(data stored in ACNUC7421 zone)

SWISSPROT: RLMH_ECO57

ID   RLMH_ECO57              Reviewed;         155 AA.
AC   P0A8J0; P05850;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   05-JUL-2017, entry version 85.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase H {ECO:0000255|HAMAP-Rule:MF_00658};
DE            EC=2.1.1.177 {ECO:0000255|HAMAP-Rule:MF_00658};
DE   AltName: Full=23S rRNA (pseudouridine1915-N3)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00658};
DE   AltName: Full=23S rRNA m3Psi1915 methyltransferase {ECO:0000255|HAMAP-Rule:MF_00658};
DE   AltName: Full=rRNA (pseudouridine-N3-)-methyltransferase RlmH {ECO:0000255|HAMAP-Rule:MF_00658};
GN   Name=rlmH {ECO:0000255|HAMAP-Rule:MF_00658};
GN   OrderedLocusNames=Z0782, ECs0674;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Specifically methylates the pseudouridine at position
CC       1915 (m3Psi1915) in 23S rRNA. {ECO:0000255|HAMAP-Rule:MF_00658}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + pseudouridine(1915)
CC       in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-
CC       methylpseudouridine(1915) in 23S rRNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00658}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00658}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00658}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RlmH family.
CC       {ECO:0000255|HAMAP-Rule:MF_00658}.
DR   EMBL; AE005174; AAG54970.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34097.1; -; Genomic_DNA.
DR   PIR; B90713; B90713.
DR   PIR; F85563; F85563.
DR   RefSeq; NP_308701.1; NC_002695.1.
DR   RefSeq; WP_000776104.1; NZ_MWVM01000011.1.
DR   ProteinModelPortal; P0A8J0; -.
DR   SMR; P0A8J0; -.
DR   MINT; MINT-1251135; -.
DR   STRING; 155864.Z0782; -.
DR   EnsemblBacteria; AAG54970; AAG54970; Z0782.
DR   EnsemblBacteria; BAB34097; BAB34097; BAB34097.
DR   GeneID; 917035; -.
DR   KEGG; ece:Z0782; -.
DR   KEGG; ecs:ECs0674; -.
DR   PATRIC; fig|386585.9.peg.786; -.
DR   eggNOG; ENOG4108UXA; Bacteria.
DR   eggNOG; COG1576; LUCA.
DR   HOGENOM; HOG000218434; -.
DR   KO; K00783; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_00658; 23SrRNA_methyltr_H; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR003742; RlmH-like.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR33603:SF2; PTHR33603:SF2; 1.
DR   Pfam; PF02590; SPOUT_MTase; 1.
DR   PIRSF; PIRSF004505; MT_bac; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00246; tRNA_RlmH_YbeA; 1.
PE   3: Inferred from homology;
DR   PRODOM; P0A8J0.
DR   SWISS-2DPAGE; P0A8J0.
KW   Complete proteome; Cytoplasm; Methyltransferase; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    155       Ribosomal RNA large subunit
FT                                methyltransferase H.
FT                                /FTId=PRO_0000198116.
SQ   SEQUENCE   155 AA;  17341 MW;  DFA0EFCF4EB057E7 CRC64;
     MKLQLVAVGT KMPDWVQTGF TEYLRRFPKD MPFELIEIPA GKRGKNADIK RILDKEGEQM
     LAAAGKNRIV TLDIPGKPWD TPQLAAELER WKLDGRDVSL LIGGPEGLSP ACKAAAEQSW
     SLSALTLPHP LVRVLVAESL YRAWSITTNH PYHRE
//

If you have problems or comments...

PBIL Back to PBIL home page