(data stored in ACNUC7421 zone)

SWISSPROT: BETA_ECO57

ID   BETA_ECO57              Reviewed;         562 AA.
AC   Q8X6C6;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   07-JUN-2017, entry version 104.
DE   RecName: Full=Oxygen-dependent choline dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00750};
DE            Short=CDH {ECO:0000255|HAMAP-Rule:MF_00750};
DE            Short=CHD {ECO:0000255|HAMAP-Rule:MF_00750};
DE            EC=1.1.99.1 {ECO:0000255|HAMAP-Rule:MF_00750};
DE   AltName: Full=Betaine aldehyde dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00750};
DE            Short=BADH {ECO:0000255|HAMAP-Rule:MF_00750};
DE            EC=1.2.1.8 {ECO:0000255|HAMAP-Rule:MF_00750};
GN   Name=betA {ECO:0000255|HAMAP-Rule:MF_00750};
GN   OrderedLocusNames=Z0398, ECs0357;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Involved in the biosynthesis of the osmoprotectant
CC       glycine betaine. Catalyzes the oxidation of choline to betaine
CC       aldehyde and betaine aldehyde to glycine betaine at the same rate.
CC       {ECO:0000255|HAMAP-Rule:MF_00750}.
CC   -!- CATALYTIC ACTIVITY: Choline + acceptor = betaine aldehyde +
CC       reduced acceptor. {ECO:0000255|HAMAP-Rule:MF_00750}.
CC   -!- CATALYTIC ACTIVITY: Betaine aldehyde + NAD(+) + H(2)O = betaine +
CC       NADH. {ECO:0000255|HAMAP-Rule:MF_00750}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00750};
CC   -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis
CC       via choline pathway; betaine aldehyde from choline (cytochrome c
CC       reductase route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00750}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00750}.
DR   EMBL; AE005174; AAG54653.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33780.1; -; Genomic_DNA.
DR   PIR; A85524; A85524.
DR   PIR; E90673; E90673.
DR   RefSeq; NP_308384.1; NC_002695.1.
DR   RefSeq; WP_001159114.1; NZ_LPWC02000002.1.
DR   ProteinModelPortal; Q8X6C6; -.
DR   SMR; Q8X6C6; -.
DR   STRING; 155864.Z0398; -.
DR   EnsemblBacteria; AAG54653; AAG54653; Z0398.
DR   EnsemblBacteria; BAB33780; BAB33780; BAB33780.
DR   GeneID; 914458; -.
DR   KEGG; ece:Z0398; -.
DR   KEGG; ecs:ECs0357; -.
DR   PATRIC; fig|386585.9.peg.449; -.
DR   eggNOG; ENOG4105CZ6; Bacteria.
DR   eggNOG; COG2303; LUCA.
DR   HOGENOM; HOG000139600; -.
DR   KO; K00108; -.
DR   UniPathway; UPA00529; UER00385.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008802; F:betaine-aldehyde dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008812; F:choline dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0019285; P:glycine betaine biosynthetic process from choline; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00750; Choline_dehydrogen; 1.
DR   InterPro; IPR011533; BetA.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR012132; GMC_OxRdtase.
DR   InterPro; IPR000172; GMC_OxRdtase_N.
DR   InterPro; IPR007867; GMC_OxRtase_C.
DR   PANTHER; PTHR11552:SF159; PTHR11552:SF159; 1.
DR   Pfam; PF05199; GMC_oxred_C; 1.
DR   Pfam; PF00732; GMC_oxred_N; 1.
DR   PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 2.
DR   TIGRFAMs; TIGR01810; betA; 1.
DR   PROSITE; PS00623; GMC_OXRED_1; 1.
DR   PROSITE; PS00624; GMC_OXRED_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8X6C6.
DR   SWISS-2DPAGE; Q8X6C6.
KW   Cell membrane; Complete proteome; FAD; Flavoprotein; Membrane; NAD;
KW   Oxidoreductase.
FT   CHAIN         1    562       Oxygen-dependent choline dehydrogenase.
FT                                /FTId=PRO_0000205587.
FT   NP_BIND       4     33       FAD. {ECO:0000255|HAMAP-Rule:MF_00750}.
FT   ACT_SITE    473    473       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_00750}.
SQ   SEQUENCE   562 AA;  62633 MW;  7DC9B36EC06D24ED CRC64;
     MQFDYIIIGA GSAGNVLATR LTEDPNTSVL LLEAGGPDYR FDFRTQMPAA LAFPLQGKRY
     NWAYETEPEP FMNNRRMECG RGKGLGGSSL INGMCYIRGN AMDLDNWAKE PGLENWSYLD
     CLPYYRKAET RDVGENDYHG GDGPVSVTTS KPGVNPLFEA MIEAGVQAGY PRTDDLNGYQ
     QEGFGPMDRT VTPQGRRAST ARGYLDQAKS RPNLTIRTHA MTDHIIFDCK RAVGVEWLEG
     DSTIPTRATA NKEVLLCAGA IASPQILQRS GVGNAELLAE FDIPLVHDLP GVGENLQDHL
     EMYLQYECKE PVSLYPALQW WNQPKIGAEW LFGGTGVGAS NHFEAGGFIR SREEFAWPNI
     QYHFLPVAIN YNGSNAVKEH GFQCHVGSMR SPSRGHVRIK SRDPHQHPAI LFNYMSHEQD
     WQEFRDAIRI TREIMHQPAL DQYRGREISP GTECQTDEQL DEFVRNHAET AFHPCGTCKM
     GYDEMSVVDG EGRVHGLEGL RVVDASIMPQ IITGNLNATT IMIGEKMADM IRGKEALPRS
     TAGYFVANGM PVRAKKMSRD LN
//

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