(data stored in ACNUC7421 zone)

SWISSPROT: MHPB_ECO57

ID   MHPB_ECO57              Reviewed;         314 AA.
AC   P0ABS0; P54711; P77048; P77461;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   05-JUL-2017, entry version 74.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase {ECO:0000255|HAMAP-Rule:MF_01653};
DE            EC=1.13.11.16 {ECO:0000255|HAMAP-Rule:MF_01653};
DE   AltName: Full=3-carboxyethylcatechol 2,3-dioxygenase {ECO:0000255|HAMAP-Rule:MF_01653};
GN   Name=mhpB {ECO:0000255|HAMAP-Rule:MF_01653};
GN   OrderedLocusNames=Z0446, ECs0403;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative
CC       cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-
CC       dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-
CC       hydroxy-6-ketononatrienedioate, respectively. {ECO:0000255|HAMAP-
CC       Rule:MF_01653}.
CC   -!- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O(2) =
CC       (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate.
CC       {ECO:0000255|HAMAP-Rule:MF_01653}.
CC   -!- CATALYTIC ACTIVITY: (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate +
CC       O(2) = (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.
CC       {ECO:0000255|HAMAP-Rule:MF_01653}.
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01653};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate
CC       degradation. {ECO:0000255|HAMAP-Rule:MF_01653}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01653}.
CC   -!- SIMILARITY: Belongs to the LigB/MhpB extradiol dioxygenase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01653}.
DR   EMBL; AE005174; AAG54699.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33826.1; -; Genomic_DNA.
DR   PIR; C90679; C90679.
DR   PIR; G85529; G85529.
DR   RefSeq; NP_308430.1; NC_002695.1.
DR   RefSeq; WP_000543457.1; NZ_MWVM01000022.1.
DR   ProteinModelPortal; P0ABS0; -.
DR   STRING; 155864.Z0446; -.
DR   EnsemblBacteria; AAG54699; AAG54699; Z0446.
DR   EnsemblBacteria; BAB33826; BAB33826; BAB33826.
DR   GeneID; 914505; -.
DR   KEGG; ece:Z0446; -.
DR   KEGG; ecs:ECs0403; -.
DR   PATRIC; fig|386585.9.peg.498; -.
DR   eggNOG; ENOG4105MI0; Bacteria.
DR   eggNOG; ENOG410XPPS; LUCA.
DR   HOGENOM; HOG000069851; -.
DR   KO; K05713; -.
DR   UniPathway; UPA00714; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd07365; MhpB_like; 1.
DR   HAMAP; MF_01653; MhpB; 1.
DR   InterPro; IPR023789; DHPP/DHXA_dioxygenase.
DR   InterPro; IPR004183; Xdiol_dOase_suB.
DR   Pfam; PF02900; LigB; 1.
PE   3: Inferred from homology;
DR   PRODOM; P0ABS0.
DR   SWISS-2DPAGE; P0ABS0.
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase;
KW   Iron; Oxidoreductase.
FT   CHAIN         1    314       2,3-dihydroxyphenylpropionate/2,3-
FT                                dihydroxicinnamic acid 1,2-dioxygenase.
FT                                /FTId=PRO_0000085104.
FT   ACT_SITE    115    115       Proton donor. {ECO:0000255|HAMAP-
FT                                Rule:MF_01653}.
FT   ACT_SITE    179    179       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_01653}.
SQ   SEQUENCE   314 AA;  34196 MW;  E1D5A8574E5DFE05 CRC64;
     MHAYLHCLSH SPLVGYVDPA QEVLDEVNGV IASARERIAA FSPELVVLFA PDHYNGFFYD
     VMPPFCLGVG ATAIGDFGSA AGELPVPVEL AEACAHAVMK SGIDLAVSYC MQVDHGFAQP
     LEFLLGGLDK VPVLPVFING VATPLPGFQR TRMLGEAIGR FTSTLNKRVL FLGSGGLSHQ
     PPVPELAKAD AHMRDRLLGS GKDLPASERE LRQQRVISAA EKFVEDQRTL HPLNPIWDNQ
     FMTLLEQGRI QELDAVSNEE LSAIAGKSTH EIKTWVAAFA AISAFGNWRS EGRYYRPIPE
     WIAGFGSLSA RTEN
//

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