(data stored in ACNUC7421 zone)

SWISSPROT: Q8XBU9_ECO57

ID   Q8XBU9_ECO57            Unreviewed;       137 AA.
AC   Q8XBU9; Q7AGR2;
DT   01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2002, sequence version 1.
DT   05-JUL-2017, entry version 105.
DE   RecName: Full=Proofreading thioesterase EntH {ECO:0000256|HAMAP-Rule:MF_00907};
DE            EC=3.1.2.- {ECO:0000256|HAMAP-Rule:MF_00907};
DE   AltName: Full=Enterobactin synthase component H {ECO:0000256|HAMAP-Rule:MF_00907};
GN   Name=ybdB {ECO:0000313|EMBL:AAG54932.1};
GN   Synonyms=entH {ECO:0000256|HAMAP-Rule:MF_00907};
GN   OrderedLocusNames=ECs0636 {ECO:0000313|EMBL:BAB34059.1}, Z0739
GN   {ECO:0000313|EMBL:AAG54932.1};
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334 {ECO:0000313|Proteomes:UP000002519};
RN   [1] {ECO:0000313|EMBL:BAB34059.1, ECO:0000313|Proteomes:UP000000558}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC
RC   {ECO:0000313|Proteomes:UP000000558}, and Sakai
RC   {ECO:0000313|EMBL:BAB34059.1};
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [2] {ECO:0000313|EMBL:AAG54932.1, ECO:0000313|Proteomes:UP000002519}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EDL933 {ECO:0000313|EMBL:AAG54932.1}, and O157:H7 / EDL933 /
RC   ATCC 700927 / EHEC {ECO:0000313|Proteomes:UP000002519};
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G.III., Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
CC   -!- FUNCTION: Required for optimal enterobactin synthesis. Acts as a
CC       proofreading enzyme that prevents EntB misacylation by hydrolyzing
CC       the thioester bound existing between EntB and wrongly charged
CC       molecules. {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- SUBUNIT: Homotetramer. Dimer of dimers. Interacts specifically
CC       with the aryl carrier protein (ArCP) domain of EntB.
CC       {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00907}.
CC   -!- SIMILARITY: Belongs to the thioesterase PaaI family.
CC       {ECO:0000256|HAMAP-Rule:MF_00907}.
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DR   EMBL; AE005174; AAG54932.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34059.1; -; Genomic_DNA.
DR   PIR; D90708; D90708.
DR   PIR; H85558; H85558.
DR   RefSeq; NP_308663.1; NC_002695.1.
DR   RefSeq; WP_000637937.1; NZ_MWVM01000011.1.
DR   SMR; Q8XBU9; -.
DR   STRING; 155864.Z0739; -.
DR   EnsemblBacteria; AAG54932; AAG54932; Z0739.
DR   EnsemblBacteria; BAB34059; BAB34059; BAB34059.
DR   GeneID; 916995; -.
DR   KEGG; ece:Z0739; -.
DR   KEGG; ecs:ECs0636; -.
DR   PATRIC; fig|386585.9.peg.746; -.
DR   eggNOG; ENOG4108TZA; Bacteria.
DR   eggNOG; COG2050; LUCA.
DR   HOGENOM; HOG000066991; -.
DR   UniPathway; UPA00017; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016790; F:thiolester hydrolase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009239; P:enterobactin biosynthetic process; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00907; Thioesterase_EntH; 1.
DR   InterPro; IPR029069; HotDog_dom.
DR   InterPro; IPR003736; PAAI_dom.
DR   InterPro; IPR026576; Thioesterase_EntH.
DR   InterPro; IPR006683; Thioestr_dom.
DR   Pfam; PF03061; 4HBT; 1.
DR   SUPFAM; SSF54637; SSF54637; 1.
DR   TIGRFAMs; TIGR00369; unchar_dom_1; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8XBU9.
DR   SWISS-2DPAGE; Q8XBU9.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000558,
KW   ECO:0000313|Proteomes:UP000002519};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00907};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00907}.
FT   DOMAIN       50    127       4HBT. {ECO:0000259|Pfam:PF03061}.
FT   ACT_SITE     63     63       Nucleophile or proton acceptor.
FT                                {ECO:0000256|HAMAP-Rule:MF_00907}.
SQ   SEQUENCE   137 AA;  14982 MW;  66D16D18C8093F1A CRC64;
     MIWKRHLTLD ELNATSDNTM VAHLGIVYTR LGDDVLEAEM PVDIRTHQPF GLLHGGASAA
     LAETLGSMAG FMMTRDGQCV VGTELNATHH RPVSEGKVRG VCQPLHLGRQ NQSWEIVVFD
     EQGRRCCTCR LGTAVLG
//

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