(data stored in ACNUC7421 zone)

SWISSPROT: CYNS_ECO57

ID   CYNS_ECO57              Reviewed;         156 AA.
AC   P58704;
DT   31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2002, sequence version 1.
DT   05-JUL-2017, entry version 94.
DE   RecName: Full=Cyanate hydratase;
DE            Short=Cyanase;
DE            EC=4.2.1.104;
DE   AltName: Full=Cyanate hydrolase;
DE   AltName: Full=Cyanate lyase;
GN   Name=cynS; Synonyms=cnt; OrderedLocusNames=Z0436, ECs0393;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to
CC       produce ammonia and carbon dioxide. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY: Cyanate + HCO(3)(-) + 2 H(+) = NH(3) + 2
CC       CO(2).
CC   -!- SUBUNIT: Homodecamer composed of five homodimers. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000305}.
DR   EMBL; AE005174; AAG54689.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33816.1; -; Genomic_DNA.
DR   PIR; A99678; A99678.
DR   PIR; E85528; E85528.
DR   RefSeq; NP_308420.1; NC_002695.1.
DR   RefSeq; WP_000616247.1; NZ_MWVM01000022.1.
DR   ProteinModelPortal; P58704; -.
DR   SMR; P58704; -.
DR   STRING; 155864.Z0436; -.
DR   EnsemblBacteria; AAG54689; AAG54689; Z0436.
DR   EnsemblBacteria; BAB33816; BAB33816; BAB33816.
DR   GeneID; 914495; -.
DR   KEGG; ece:Z0436; -.
DR   KEGG; ecs:ECs0393; -.
DR   PATRIC; fig|386585.9.peg.488; -.
DR   eggNOG; ENOG4108V7B; Bacteria.
DR   eggNOG; COG1513; LUCA.
DR   HOGENOM; HOG000043436; -.
DR   KO; K01725; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009439; P:cyanate metabolic process; IEA:InterPro.
DR   CDD; cd00559; Cyanase_C; 1.
DR   Gene3D; 3.30.1160.10; -; 1.
DR   HAMAP; MF_00535; Cyanate_hydrat; 1.
DR   InterPro; IPR008076; Cyanase.
DR   InterPro; IPR003712; Cyanate_lyase_C.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom.
DR   PANTHER; PTHR34186; PTHR34186; 1.
DR   Pfam; PF02560; Cyanate_lyase; 1.
DR   PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR   PRINTS; PR01693; CYANASE.
DR   SMART; SM01116; Cyanate_lyase; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF55234; SSF55234; 1.
DR   TIGRFAMs; TIGR00673; cynS; 1.
PE   3: Inferred from homology;
DR   PRODOM; P58704.
DR   SWISS-2DPAGE; P58704.
KW   Complete proteome; Lyase.
FT   CHAIN         1    156       Cyanate hydratase.
FT                                /FTId=PRO_0000187524.
FT   ACT_SITE     96     96       {ECO:0000250}.
FT   ACT_SITE     99     99       {ECO:0000250}.
FT   ACT_SITE    122    122       {ECO:0000250}.
SQ   SEQUENCE   156 AA;  17048 MW;  926070D528CFDDFC CRC64;
     MIQSQINRNI RLDLADAILL SKAKKDLSFA EIADGTGLAE AFVTAALLGQ QALPADAARQ
     VGAKLDLDED AILLLQMIPL RGCIDDRIPT DPTMYRFYEM LQVYGTTLKA LVHEKFGDGI
     ISAINFKLDV KKVADPEGGE RAVITLDGKY LPTKPF
//

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