(data stored in ACNUC7421 zone)

SWISSPROT: ACRA_ECO57

ID   ACRA_ECO57              Reviewed;         397 AA.
AC   P0AE07; P31223;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   05-JUL-2017, entry version 78.
DE   RecName: Full=Multidrug efflux pump subunit AcrA;
DE   AltName: Full=AcrAB-TolC multidrug efflux pump subunit AcrA;
DE   AltName: Full=Acriflavine resistance protein A;
DE   Flags: Precursor;
GN   Name=acrA; OrderedLocusNames=Z0578, ECs0516;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: AcrA-AcrB-AcrZ-TolC is a drug efflux protein complex
CC       with broad substrate specificity that uses the proton motive force
CC       to export substrates. This subunit may act as an adapter protein
CC       that links AcrB and TolC stably together (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Monomeric in solution. Homotrimeric; interacts
CC       indpendently with AcrB and TolC as well as AcrZ. Part of the AcrA-
CC       AcrB-TolC efflux pump (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Lipid-
CC       anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP)
CC       (TC 8.A.1) family. {ECO:0000305}.
DR   EMBL; AE005174; AAG54812.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33939.1; -; Genomic_DNA.
DR   PIR; D90693; D90693.
DR   PIR; H85543; H85543.
DR   RefSeq; NP_308543.1; NC_002695.1.
DR   RefSeq; WP_001295324.1; NZ_MWVM01000009.1.
DR   ProteinModelPortal; P0AE07; -.
DR   SMR; P0AE07; -.
DR   STRING; 155864.Z0578; -.
DR   PRIDE; P0AE07; -.
DR   EnsemblBacteria; AAG54812; AAG54812; Z0578.
DR   EnsemblBacteria; BAB33939; BAB33939; BAB33939.
DR   GeneID; 914620; -.
DR   KEGG; ece:Z0578; -.
DR   KEGG; ecs:ECs0516; -.
DR   PATRIC; fig|386585.9.peg.621; -.
DR   eggNOG; ENOG4105C1P; Bacteria.
DR   eggNOG; ENOG410XNVN; LUCA.
DR   HOGENOM; HOG000158247; -.
DR   KO; K03585; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005215; F:transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; HlyD; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
DR   PRODOM; P0AE07.
DR   SWISS-2DPAGE; P0AE07.
KW   Antibiotic resistance; Cell inner membrane; Cell membrane;
KW   Coiled coil; Complete proteome; Lipoprotein; Membrane; Palmitate;
KW   Signal; Transport.
FT   SIGNAL        1     24       {ECO:0000255|PROSITE-ProRule:PRU00303}.
FT   CHAIN        25    397       Multidrug efflux pump subunit AcrA.
FT                                /FTId=PRO_0000043378.
FT   COILED       98    172       {ECO:0000250}.
FT   LIPID        25     25       N-palmitoyl cysteine.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00303}.
FT   LIPID        25     25       S-diacylglycerol cysteine.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00303}.
SQ   SEQUENCE   397 AA;  42197 MW;  5B81DD5BC2B0A077 CRC64;
     MNKNRGFTPL AVVLMLSGSL ALTGCDDKQA QQGGQQMPAV GVVTVKTEPL QITTELPGRT
     SAYRIAEVRP QVSGIILKRN FKEGSDIEAG VSLYQIDPAT YQATYDSAKG DLAKAQAAAN
     IAQLTVNRYQ KLLGTQYISK QEYDQALADA QQANAAVTAA KAAVETARIN LAYTKVTSPI
     SGRIGKSNVT EGALVQNGQA TALATVQQLD PIYVDVTQSS NDFLRLKQEL ANGTLKQENG
     KAKVSLITSD GIKFPQDGTL EFSDVTVDQT TGSITLRAIF PNPDHTLLPG MFVRARLEEG
     LNPNAILVPQ QGVTRTPRGD ATVLVVGADD KVETRPIVAS QAIGDKWLVT EGLKAGDRVV
     ISGLQKVRPG VQVKAQEVTA DNNQQAASGA QPEQSKS
//

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