(data stored in ACNUC7421 zone)

SWISSPROT: A0A0H3JGB8_ECO57

ID   A0A0H3JGB8_ECO57        Unreviewed;       510 AA.
AC   A0A0H3JGB8;
DT   16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT   16-SEP-2015, sequence version 1.
DT   08-MAY-2019, entry version 20.
DE   RecName: Full=Citrate lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE            Short=Citrase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE            EC=2.8.3.10 {ECO:0000256|PIRNR:PIRNR009451};
DE            EC=4.1.3.6 {ECO:0000256|PIRNR:PIRNR009451};
DE   AltName: Full=Citrate (pro-3S)-lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE   AltName: Full=Citrate CoA-transferase subunit {ECO:0000256|PIRNR:PIRNR009451};
GN   Name=citF {ECO:0000313|EMBL:BAB34077.1};
GN   ORFNames=ECs_0654 {ECO:0000313|EMBL:BAB34077.1};
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334 {ECO:0000313|EMBL:BAB34077.1, ECO:0000313|Proteomes:UP000000558};
RN   [1] {ECO:0000313|EMBL:BAB34077.1, ECO:0000313|Proteomes:UP000000558}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 /
RC   EHEC {ECO:0000313|Proteomes:UP000000558};
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + citrate = (3S)-citryl-CoA + acetate;
CC         Xref=Rhea:RHEA:19405, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57321; EC=2.8.3.10;
CC         Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=citrate = acetate + oxaloacetate; Xref=Rhea:RHEA:10760,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089;
CC         EC=4.1.3.6; Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR009451}.
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DR   EMBL; BA000007; BAB34077.1; -; Genomic_DNA.
DR   RefSeq; NP_308681.1; NC_002695.1.
DR   STRING; 155864.EDL933_0688; -.
DR   EnsemblBacteria; BAB34077; BAB34077; BAB34077.
DR   GeneID; 917014; -.
DR   KEGG; ecs:ECs0654; -.
DR   PATRIC; fig|386585.9.peg.765; -.
DR   KO; K01643; -.
DR   Proteomes; UP000000558; Chromosome.
DR   GO; GO:0009346; C:citrate lyase complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0008815; F:citrate (pro-3S)-lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008814; F:citrate CoA-transferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:UniProtKB-UniRule.
DR   InterPro; IPR006472; Citrate_lyase_asu.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR40596; PTHR40596; 1.
DR   Pfam; PF04223; CitF; 1.
DR   PIRSF; PIRSF009451; Citrt_lyas_alpha; 1.
DR   SUPFAM; SSF100950; SSF100950; 2.
DR   TIGRFAMs; TIGR01584; citF; 1.
PE   4: Predicted;
DR   PRODOM; A0A0H3JGB8.
DR   SWISS-2DPAGE; A0A0H3JGB8.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000558};
KW   Cytoplasm {ECO:0000256|PIRNR:PIRNR009451};
KW   Lyase {ECO:0000256|PIRNR:PIRNR009451, ECO:0000313|EMBL:BAB34077.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000558};
KW   Transferase {ECO:0000256|PIRNR:PIRNR009451}.
SQ   SEQUENCE   510 AA;  55117 MW;  42ABFF6EEA5C87A0 CRC64;
     MTQKIEQSQR QERVAAWNRR AECDLAAFQN SPKQTYQAEK ALDRKLCANL EEAIRRSGLQ
     DGMTVSFHHA FRGGDLTVNM VMDVIAKMGF KNLILASSSL SDCHAPLVEH IRQGVVTRIY
     TSGLRGPLAE EISRGLLAEP VQIHSHGGRV HLVQSGELNI DVAFLGVPSC DEFGNANGYT
     GKACCGSLGY AMVDADNAKQ VVMLTEELLP YPHNPASIEQ DQVDLIVKVD RVGDAAKIGA
     GATRMTTNPR ELLIARSAAD VIVNSGYFKE GFSMQTGTGG ASLAVTLFLE DKMRSRDIRA
     DFALGGITAT MVDLHEKGLI RKLLDVQSFD SHAAQSLARN PNHIEISANQ YANWGSKGAS
     VDRLDVVVLS ALEIDTQFNV NVLTGSDGVL RGASGGHCDT AIASALSIIV APLVRGRIPT
     LVDNVLTCIT PGSSVDILVT DHGIAVNPAR PELAERLQEA GIKVVSIEWL RERARLLTGE
     PQPIEFTDRV VAVVRYRDGS VIDVVHQVKE
//

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