(data stored in ACNUC7421 zone)

SWISSPROT: SPEE_ECO57

ID   SPEE_ECO57              Reviewed;         288 AA.
AC   P66834; Q8X951;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   30-AUG-2017, entry version 88.
DE   RecName: Full=Polyamine aminopropyltransferase {ECO:0000255|HAMAP-Rule:MF_00198};
DE   AltName: Full=Putrescine aminopropyltransferase {ECO:0000255|HAMAP-Rule:MF_00198};
DE            Short=PAPT {ECO:0000255|HAMAP-Rule:MF_00198};
DE   AltName: Full=Spermidine synthase {ECO:0000255|HAMAP-Rule:MF_00198};
DE            Short=SPDS {ECO:0000255|HAMAP-Rule:MF_00198};
DE            Short=SPDSY {ECO:0000255|HAMAP-Rule:MF_00198};
DE            EC=2.5.1.16 {ECO:0000255|HAMAP-Rule:MF_00198};
GN   Name=speE {ECO:0000255|HAMAP-Rule:MF_00198};
GN   OrderedLocusNames=Z0131, ECs0125;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the irreversible transfer of a propylamine
CC       group from the amino donor S-adenosylmethioninamine (decarboxy-
CC       AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.
CC       {ECO:0000255|HAMAP-Rule:MF_00198}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl 3-(methylthio)propylamine +
CC       putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.
CC       {ECO:0000255|HAMAP-Rule:MF_00198}.
CC   -!- PATHWAY: Amine and polyamine biosynthesis; spermidine
CC       biosynthesis; spermidine from putrescine: step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_00198}.
CC   -!- SUBUNIT: Homodimer or homotetramer. {ECO:0000255|HAMAP-
CC       Rule:MF_00198}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00198}.
CC   -!- SIMILARITY: Belongs to the spermidine/spermine synthase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00198}.
DR   EMBL; AE005174; AAG54425.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33548.1; -; Genomic_DNA.
DR   PIR; E85495; E85495.
DR   PIR; E90644; E90644.
DR   RefSeq; NP_308152.1; NC_002695.1.
DR   RefSeq; WP_000818414.1; NZ_MWVM01000012.1.
DR   ProteinModelPortal; P66834; -.
DR   SMR; P66834; -.
DR   STRING; 155864.Z0131; -.
DR   EnsemblBacteria; AAG54425; AAG54425; Z0131.
DR   EnsemblBacteria; BAB33548; BAB33548; BAB33548.
DR   GeneID; 913703; -.
DR   KEGG; ece:Z0131; -.
DR   KEGG; ecs:ECs0125; -.
DR   PATRIC; fig|386585.9.peg.223; -.
DR   eggNOG; ENOG4105CCX; Bacteria.
DR   eggNOG; COG0421; LUCA.
DR   HOGENOM; HOG000256146; -.
DR   KO; K00797; -.
DR   UniPathway; UPA00248; UER00314.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004766; F:spermidine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008295; P:spermidine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.140.10; -; 1.
DR   HAMAP; MF_00198; Spermidine_synth; 1.
DR   InterPro; IPR030374; PABS.
DR   InterPro; IPR030373; PABS_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   InterPro; IPR001045; Spermi_synthase.
DR   InterPro; IPR035246; Spermidine_synt_N.
DR   Pfam; PF17284; Spermine_synt_N; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00417; speE; 1.
DR   PROSITE; PS01330; PABS_1; 1.
DR   PROSITE; PS51006; PABS_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; P66834.
DR   SWISS-2DPAGE; P66834.
KW   Complete proteome; Cytoplasm; Polyamine biosynthesis;
KW   Spermidine biosynthesis; Transferase.
FT   INIT_MET      1      1       Removed. {ECO:0000250}.
FT   CHAIN         2    288       Polyamine aminopropyltransferase.
FT                                /FTId=PRO_0000156481.
FT   DOMAIN        9    238       PABS. {ECO:0000255|HAMAP-Rule:MF_00198}.
FT   REGION       53     54       Polyamine binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00198}.
FT   REGION      140    141       S-adenosylmethioninamine binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_00198}.
FT   REGION      159    161       Polyamine binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00198}.
FT   ACT_SITE    158    158       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_00198}.
FT   BINDING       9      9       Polyamine. {ECO:0000255|HAMAP-
FT                                Rule:MF_00198}.
FT   BINDING      33     33       S-adenosylmethioninamine.
FT                                {ECO:0000255|HAMAP-Rule:MF_00198}.
FT   BINDING      64     64       Polyamine. {ECO:0000255|HAMAP-
FT                                Rule:MF_00198}.
FT   BINDING      88     88       Polyamine. {ECO:0000255|HAMAP-
FT                                Rule:MF_00198}.
FT   BINDING     108    108       S-adenosylmethioninamine.
FT                                {ECO:0000255|HAMAP-Rule:MF_00198}.
FT   BINDING     165    165       S-adenosylmethioninamine; via carbonyl
FT                                oxygen. {ECO:0000255|HAMAP-
FT                                Rule:MF_00198}.
SQ   SEQUENCE   288 AA;  32307 MW;  31AE026FE0754E9F CRC64;
     MAEKKQWHET LHDQFGQYFA VDNVLYHEKT DHQDLIIFEN AAFGRVMALD GVVQTTERDE
     FIYHEMMTHV PLLAHGHAKH VLIIGGGDGA MLREVTRHKN VESITMVEID AGVVSFCRQY
     LPNHNAGSYD DPRFKLVIDD GVNFVNQTSQ TFDVIISDCT DPIGPGESLF TSAFYEGCKR
     CLNPGGIFVA QNGVCFLQQE EAIDSHRKLS HYFSDVGFYQ AAIPTYYGGI MTFAWATDND
     ALRHLSTEII QARFLASGLK CRYYNPAVHT AAFALPQYLQ DALASQPS
//

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