(data stored in ACNUC7421 zone)

SWISSPROT: MNEP_BACSU

ID   MNEP_BACSU              Reviewed;         297 AA.
AC   C0SP78; P96691; Q797G0;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   11-DEC-2019, entry version 60.
DE   RecName: Full=Manganese efflux system protein MneP {ECO:0000303|PubMed:27748968};
GN   Name=mneP {ECO:0000303|PubMed:27748968}; Synonyms=ydfM;
GN   OrderedLocusNames=BSU05470;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT   "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT   subtilis genome.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 209.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=27748968; DOI=10.1111/mmi.13554;
RA   Huang X., Shin J.H., Pinochet-Barros A., Su T.T., Helmann J.D.;
RT   "Bacillus subtilis MntR coordinates the transcriptional regulation of
RT   manganese uptake and efflux systems.";
RL   Mol. Microbiol. 103:253-268(2017).
CC   -!- FUNCTION: Primary efflux pump for manganese. May prevent manganese
CC       intoxication. {ECO:0000269|PubMed:27748968}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Transcriptionally activated by MntR in response to manganese
CC       excess. Expressed at a basal level in the absence of MntR.
CC       {ECO:0000269|PubMed:27748968}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant is manganese sensitive and
CC       accumulates high levels of intracellular manganese. Effects are
CC       exacerbated in a mneP-mneS double mutant.
CC       {ECO:0000269|PubMed:27748968}.
CC   -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC       transporter (TC 2.A.4) family. {ECO:0000305}.
DR   EMBL; AB001488; BAA19381.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12354.2; -; Genomic_DNA.
DR   PIR; C69781; C69781.
DR   RefSeq; NP_388428.2; NC_000964.3.
DR   RefSeq; WP_003244477.1; NZ_JNCM01000031.1.
DR   STRING; 224308.BSU05470; -.
DR   PaxDb; C0SP78; -.
DR   PRIDE; C0SP78; -.
DR   EnsemblBacteria; CAB12354; CAB12354; BSU05470.
DR   GeneID; 939900; -.
DR   KEGG; bsu:BSU05470; -.
DR   PATRIC; fig|224308.179.peg.587; -.
DR   eggNOG; ENOG41076HS; Bacteria.
DR   eggNOG; COG0053; LUCA.
DR   HOGENOM; HOG000062182; -.
DR   InParanoid; C0SP78; -.
DR   OMA; HPYGHQR; -.
DR   PhylomeDB; C0SP78; -.
DR   BioCyc; BSUB:BSU05470-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005623; C:cell; IEA:GOC.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015093; F:ferrous iron transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015341; F:zinc efflux active transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006876; P:cellular cadmium ion homeostasis; IBA:GO_Central.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IBA:GO_Central.
DR   GO; GO:0006882; P:cellular zinc ion homeostasis; IBA:GO_Central.
DR   Gene3D; 1.20.1510.10; -; 1.
DR   Gene3D; 3.30.70.1350; -; 1.
DR   InterPro; IPR002524; Cation_efflux.
DR   InterPro; IPR027470; Cation_efflux_CTD.
DR   InterPro; IPR036837; Cation_efflux_CTD_sf.
DR   InterPro; IPR027469; Cation_efflux_TMD_sf.
DR   Pfam; PF01545; Cation_efflux; 1.
DR   Pfam; PF16916; ZT_dimer; 1.
DR   SUPFAM; SSF160240; SSF160240; 1.
DR   SUPFAM; SSF161111; SSF161111; 1.
DR   TIGRFAMs; TIGR01297; CDF; 1.
PE   2: Evidence at transcript level;
DR   PRODOM; C0SP78.
DR   SWISS-2DPAGE; C0SP78.
KW   Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..297
FT                   /note="Manganese efflux system protein MneP"
FT                   /id="PRO_0000378462"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        209
FT                   /note="D -> G (in Ref. 1; BAA19381)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   297 AA;  32854 MW;  901A9FDA4B889E40 CRC64;
     MASEREQISR KVALIALIAN LILMAGKVFF GLVGDSEAVF ADGIHSAADV VASIAVLAVI
     GISNKPPDQD HPFGHGKAEV ISEAIVGIIL VIVSVYILIE AILSFVKGPS VPQYSALFAA
     LISYVAKEIL YRYSIKQGKK WNSKAIIAIA YDHKGDIVAS LAAFIGVLLA IIGNSRGWSY
     LLYADAIASA IVAYLIFKIS MELIRPSVDV LMEKSVDPEL IEEYKAVIFQ CDQVKRIDRI
     RAREHGHYKL LDVRLSLDHD LTIKQGHDIA REIRNEIKRQ FSDVEEVLIH VNPYFEE
//

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