(data stored in ACNUC7421 zone)

SWISSPROT: KEFF_ECO57

ID   KEFF_ECO57              Reviewed;         176 AA.
AC   Q8XA24;
DT   03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   05-JUL-2017, entry version 86.
DE   RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefF {ECO:0000255|HAMAP-Rule:MF_01414};
DE   AltName: Full=Quinone oxidoreductase KefF {ECO:0000255|HAMAP-Rule:MF_01414};
DE            EC=1.6.5.2 {ECO:0000255|HAMAP-Rule:MF_01414};
GN   Name=kefF {ECO:0000255|HAMAP-Rule:MF_01414};
GN   OrderedLocusNames=Z0052, ECs0049;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Regulatory subunit of a potassium efflux system that
CC       confers protection against electrophiles. Required for full
CC       activity of KefC. Shows redox enzymatic activity, but this
CC       enzymatic activity is not required for activation of KefC.
CC       {ECO:0000255|HAMAP-Rule:MF_01414}.
CC   -!- CATALYTIC ACTIVITY: NAD(P)H + a quinone = NAD(P)(+) + a
CC       hydroquinone. {ECO:0000255|HAMAP-Rule:MF_01414}.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01414};
CC   -!- SUBUNIT: Homodimer. Interacts with KefC. {ECO:0000255|HAMAP-
CC       Rule:MF_01414}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01414}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01414}; Cytoplasmic side {ECO:0000255|HAMAP-
CC       Rule:MF_01414}.
CC   -!- SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family.
CC       KefF subfamily. {ECO:0000255|HAMAP-Rule:MF_01414}.
DR   EMBL; AE005174; AAG54349.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33472.1; -; Genomic_DNA.
DR   PIR; A85486; A85486.
DR   PIR; A90635; A90635.
DR   RefSeq; NP_308076.1; NC_002695.1.
DR   RefSeq; WP_000600700.1; NZ_MWVM01000003.1.
DR   ProteinModelPortal; Q8XA24; -.
DR   SMR; Q8XA24; -.
DR   STRING; 155864.Z0052; -.
DR   EnsemblBacteria; AAG54349; AAG54349; Z0052.
DR   EnsemblBacteria; BAB33472; BAB33472; BAB33472.
DR   GeneID; 913448; -.
DR   KEGG; ece:Z0052; -.
DR   KEGG; ecs:ECs0049; -.
DR   PATRIC; fig|386585.9.peg.148; -.
DR   eggNOG; ENOG4108MXQ; Bacteria.
DR   eggNOG; COG2249; LUCA.
DR   HOGENOM; HOG000149972; -.
DR   KO; K11746; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR   Gene3D; 3.40.50.360; -; 1.
DR   HAMAP; MF_01414; K_H_efflux_KefF; 1.
DR   InterPro; IPR003680; Flavodoxin_fold.
DR   InterPro; IPR029039; Flavoprotein-like_dom.
DR   InterPro; IPR023948; K_H_efflux_KefF.
DR   PANTHER; PTHR10204:SF50; PTHR10204:SF50; 1.
DR   Pfam; PF02525; Flavodoxin_2; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8XA24.
DR   SWISS-2DPAGE; Q8XA24.
KW   Cell inner membrane; Cell membrane; Complete proteome; Flavoprotein;
KW   FMN; Membrane; NAD; Oxidoreductase.
FT   CHAIN         1    176       Glutathione-regulated potassium-efflux
FT                                system ancillary protein KefF.
FT                                /FTId=PRO_0000071636.
FT   NP_BIND      14     17       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
FT   NP_BIND      65     68       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
FT   NP_BIND     105    108       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
FT   BINDING       8      8       FMN. {ECO:0000255|HAMAP-Rule:MF_01414}.
SQ   SEQUENCE   176 AA;  20204 MW;  F64FED71610EBCEB CRC64;
     MILIIYAHPY PHHSHANKRM FEQARTLEGV EIRSLYQLYP DFNIDIAAEQ EALSRADLIV
     WQHPMQWYSI PPLLKLWIDK VFSHGWAYGH GGTALHGKHL LWAVTTGGGE SHFEIGAHPG
     FDVLSQPLQA TAIYCGLNWL PPFAMHCTFI CDDETLEGQA RHYKQRLLEW QEAHHG
//

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