845;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 4.
DT   11-DEC-2019, entry version 113.
DE   RecName: Full=Stage V sporulation protein AF;
GN   Name=spoVAF; OrderedLocusNames=BSU23390;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 410-438.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-493.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / VKM B-501;
RX   PubMed=7934829; DOI=10.1111/j.1365-2958.1993.tb02670.x;
RA   Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P.;
RT   "The organization of the Bacillus subtilis 168 chromosome region between
RT   the spoVA and serA genetic loci, based on sequence data.";
RL   Mol. Microbiol. 10:385-395(1993).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 223-493.
RX   PubMed=1368705; DOI=10.1271/bbb1961.55.1615;
RA   Yamamoto J., Shimizu M., Yamane K.;
RT   "Molecular cloning and analysis of nucleotide sequence of the Bacillus
RT   subtilis lysA gene region using B. subtilis phage vectors and a multi-copy
RT   plasmid, pUB110.";
RL   Agric. Biol. Chem. 55:1615-1626(1991).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82.
RX   PubMed=3926949; DOI=10.1099/00221287-131-5-1091;
RA   Fort P., Errington J.;
RT   "Nucleotide sequence and complementation analysis of a polycistronic
RT   sporulation operon, spoVA, in Bacillus subtilis.";
RL   J. Gen. Microbiol. 131:1091-1105(1985).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GerABKA family. {ECO:0000305}.
DR   EMBL; D84432; BAA12661.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14271.2; -; Genomic_DNA.
DR   EMBL; L09228; AAA67472.1; -; Genomic_DNA.
DR   EMBL; D90189; BAA14210.1; -; Genomic_DNA.
DR   EMBL; M15349; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A69715; A69715.
DR   RefSeq; NP_390220.2; NC_000964.3.
DR   RefSeq; WP_003230471.1; NZ_JNCM01000036.1.
DR   RefSeq; WP_009967659.1; NZ_CM000487.1.
DR   STRING; 224308.BSU23390; -.
DR   TCDB; 9.A.11.1.1; the dipicolinic acid transporter (dpa-t) family.
DR   PRIDE; P31845; -.
DR   EnsemblBacteria; CAB14271; CAB14271; BSU23390.
DR   GeneID; 938936; -.
DR   KEGG; bsu:BSU23390; -.
DR   PATRIC; fig|224308.179.peg.2548; -.
DR   HOGENOM; HOG000059246; -.
DR   KO; K06408; -.
DR   OMA; NTPYLWP; -.
DR   PhylomeDB; P31845; -.
DR   BioCyc; BSUB:BSU23390-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009847; P:spore germination; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR004995; Spore_Ger.
DR   Pfam; PF03323; GerA; 1.
DR   PIRSF; PIRSF005690; GerBA; 1.
PE   3: Inferred from homology;
DR   PRODOM; P31845.
DR   SWISS-2DPAGE; P31845.
KW   Cell membrane; Membrane; Reference proteome; Sporulation; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..493
FT                   /note="Stage V sporulation protein AF"
FT                   /id="PRO_0000164019"
FT   TRANSMEM        296..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        363..383
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        387..407
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        410..438
FT                   /note="NKMSRLVLMILVALFHIKGLVIGFTVLII -> TNEPSCPHDTRCFISYKRA
FT                   RHRIYSANY (in Ref. 1; BAA12661 and 4; AAA67472)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        410..438
FT                   /note="NKMSRLVLMILVALFHIKGLVIGFTVLII -> TNEPSCPHDTRCFISYKRA
FT                   RHRLYSANY (in Ref. 5; BAA14210)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        468
FT                   /note="R -> P (in Ref. 5; BAA14210)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   493 AA;  55473 MW;  5357B730F91361CE CRC64;
     MPDHKEEKIR VYRNPAKNEE YFKNRVGMGT SYDVGVRKLT ILDKEIQLYY LNGLCDTAYI
     IHLMRELVAI NNRKEDPDEL VDIVENRLLN AQVEKVKTLD ETTDQVLSGL VAVIVEGAGF
     AFIIDVRSYP GRNPEEPDTE KVVRGARDGF VENIVVNTAL LRRRIRDERL RVKMTKVGER
     SKTDLSICYI EDIADPDLVE IVEKEIASID VDGLTMADKT VEEFIVNQSY NPFPLVRYTE
     RPDVAANHVL EGHVIIIVDT SPSVIITPTT LFHHVQHAEE YRQAPSVGTF LRWVRFFGIL
     ASTLFLPIWF LFVLQPDLLP DNMKFIGLNK DTHIPIILQI FLADLGIEFL RMAAIHTPTA
     LSTAMGLIAA VLIGQIAIEV GLFSPEVILY VSLAAIGTFT TPSYELSLAN KMSRLVLMIL
     VALFHIKGLV IGFTVLIIAM ASIKSLQTPY LWPLIPFNGK ALWQVLVRTA KPGAKVRPSI
     VHPKNRLRQP TNS
//

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