(data stored in ACNUC7421 zone)

SWISSPROT: RODA_ECO57

ID   RODA_ECO57              Reviewed;         370 AA.
AC   P0ABG8; P13409; P15035;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   08-MAY-2019, entry version 85.
DE   RecName: Full=Peptidoglycan glycosyltransferase MrdB {ECO:0000255|HAMAP-Rule:MF_02079};
DE            Short=PGT {ECO:0000255|HAMAP-Rule:MF_02079};
DE            EC=2.4.1.129 {ECO:0000255|HAMAP-Rule:MF_02079};
DE   AltName: Full=Cell elongation protein RodA {ECO:0000255|HAMAP-Rule:MF_02079};
DE   AltName: Full=Cell wall polymerase {ECO:0000255|HAMAP-Rule:MF_02079};
DE   AltName: Full=Peptidoglycan polymerase {ECO:0000255|HAMAP-Rule:MF_02079};
DE            Short=PG polymerase {ECO:0000255|HAMAP-Rule:MF_02079};
GN   Name=mrdB {ECO:0000255|HAMAP-Rule:MF_02079}; Synonyms=rodA;
GN   OrderedLocusNames=Z0780, ECs0672;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Peptidoglycan polymerase that is essential for cell wall
CC       elongation. {ECO:0000255|HAMAP-Rule:MF_02079}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-
CC         D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + beta-D-
CC         GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-
CC         diphospho-di-trans,octa-cis-undecaprenol = [GlcNAc-(1->4)-
CC         Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-
CC         di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl
CC         diphosphate + H(+); Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602,
CC         Rhea:RHEA-COMP:9603, ChEBI:CHEBI:15378, ChEBI:CHEBI:58405,
CC         ChEBI:CHEBI:60033, ChEBI:CHEBI:78435; EC=2.4.1.129;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_02079};
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_02079}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_02079}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_02079}.
CC   -!- SIMILARITY: Belongs to the SEDS family. MrdB/RodA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02079}.
DR   EMBL; AE005174; AAG54968.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34095.1; -; Genomic_DNA.
DR   PIR; D85563; D85563.
DR   PIR; H90712; H90712.
DR   RefSeq; NP_308699.1; NC_002695.1.
DR   RefSeq; WP_000131719.1; NZ_SDVX01000001.1.
DR   STRING; 155864.EDL933_0708; -.
DR   PRIDE; P0ABG8; -.
DR   EnsemblBacteria; AAG54968; AAG54968; Z0780.
DR   EnsemblBacteria; BAB34095; BAB34095; BAB34095.
DR   GeneID; 917033; -.
DR   KEGG; ece:Z0780; -.
DR   KEGG; ecs:ECs0672; -.
DR   PATRIC; fig|386585.9.peg.784; -.
DR   eggNOG; ENOG4105CNI; Bacteria.
DR   eggNOG; COG0772; LUCA.
DR   HOGENOM; HOG000282686; -.
DR   KO; K05837; -.
DR   BioCyc; ECOO157:MRDB-MONOMER; -.
DR   UniPathway; UPA00219; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0051301; P:cell division; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   HAMAP; MF_02079; PGT_RodA; 1.
DR   InterPro; IPR018365; Cell_cycle_FtsW-rel_CS.
DR   InterPro; IPR001182; FtsW/RodA.
DR   InterPro; IPR011923; RodA/MrdB.
DR   PANTHER; PTHR30474; PTHR30474; 1.
DR   PANTHER; PTHR30474:SF1; PTHR30474:SF1; 1.
DR   Pfam; PF01098; FTSW_RODA_SPOVE; 1.
DR   TIGRFAMs; TIGR02210; rodA_shape; 1.
DR   PROSITE; PS00428; FTSW_RODA_SPOVE; 1.
PE   3: Inferred from homology;
DR   PRODOM; P0ABG8.
DR   SWISS-2DPAGE; P0ABG8.
KW   Cell inner membrane; Cell membrane; Cell shape;
KW   Cell wall biogenesis/degradation; Complete proteome;
KW   Glycosyltransferase; Membrane; Peptidoglycan synthesis;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN         1    370       Peptidoglycan glycosyltransferase MrdB.
FT                                /FTId=PRO_0000062716.
FT   TRANSMEM     20     40       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM     50     70       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM     75     95       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM    136    156       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM    160    180       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM    183    203       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM    263    283       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM    312    332       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
FT   TRANSMEM    336    356       Helical. {ECO:0000255|HAMAP-
FT                                Rule:MF_02079}.
SQ   SEQUENCE   370 AA;  40476 MW;  68FCDF8D6B123D69 CRC64;
     MTDNPNKKTF WDKVHLDPTM LLILLALLVY SALVIWSASG QDIGMMERKI GQIAMGLVIM
     VVMAQIPPRV YEGWAPYLYI ICIILLVAVD AFGAISKGAQ RWLDLGIVRF QPSEIAKIAV
     PLMVARFINR DVCPPSLKNT GIALVLIFMP TLLVAAQPDL GTSILVALSG LFVLFLSGLS
     WRLIGVAVVL VAAFIPILWF FLMHDYQRQR VMMLLDPESD PLGAGYHIIQ SKIAIGSGGL
     RGKGWLHGTQ SQLEFLPERH TDFIFAVLAE ELGLVGILIL LALYILLIMR GLWIAARAQT
     TFGRVMAGGL MLILFVYVFV NIGMVSGILP VVGVPLPLVS YGGSALIVLM AGFGIVMSIH
     THRKMLSKSV
//

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