(data stored in ACNUC7421 zone)

SWISSPROT: SELU_ECO57

ID   SELU_ECO57              Reviewed;         364 AA.
AC   Q8XCZ2; Q7AGV7;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   05-JUL-2017, entry version 91.
DE   RecName: Full=tRNA 2-selenouridine synthase {ECO:0000255|HAMAP-Rule:MF_01622};
DE            EC=2.9.1.- {ECO:0000255|HAMAP-Rule:MF_01622};
DE   AltName: Full=Selenophosphate-dependent tRNA 2-selenouridine synthase {ECO:0000255|HAMAP-Rule:MF_01622};
GN   Name=selU {ECO:0000255|HAMAP-Rule:MF_01622};
GN   OrderedLocusNames=Z0657, ECs0564;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the transfer of selenium from selenophosphate
CC       for conversion of 2-thiouridine to 2-selenouridine at the wobble
CC       position in tRNA. {ECO:0000255|HAMAP-Rule:MF_01622}.
CC   -!- CATALYTIC ACTIVITY: 5-methylaminomethyl-2-thiouridine +
CC       selenophosphate = 5-methylaminomethyl-2-selenouridine + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01622}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01622}.
CC   -!- SIMILARITY: Belongs to the 2-selenouridine synthase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01622}.
DR   EMBL; AE005174; AAG54859.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33987.1; -; Genomic_DNA.
DR   PIR; D90699; D90699.
DR   PIR; G85549; G85549.
DR   RefSeq; NP_308591.1; NC_002695.1.
DR   RefSeq; WP_001157987.1; NZ_MWVM01000032.1.
DR   ProteinModelPortal; Q8XCZ2; -.
DR   SMR; Q8XCZ2; -.
DR   STRING; 155864.Z0657; -.
DR   EnsemblBacteria; AAG54859; AAG54859; Z0657.
DR   EnsemblBacteria; BAB33987; BAB33987; BAB33987.
DR   GeneID; 915481; -.
DR   KEGG; ece:Z0657; -.
DR   KEGG; ecs:ECs0564; -.
DR   PATRIC; fig|386585.9.peg.672; -.
DR   eggNOG; ENOG4105CR7; Bacteria.
DR   eggNOG; COG2603; LUCA.
DR   HOGENOM; HOG000260000; -.
DR   KO; K06917; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0043828; F:tRNA 2-selenouridine synthase activity; IEA:InterPro.
DR   GO; GO:0070329; P:tRNA seleno-modification; IEA:InterPro.
DR   Gene3D; 3.40.250.10; -; 1.
DR   HAMAP; MF_01622; tRNA_sel_U_synth; 1.
DR   InterPro; IPR001763; Rhodanese-like_dom.
DR   InterPro; IPR017582; tRNA_2-selenouridine_synthase.
DR   SMART; SM00450; RHOD; 1.
DR   SUPFAM; SSF52821; SSF52821; 2.
DR   TIGRFAMs; TIGR03167; tRNA_sel_U_synt; 1.
DR   PROSITE; PS50206; RHODANESE_3; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8XCZ2.
DR   SWISS-2DPAGE; Q8XCZ2.
KW   Complete proteome; Selenium; Transferase.
FT   CHAIN         1    364       tRNA 2-selenouridine synthase.
FT                                /FTId=PRO_0000210860.
FT   DOMAIN       14    137       Rhodanese. {ECO:0000255|HAMAP-
FT                                Rule:MF_01622}.
FT   ACT_SITE     97     97       S-selanylcysteine intermediate.
FT                                {ECO:0000255|HAMAP-Rule:MF_01622}.
SQ   SEQUENCE   364 AA;  41074 MW;  5E1469B0EB409816 CRC64;
     MQERHTEQDY RALLIADTPI IDVRAPIEFE QGAMPAAINL PLMNNDERAA VGTCYKQQGS
     DAALALGHKL VAGEIRQQRM DAWRAACLQN PQGILCCARG GQRSHIVQSW LHAAGIDYPL
     VEGGYKALRQ TAIQATIELA QKPIVLIGGC TGSGKTLLVQ QQPNGVDLEG LARHRGSAFG
     RTLQPQLSQA SFENLLAAEM LKTDARQNLR LWVLEDESRM IGSNHLPECL RERMTQAAIA
     VVEDPFEIRL ERLNEEYFLR MHHDFTHAYG DEQGWQEYCE YLHHGLSAIK RRLGLQRYNE
     LAARLDAALT TQLTTGSTDG HLAWLVPLLE EYYDPMYRYQ LEKKAEKVVF RGEWAEVAEW
     VKAQ
//

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