(data stored in ACNUC7421 zone)

SWISSPROT: MHPD_ECO57

ID   MHPD_ECO57              Reviewed;         269 AA.
AC   Q8XEC1; Q7AH51;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   05-JUL-2017, entry version 85.
DE   RecName: Full=2-keto-4-pentenoate hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
DE            EC=4.2.1.80 {ECO:0000255|HAMAP-Rule:MF_01655};
DE   AltName: Full=2-hydroxypentadienoic acid hydratase {ECO:0000255|HAMAP-Rule:MF_01655};
GN   Name=mhpD {ECO:0000255|HAMAP-Rule:MF_01655};
GN   OrderedLocusNames=Z0448, ECs0405;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxypentadienoic acid
CC       (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic
CC       acid. {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- CATALYTIC ACTIVITY: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate
CC       + H(2)O. {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01655};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate
CC       degradation. {ECO:0000255|HAMAP-Rule:MF_01655}.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family. MhpD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01655}.
DR   EMBL; AE005174; AAG54701.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33828.1; -; Genomic_DNA.
DR   PIR; A85530; A85530.
DR   PIR; E90679; E90679.
DR   RefSeq; NP_308432.1; NC_002695.1.
DR   RefSeq; WP_000160723.1; NZ_MWVM01000022.1.
DR   ProteinModelPortal; Q8XEC1; -.
DR   SMR; Q8XEC1; -.
DR   STRING; 155864.Z0448; -.
DR   EnsemblBacteria; AAG54701; AAG54701; Z0448.
DR   EnsemblBacteria; BAB33828; BAB33828; BAB33828.
DR   GeneID; 914507; -.
DR   KEGG; ece:Z0448; -.
DR   KEGG; ecs:ECs0405; -.
DR   PATRIC; fig|386585.9.peg.500; -.
DR   eggNOG; ENOG4105IKQ; Bacteria.
DR   eggNOG; COG3971; LUCA.
DR   HOGENOM; HOG000179637; -.
DR   KO; K02554; -.
DR   UniPathway; UPA00714; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0008684; F:2-oxopent-4-enoate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   HAMAP; MF_01655; MhpD; 1.
DR   InterPro; IPR011234; Fumarylacetoacetase_C-rel.
DR   InterPro; IPR023793; Keto_pentenoate-hydratase.
DR   Pfam; PF01557; FAA_hydrolase; 1.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8XEC1.
DR   SWISS-2DPAGE; Q8XEC1.
KW   Aromatic hydrocarbons catabolism; Complete proteome; Lyase.
FT   CHAIN         1    269       2-keto-4-pentenoate hydratase.
FT                                /FTId=PRO_0000337794.
FT   CONFLICT    167    167       G -> A (in Ref. 2; BAB33828).
FT                                {ECO:0000305}.
SQ   SEQUENCE   269 AA;  28856 MW;  C147BF601093C942 CRC64;
     MTKHTLEQLA ADLRRAAEQG EAIAPLRDLI GIDNAEAAYA IQHINVQYDV AQGRRVVGRK
     VGLTHPKVQQ QLGVDQPDFG TLFADMCYGD NEIIPFSRVL QPRIEAEIAL VLNRDLPATD
     ITFDELYNAI EWVLPALEVV GSRIRDWSIQ FVDTVADNAS CGVYVIGGPA QRPAGLDLKN
     CAMKMTRNNE EVSSGRGSEC LGHPLNAAVW LARKMASLGE PLRAGDIILT GALGPMVAVN
     AGDRFEAHIE GIGSVAAAFS SAAPKGSLS
//

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