(data stored in ACNUC7421 zone)

SWISSPROT: ENDAI_BACSU

ID   ENDAI_BACSU             Reviewed;          93 AA.
AC   P96621; Q797K5;
DT   02-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   11-DEC-2019, entry version 102.
DE   RecName: Full=Antitoxin EndoAI {ECO:0000303|PubMed:15882409};
DE   AltName: Full=Antitoxin MazE-bs;
DE            Short=MazE-bs;
DE   AltName: Full=EndoA inhibitor;
GN   Name=ndoAI {ECO:0000303|PubMed:15882409}; Synonyms=mazE;
GN   OrderedLocusNames=BSU04650;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION AS AN ANTITOXIN, SUBUNIT, AND EXPRESSION IN E.COLI.
RC   STRAIN=168;
RX   PubMed=15882409; DOI=10.1111/j.1365-2958.2005.04606.x;
RA   Pellegrini O., Mathy N., Gogos A., Shapiro L., Condon C.;
RT   "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the
RT   MazF/PemK family and its inhibitor.";
RL   Mol. Microbiol. 56:1139-1148(2005).
RN   [4]
RP   FUNCTION AS AN ANTITOXIN, AND EXPRESSION IN E.COLI.
RC   STRAIN=168;
RX   PubMed=21763692; DOI=10.1016/j.febslet.2011.07.008;
RA   Park J.H., Yamaguchi Y., Inouye M.;
RT   "Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.";
RL   FEBS Lett. 585:2526-2532(2011).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 2-93 IN COMPLEX WITH MAZF,
RP   FUNCTION, SUBUNIT, AND MUTAGENESIS OF TYR-61; MET-64; ASN-68; SER-72;
RP   GLU-74 AND GLU-79.
RC   STRAIN=168;
RX   PubMed=24120662; DOI=10.1016/j.molcel.2013.09.006;
RA   Simanshu D.K., Yamaguchi Y., Park J.H., Inouye M., Patel D.J.;
RT   "Structural basis of mRNA recognition and cleavage by toxin MazF and its
RT   regulation by antitoxin MazE in Bacillus subtilis.";
RL   Mol. Cell 52:447-458(2013).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Antitoxin that directly inhibits activity of EndoA in vitro. Upon
CC       expression in E.coli counteracts inhibitory effect of endoribonuclease
CC       EndoA. The EndoA-EndoAI complex does not seem to bind its own promoter
CC       (PubMed:24120662). {ECO:0000269|PubMed:15882409,
CC       ECO:0000269|PubMed:21763692, ECO:0000269|PubMed:24120662}.
CC   -!- SUBUNIT: Homodimer, forms a heterohexamer composed of alternating toxin
CC       and antitoxin homodimers which inhibits the toxin's endoribonuclease
CC       activity. Antitoxin prevents RNA binding to the endoribonuclease
CC       (PubMed:24120662). {ECO:0000269|PubMed:15882409,
CC       ECO:0000269|PubMed:24120662}.
CC   -!- INTERACTION:
CC       Self; NbExp=2; IntAct=EBI-7370294, EBI-7370294;
CC   -!- SIMILARITY: Belongs to the MazE/EndoAI family. {ECO:0000305}.
DR   EMBL; AB001488; BAA19302.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12272.1; -; Genomic_DNA.
DR   PIR; B69773; B69773.
DR   RefSeq; NP_388346.1; NC_000964.3.
DR   RefSeq; WP_003225183.1; NZ_JNCM01000031.1.
DR   PDB; 4ME7; X-ray; 2.92 A; E/F=2-93.
DR   PDBsum; 4ME7; -.
DR   SMR; P96621; -.
DR   MINT; P96621; -.
DR   STRING; 224308.BSU04650; -.
DR   PaxDb; P96621; -.
DR   PRIDE; P96621; -.
DR   EnsemblBacteria; CAB12272; CAB12272; BSU04650.
DR   GeneID; 938177; -.
DR   KEGG; bsu:BSU04650; -.
DR   PATRIC; fig|224308.179.peg.493; -.
DR   eggNOG; COG0864; LUCA.
DR   HOGENOM; HOG000269595; -.
DR   KO; K07723; -.
DR   OMA; AMKLYIA; -.
DR   PhylomeDB; P96621; -.
DR   BioCyc; BSUB:BSU04650-MONOMER; -.
DR   PRO; PR:P96621; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR002145; CopG.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   Pfam; PF01402; RHH_1; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P96621.
DR   SWISS-2DPAGE; P96621.
KW   3D-structure; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..93
FT                   /note="Antitoxin EndoAI"
FT                   /id="PRO_0000086974"
FT   MUTAGEN         61
FT                   /note="Y->A: No longer prevents EndoA toxicity upon
FT                   coexpression in E.coli, due to loss of EndoA-EndoAI
FT                   interaction."
FT                   /evidence="ECO:0000269|PubMed:24120662"
FT   MUTAGEN         64
FT                   /note="M->A: Still prevents EndoA toxicity upon
FT                   coexpression in E.coli."
FT                   /evidence="ECO:0000269|PubMed:24120662"
FT   MUTAGEN         68
FT                   /note="N->A: Still prevents EndoA toxicity upon
FT                   coexpression in E.coli."
FT                   /evidence="ECO:0000269|PubMed:24120662"
FT   MUTAGEN         72
FT                   /note="S->A: Still prevents EndoA toxicity upon
FT                   coexpression in E.coli."
FT                   /evidence="ECO:0000269|PubMed:24120662"
FT   MUTAGEN         74
FT                   /note="E->A: Still prevents EndoA toxicity upon
FT                   coexpression in E.coli."
FT                   /evidence="ECO:0000269|PubMed:24120662"
FT   MUTAGEN         79
FT                   /note="E->A: Still prevents EndoA toxicity upon
FT                   coexpression in E.coli."
FT                   /evidence="ECO:0000269|PubMed:24120662"
FT   STRAND          10..13
FT                   /evidence="ECO:0000244|PDB:4ME7"
FT   HELIX           16..29
FT                   /evidence="ECO:0000244|PDB:4ME7"
FT   HELIX           33..74
FT                   /evidence="ECO:0000244|PDB:4ME7"
FT   HELIX           76..81
FT                   /evidence="ECO:0000244|PDB:4ME7"
SQ   SEQUENCE   93 AA;  10547 MW;  FA77E052B42BC28D CRC64;
     MSESSARTEM KISLPENLVA ELDGVAMREK RSRNELISQA VRAYVSERTT RHNRDLMRRG
     YMEMAKINLN ISSEAHFAEC EAETTVERLV SGG
//

If you have problems or comments...

PBIL Back to PBIL home page