(data stored in ACNUC7421 zone)

SWISSPROT: RLUA_ECO57

ID   RLUA_ECO57              Reviewed;         219 AA.
AC   Q8XA10;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   05-JUL-2017, entry version 87.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase A;
DE            EC=5.4.99.28;
DE            EC=5.4.99.29;
DE   AltName: Full=23S rRNA pseudouridine(746) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase A;
DE   AltName: Full=rRNA-uridine isomerase A;
DE   AltName: Full=tRNA pseudouridine(32) synthase;
GN   Name=rluA; OrderedLocusNames=Z0066, ECs0062;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC       746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem
CC       and loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(32) = tRNA pseudouridine(32).
CC   -!- CATALYTIC ACTIVITY: 23S rRNA uridine(746) = 23S rRNA
CC       pseudouridine(746).
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
DR   EMBL; AE005174; AAG54362.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33485.1; -; Genomic_DNA.
DR   PIR; F85487; F85487.
DR   PIR; F90636; F90636.
DR   RefSeq; NP_308089.1; NC_002695.1.
DR   RefSeq; WP_000525186.1; NZ_MWVM01000003.1.
DR   ProteinModelPortal; Q8XA10; -.
DR   SMR; Q8XA10; -.
DR   STRING; 155864.Z0066; -.
DR   EnsemblBacteria; AAG54362; AAG54362; Z0066.
DR   EnsemblBacteria; BAB33485; BAB33485; BAB33485.
DR   GeneID; 913462; -.
DR   KEGG; ece:Z0066; -.
DR   KEGG; ecs:ECs0062; -.
DR   PATRIC; fig|386585.9.peg.161; -.
DR   eggNOG; ENOG4105D3G; Bacteria.
DR   eggNOG; COG0564; LUCA.
DR   HOGENOM; HOG000275919; -.
DR   KO; K06177; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluD.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8XA10.
DR   SWISS-2DPAGE; Q8XA10.
KW   Complete proteome; Isomerase; rRNA processing; tRNA processing.
FT   INIT_MET      1      1       Removed. {ECO:0000250}.
FT   CHAIN         2    219       Ribosomal large subunit pseudouridine
FT                                synthase A.
FT                                /FTId=PRO_0000162653.
FT   ACT_SITE     64     64       {ECO:0000250}.
SQ   SEQUENCE   219 AA;  24861 MW;  249A8F1279D8DD6E CRC64;
     MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI QRDYPQAESV
     HRLDMATSGV IVVALTKAAE RELKRQFRER VPKKQYVTRV WGHPSPAEGL VDLPLICDWP
     NRPKQKVCYE TGKPAQTEYE VVEYAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF
     YASPEARAMA PRLLLHAEML TITHPAYGNS MTFKAPADF
//

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