(data stored in ACNUC7421 zone)

SWISSPROT: RNH_ECO57

ID   RNH_ECO57               Reviewed;         155 AA.
AC   P0A7Y6; P00647; Q8FKY5;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   08-MAY-2019, entry version 89.
DE   RecName: Full=Ribonuclease HI;
DE            Short=RNase HI;
DE            EC=3.1.26.4;
GN   Name=rnhA; OrderedLocusNames=Z0239, ECs0210;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Endonuclease that specifically degrades the RNA of RNA-
CC       DNA hybrids. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.;
CC         EC=3.1.26.4;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. May bind a second metal ion
CC       at a regulatory site, or after substrate binding. {ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the RNase H family. {ECO:0000305}.
DR   EMBL; AE005174; AAG54510.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33633.1; -; Genomic_DNA.
DR   PIR; B85506; B85506.
DR   PIR; B90655; B90655.
DR   RefSeq; NP_308237.1; NC_002695.1.
DR   RefSeq; WP_000917883.1; NZ_SDVX01000001.1.
DR   SMR; P0A7Y6; -.
DR   STRING; 155864.EDL933_0214; -.
DR   EnsemblBacteria; AAG54510; AAG54510; Z0239.
DR   EnsemblBacteria; BAB33633; BAB33633; BAB33633.
DR   GeneID; 914053; -.
DR   KEGG; ece:Z0239; -.
DR   KEGG; ecs:ECs0210; -.
DR   PATRIC; fig|386585.9.peg.314; -.
DR   eggNOG; ENOG4108UMW; Bacteria.
DR   eggNOG; COG0328; LUCA.
DR   HOGENOM; HOG000040465; -.
DR   KO; K03469; -.
DR   BioCyc; ECOO157:RNHA-MONOMER; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd09278; RNase_HI_prokaryote_like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00042; RNase_H; 1.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR002156; RNaseH_domain.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR022892; RNaseHI.
DR   Pfam; PF00075; RNase_H; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50879; RNASE_H; 1.
PE   3: Inferred from homology;
DR   PRODOM; P0A7Y6.
DR   SWISS-2DPAGE; P0A7Y6.
KW   Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Magnesium;
KW   Metal-binding; Nuclease; Reference proteome.
FT   CHAIN         1    155       Ribonuclease HI.
FT                                /FTId=PRO_0000195373.
FT   DOMAIN        1    142       RNase H.
FT   METAL        10     10       Magnesium 1. {ECO:0000250}.
FT   METAL        10     10       Magnesium 2. {ECO:0000250}.
FT   METAL        48     48       Magnesium 1. {ECO:0000250}.
FT   METAL        70     70       Magnesium 1. {ECO:0000250}.
FT   METAL       134    134       Magnesium 2. {ECO:0000250}.
SQ   SEQUENCE   155 AA;  17597 MW;  B8EF81EEB2F94CBE CRC64;
     MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK
     EHCEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW
     VKGHAGHPEN ERCDELARAA AMNPTLEDTG YQVEV
//

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