(data stored in ACNUC7421 zone)

SWISSPROT: RIBF_ECO57

ID   RIBF_ECO57              Reviewed;         313 AA.
AC   P0AG42; P08391; P75621;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   05-JUL-2017, entry version 79.
DE   RecName: Full=Riboflavin biosynthesis protein RibF;
DE   Includes:
DE     RecName: Full=Riboflavin kinase;
DE              EC=2.7.1.26;
DE     AltName: Full=Flavokinase;
DE   Includes:
DE     RecName: Full=FMN adenylyltransferase;
DE              EC=2.7.7.2;
DE     AltName: Full=FAD pyrophosphorylase;
DE     AltName: Full=FAD synthase;
GN   Name=ribF; OrderedLocusNames=Z0029, ECs0028;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- CATALYTIC ACTIVITY: ATP + riboflavin = ADP + FMN.
CC   -!- CATALYTIC ACTIVITY: ATP + FMN = diphosphate + FAD.
CC   -!- PATHWAY: Cofactor biosynthesis; FAD biosynthesis; FAD from FMN:
CC       step 1/1.
CC   -!- PATHWAY: Cofactor biosynthesis; FMN biosynthesis; FMN from
CC       riboflavin (ATP route): step 1/1.
CC   -!- SIMILARITY: Belongs to the RibF family. {ECO:0000305}.
DR   EMBL; AE005174; AAG54327.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33451.1; -; Genomic_DNA.
DR   PIR; C85483; C85483.
DR   PIR; D90632; D90632.
DR   RefSeq; NP_308055.1; NC_002695.1.
DR   RefSeq; WP_000767329.1; NZ_MWVM01000003.1.
DR   ProteinModelPortal; P0AG42; -.
DR   SMR; P0AG42; -.
DR   MINT; MINT-1256494; -.
DR   STRING; 155864.Z0029; -.
DR   PRIDE; P0AG42; -.
DR   EnsemblBacteria; AAG54327; AAG54327; Z0029.
DR   EnsemblBacteria; BAB33451; BAB33451; BAB33451.
DR   GeneID; 913423; -.
DR   KEGG; ece:Z0029; -.
DR   KEGG; ecs:ECs0028; -.
DR   PATRIC; fig|386585.9.peg.123; -.
DR   eggNOG; ENOG4105DTN; Bacteria.
DR   eggNOG; COG0196; LUCA.
DR   HOGENOM; HOG000006845; -.
DR   KO; K11753; -.
DR   UniPathway; UPA00276; UER00406.
DR   UniPathway; UPA00277; UER00407.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003919; F:FMN adenylyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008531; F:riboflavin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006747; P:FAD biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009398; P:FMN biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009231; P:riboflavin biosynthetic process; IEA:InterPro.
DR   Gene3D; 2.40.30.30; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR015864; FAD_synthase.
DR   InterPro; IPR023468; Riboflavin_kinase.
DR   InterPro; IPR002606; Riboflavin_kinase_bac.
DR   InterPro; IPR015865; Riboflavin_kinase_bac/euk.
DR   InterPro; IPR023465; Riboflavin_kinase_domain.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR22749; PTHR22749; 1.
DR   Pfam; PF06574; FAD_syn; 1.
DR   Pfam; PF01687; Flavokinase; 1.
DR   PIRSF; PIRSF004491; FAD_Synth; 1.
DR   SMART; SM00904; Flavokinase; 1.
DR   SUPFAM; SSF82114; SSF82114; 1.
DR   TIGRFAMs; TIGR00083; ribF; 1.
PE   3: Inferred from homology;
DR   PRODOM; P0AG42.
DR   SWISS-2DPAGE; P0AG42.
KW   ATP-binding; Complete proteome; FAD; Flavoprotein; FMN; Kinase;
KW   Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase;
KW   Transferase.
FT   CHAIN         1    313       Riboflavin biosynthesis protein RibF.
FT                                /FTId=PRO_0000194138.
SQ   SEQUENCE   313 AA;  34734 MW;  C0B2EF5499CD30FE CRC64;
     MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV MVMLFEPQPL
     ELFATDKAPA RLTRLREKLR YLAECGVDYV LCVRFDRRFA ALTAQNFISD LLVKHLRVKF
     LAVGDDFRFG AGREGDFLLL QKAGMEYGFD ITSTQTFCEG GVRISSTAVR QALADDNLAL
     AESLLGHPFA ISGRVVHGDE LGRTIGFPTA NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV
     ANIGTRPTVA GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI RNEQRFASLD ELKAQIARDE
     LTAREFFGLT KPA
//

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