(data stored in ACNUC7421 zone)

SWISSPROT: AMTB_ECO57

ID   AMTB_ECO57              Reviewed;         428 AA.
AC   P69680; P37905;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 1.
DT   05-JUL-2017, entry version 81.
DE   RecName: Full=Ammonia channel;
DE   AltName: Full=Ammonia transporter;
DE   Flags: Precursor;
GN   Name=amtB; OrderedLocusNames=Z0563, ECs0505;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Involved in the uptake of ammonia. {ECO:0000250}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-
CC       pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ammonia transporter channel
CC       (TC 1.A.11.2) family. {ECO:0000305}.
DR   EMBL; AE005174; AAG54801.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33928.1; -; Genomic_DNA.
DR   PIR; C64775; C64775.
DR   PIR; E85542; E85542.
DR   RefSeq; NP_308532.1; NC_002695.1.
DR   RefSeq; WP_000685029.1; NZ_MWVM01000009.1.
DR   PDB; 4NH2; X-ray; 2.30 A; A/B/C/D/E/F=26-428.
DR   PDBsum; 4NH2; -.
DR   ProteinModelPortal; P69680; -.
DR   SMR; P69680; -.
DR   STRING; 155864.Z0563; -.
DR   EnsemblBacteria; AAG54801; AAG54801; Z0563.
DR   EnsemblBacteria; BAB33928; BAB33928; BAB33928.
DR   GeneID; 914608; -.
DR   KEGG; ece:Z0563; -.
DR   KEGG; ecs:ECs0505; -.
DR   PATRIC; fig|386585.9.peg.610; -.
DR   eggNOG; ENOG4105BZU; Bacteria.
DR   eggNOG; ENOG410XNMH; LUCA.
DR   HOGENOM; HOG000017736; -.
DR   KO; K03320; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR001905; Ammonium_transpt.
DR   InterPro; IPR018047; Ammonium_transpt_CS.
DR   InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR   Pfam; PF00909; Ammonium_transp; 1.
DR   SUPFAM; SSF111352; SSF111352; 1.
DR   TIGRFAMs; TIGR00836; amt; 1.
DR   PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P69680.
DR   SWISS-2DPAGE; P69680.
KW   3D-structure; Ammonia transport; Cell inner membrane; Cell membrane;
KW   Complete proteome; Membrane; Signal; Transmembrane;
KW   Transmembrane helix; Transport.
FT   SIGNAL        1     22       {ECO:0000250}.
FT   CHAIN        23    428       Ammonia channel.
FT                                /FTId=PRO_0000001308.
FT   TOPO_DOM     23     28       Periplasmic. {ECO:0000250}.
FT   TRANSMEM     29     55       Helical; Name=1. {ECO:0000250}.
FT   TOPO_DOM     56     59       Cytoplasmic. {ECO:0000250}.
FT   TRANSMEM     60     90       Helical; Name=2. {ECO:0000250}.
FT   TOPO_DOM     91    118       Periplasmic. {ECO:0000250}.
FT   TRANSMEM    119    141       Helical; Name=3. {ECO:0000250}.
FT   TOPO_DOM    142    146       Cytoplasmic. {ECO:0000250}.
FT   TRANSMEM    147    170       Helical; Name=4. {ECO:0000250}.
FT   TOPO_DOM    171    184       Periplasmic. {ECO:0000250}.
FT   TRANSMEM    185    203       Helical; Name=5. {ECO:0000250}.
FT   TOPO_DOM    204    215       Cytoplasmic. {ECO:0000250}.
FT   TRANSMEM    216    242       Helical; Name=6. {ECO:0000250}.
FT   TOPO_DOM    243    245       Periplasmic. {ECO:0000250}.
FT   TRANSMEM    246    275       Helical; Name=7. {ECO:0000250}.
FT   TOPO_DOM    276    279       Cytoplasmic. {ECO:0000250}.
FT   TRANSMEM    280    294       Helical; Name=8. {ECO:0000250}.
FT   TOPO_DOM    295    302       Periplasmic. {ECO:0000250}.
FT   TRANSMEM    303    328       Helical; Name=9. {ECO:0000250}.
FT   TOPO_DOM    329    332       Cytoplasmic. {ECO:0000250}.
FT   TRANSMEM    333    354       Helical; Name=10. {ECO:0000250}.
FT   TOPO_DOM    355    369       Periplasmic. {ECO:0000250}.
FT   TRANSMEM    370    402       Helical; Name=11. {ECO:0000250}.
FT   TOPO_DOM    403    428       Cytoplasmic. {ECO:0000250}.
FT   HELIX        29     46       {ECO:0000244|PDB:4NH2}.
FT   HELIX        49     55       {ECO:0000244|PDB:4NH2}.
FT   HELIX        60     62       {ECO:0000244|PDB:4NH2}.
FT   HELIX        63     83       {ECO:0000244|PDB:4NH2}.
FT   HELIX        85     90       {ECO:0000244|PDB:4NH2}.
FT   STRAND       94     96       {ECO:0000244|PDB:4NH2}.
FT   STRAND      101    103       {ECO:0000244|PDB:4NH2}.
FT   TURN        104    107       {ECO:0000244|PDB:4NH2}.
FT   HELIX       119    141       {ECO:0000244|PDB:4NH2}.
FT   HELIX       147    160       {ECO:0000244|PDB:4NH2}.
FT   HELIX       162    170       {ECO:0000244|PDB:4NH2}.
FT   STRAND      171    173       {ECO:0000244|PDB:4NH2}.
FT   HELIX       174    177       {ECO:0000244|PDB:4NH2}.
FT   TURN        186    189       {ECO:0000244|PDB:4NH2}.
FT   HELIX       190    202       {ECO:0000244|PDB:4NH2}.
FT   HELIX       220    239       {ECO:0000244|PDB:4NH2}.
FT   HELIX       240    242       {ECO:0000244|PDB:4NH2}.
FT   STRAND      243    246       {ECO:0000244|PDB:4NH2}.
FT   HELIX       247    275       {ECO:0000244|PDB:4NH2}.
FT   HELIX       280    294       {ECO:0000244|PDB:4NH2}.
FT   TURN        295    300       {ECO:0000244|PDB:4NH2}.
FT   HELIX       303    322       {ECO:0000244|PDB:4NH2}.
FT   HELIX       335    354       {ECO:0000244|PDB:4NH2}.
FT   HELIX       357    359       {ECO:0000244|PDB:4NH2}.
FT   HELIX       370    402       {ECO:0000244|PDB:4NH2}.
SQ   SEQUENCE   428 AA;  44515 MW;  B4AC96F0E5AE2B59 CRC64;
     MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI ALFYGGLIRG
     KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI NWLMLKNIEL TAVMGSIYQY
     IHVAFQGSFA CITVGLIVGA LAERIRFSAV LIFVVVWLTL SYIPIAHMVW GGGLLASHGA
     LDFAGGTVVH INAAIAGLVG AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG
     SAGTANEIAA LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY
     IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM TGIFAASSLG
     GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD LTVGLRVPEE QEREGLDVNS
     HGENAYNA
//

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