(data stored in ACNUC7421 zone)

SWISSPROT: QUEA_ECO57

ID   QUEA_ECO57              Reviewed;         356 AA.
AC   P0A7G0; P21516;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   05-JUL-2017, entry version 72.
DE   RecName: Full=S-adenosylmethionine:tRNA ribosyltransferase-isomerase {ECO:0000255|HAMAP-Rule:MF_00113};
DE            EC=2.4.99.17 {ECO:0000255|HAMAP-Rule:MF_00113};
DE   AltName: Full=Queuosine biosynthesis protein QueA {ECO:0000255|HAMAP-Rule:MF_00113};
GN   Name=queA {ECO:0000255|HAMAP-Rule:MF_00113};
GN   OrderedLocusNames=Z0504, ECs0456;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet
CC       to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give
CC       epoxyqueuosine (oQ-tRNA). {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 7-aminomethyl-7-
CC       carbaguanosine(34) in tRNA = L-methionine + adenine +
CC       epoxyqueuosine(34) in tRNA. {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SIMILARITY: Belongs to the QueA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00113}.
DR   EMBL; AE005174; AAG54752.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33879.1; -; Genomic_DNA.
DR   PIR; D85536; D85536.
DR   PIR; H90685; H90685.
DR   RefSeq; NP_308483.1; NC_002695.1.
DR   RefSeq; WP_001266503.1; NZ_MWVM01000009.1.
DR   ProteinModelPortal; P0A7G0; -.
DR   SMR; P0A7G0; -.
DR   STRING; 155864.Z0504; -.
DR   EnsemblBacteria; AAG54752; AAG54752; Z0504.
DR   EnsemblBacteria; BAB33879; BAB33879; BAB33879.
DR   GeneID; 914558; -.
DR   KEGG; ece:Z0504; -.
DR   KEGG; ecs:ECs0456; -.
DR   PATRIC; fig|386585.9.peg.556; -.
DR   eggNOG; ENOG4105E2N; Bacteria.
DR   eggNOG; COG0809; LUCA.
DR   HOGENOM; HOG000004401; -.
DR   KO; K07568; -.
DR   UniPathway; UPA00392; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016853; F:isomerase activity; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00113; QueA; 1.
DR   InterPro; IPR003699; QueA.
DR   Pfam; PF02547; Queuosine_synth; 1.
DR   SUPFAM; SSF111337; SSF111337; 1.
DR   TIGRFAMs; TIGR00113; queA; 1.
PE   3: Inferred from homology;
DR   PRODOM; P0A7G0.
DR   SWISS-2DPAGE; P0A7G0.
KW   Complete proteome; Cytoplasm; Queuosine biosynthesis;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    356       S-adenosylmethionine:tRNA
FT                                ribosyltransferase-isomerase.
FT                                /FTId=PRO_0000165401.
SQ   SEQUENCE   356 AA;  39431 MW;  47F7F0090812DCF3 CRC64;
     MRVTDFSFEL PESLIAHYPM PERSSCRLLS LDGPTGALTH GTFTDLLDKL NPGDLLVFNN
     TRVIPARLFG RKASGGKIEV LVERMLDDKR ILAHIRASKA PKPGAELLLG DDESINATMT
     ARHGALFEVE FNDERSVLDI LNSIGHMPLP PYIDRPDEDA DRELYQTVYS EKPGAVAAPT
     AGLHFDEPLL EKLRAKGVEM AFVTLHVGAG TFQPVRVDTI EDHIMHSEYA EVPQDVVDAV
     LAAKARGNRV IAVGTTSVRS LESAAQAAKN DLIEPFFDDT QIFIYPGFQY KVVDALVTNF
     HLPESTLIML VSAFAGYQHT MNAYKAAVEE KYRFFSYGDA MFITYNPQAI NERVGE
//

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