(data stored in ACNUC7421 zone)

SWISSPROT: LPXB_ECO57

ID   LPXB_ECO57              Reviewed;         382 AA.
AC   Q8X8X7;
DT   27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   05-JUL-2017, entry version 84.
DE   RecName: Full=Lipid-A-disaccharide synthase {ECO:0000255|HAMAP-Rule:MF_00392};
DE            EC=2.4.1.182 {ECO:0000255|HAMAP-Rule:MF_00392};
GN   Name=lpxB {ECO:0000255|HAMAP-Rule:MF_00392};
GN   OrderedLocusNames=Z0194, ECs0184;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC       diacylglucosamine-1-phosphate to form lipid A disaccharide, a
CC       precursor of lipid A, a phosphorylated glycolipid that anchors the
CC       lipopolysaccharide to the outer membrane of the cell.
CC       {ECO:0000255|HAMAP-Rule:MF_00392}.
CC   -!- CATALYTIC ACTIVITY: UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-
CC       alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-
CC       alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-
CC       hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-
CC       3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_00392}.
CC   -!- PATHWAY: Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid
CC       IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and
CC       UDP-N-acetyl-alpha-D-glucosamine: step 5/6. {ECO:0000255|HAMAP-
CC       Rule:MF_00392}.
CC   -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00392}.
DR   EMBL; AE005174; AAG54484.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33607.1; -; Genomic_DNA.
DR   PIR; H85502; H85502.
DR   PIR; H90651; H90651.
DR   RefSeq; NP_308211.1; NC_002695.1.
DR   RefSeq; WP_000139661.1; NZ_MWVM01000012.1.
DR   ProteinModelPortal; Q8X8X7; -.
DR   STRING; 155864.Z0194; -.
DR   CAZy; GT19; Glycosyltransferase Family 19.
DR   EnsemblBacteria; AAG54484; AAG54484; Z0194.
DR   EnsemblBacteria; BAB33607; BAB33607; BAB33607.
DR   GeneID; 913894; -.
DR   KEGG; ece:Z0194; -.
DR   KEGG; ecs:ECs0184; -.
DR   PATRIC; fig|386585.9.peg.287; -.
DR   eggNOG; ENOG4105D0V; Bacteria.
DR   eggNOG; COG0763; LUCA.
DR   HOGENOM; HOG000018003; -.
DR   KO; K00748; -.
DR   UniPathway; UPA00359; UER00481.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00392; LpxB; 1.
DR   InterPro; IPR003835; Glyco_trans_19.
DR   PANTHER; PTHR30372; PTHR30372; 1.
DR   Pfam; PF02684; LpxB; 1.
DR   TIGRFAMs; TIGR00215; lpxB; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8X8X7.
DR   SWISS-2DPAGE; Q8X8X7.
KW   Complete proteome; Glycosyltransferase; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Transferase.
FT   CHAIN         1    382       Lipid-A-disaccharide synthase.
FT                                /FTId=PRO_0000190166.
SQ   SEQUENCE   382 AA;  42398 MW;  6B2D0F2C8FE32347 CRC64;
     MTEQRPLTIA LVAGETSGDI LGAGLIRALK ERVPNARFVG VAGPRMQAEG CEAWYEMEEL
     AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP DFNIILEGNL KKQGIKTIHY
     VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE KAFYDKYNVP CRFIGHTMAD AMPLDPDKNS
     ARDVLGIPYD AHCLALLPGS RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ
     FERIKAAVAP DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF
     WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT SHAMHDTFRE
     LHQQIRCNAD EQAAQAVLEL AQ
//

If you have problems or comments...

PBIL Back to PBIL home page