(data stored in ACNUC7421 zone)

SWISSPROT: Q8X9G7_ECO57

ID   Q8X9G7_ECO57            Unreviewed;       181 AA.
AC   Q8X9G7; Q7AGM3;
DT   01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2002, sequence version 1.
DT   05-JUL-2017, entry version 114.
DE   RecName: Full=Negative modulator of initiation of replication {ECO:0000256|HAMAP-Rule:MF_00908, ECO:0000256|PIRNR:PIRNR019401};
GN   Name=seqA {ECO:0000256|HAMAP-Rule:MF_00908,
GN   ECO:0000313|EMBL:AAG55010.1};
GN   OrderedLocusNames=ECs0718 {ECO:0000313|EMBL:BAB34141.1}, Z0836
GN   {ECO:0000313|EMBL:AAG55010.1};
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334 {ECO:0000313|Proteomes:UP000002519};
RN   [1] {ECO:0000313|EMBL:BAB34141.1, ECO:0000313|Proteomes:UP000000558}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC
RC   {ECO:0000313|Proteomes:UP000000558}, and Sakai
RC   {ECO:0000313|EMBL:BAB34141.1};
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [2] {ECO:0000313|EMBL:AAG55010.1, ECO:0000313|Proteomes:UP000002519}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EDL933 {ECO:0000313|EMBL:AAG55010.1}, and O157:H7 / EDL933 /
RC   ATCC 700927 / EHEC {ECO:0000313|Proteomes:UP000002519};
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G.III., Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
CC   -!- FUNCTION: Negative regulator of replication initiation, which
CC       contributes to regulation of DNA replication and ensures that
CC       replication initiation occurs exactly once per chromosome per cell
CC       cycle. Binds to pairs of hemimethylated GATC sequences in the oriC
CC       region, thus preventing assembly of replication proteins and re-
CC       initiation at newly replicated origins. Repression is relieved
CC       when the region becomes fully methylated. {ECO:0000256|HAMAP-
CC       Rule:MF_00908, ECO:0000256|PIRNR:PIRNR019401}.
CC   -!- SUBUNIT: Homodimer. Polymerizes to form helical filaments.
CC       {ECO:0000256|HAMAP-Rule:MF_00908}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00908,
CC       ECO:0000256|PIRNR:PIRNR019401}.
CC   -!- SIMILARITY: Belongs to the SeqA family. {ECO:0000256|HAMAP-
CC       Rule:MF_00908, ECO:0000256|PIRNR:PIRNR019401}.
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DR   EMBL; AE005174; AAG55010.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34141.1; -; Genomic_DNA.
DR   PIR; F85568; F85568.
DR   PIR; F90718; F90718.
DR   RefSeq; NP_308745.1; NC_002695.1.
DR   RefSeq; WP_001302000.1; NZ_MWVM01000011.1.
DR   ProteinModelPortal; Q8X9G7; -.
DR   STRING; 155864.Z0836; -.
DR   EnsemblBacteria; AAG55010; AAG55010; Z0836.
DR   EnsemblBacteria; BAB34141; BAB34141; BAB34141.
DR   GeneID; 917087; -.
DR   KEGG; ece:Z0836; -.
DR   KEGG; ecs:ECs0718; -.
DR   PATRIC; fig|386585.9.peg.834; -.
DR   eggNOG; ENOG4107CDU; Bacteria.
DR   eggNOG; COG3057; LUCA.
DR   KO; K03645; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0032297; P:negative regulation of DNA-dependent DNA replication initiation; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 1.20.1380.10; -; 1.
DR   HAMAP; MF_00908; SeqA; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR005621; SeqA.
DR   InterPro; IPR026577; SeqA_DNA-bd_C.
DR   InterPro; IPR033761; SeqA_N.
DR   Pfam; PF03925; SeqA; 1.
DR   Pfam; PF17206; SeqA_N; 1.
DR   PIRSF; PIRSF019401; SeqA; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF82808; SSF82808; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q8X9G7.
DR   SWISS-2DPAGE; Q8X9G7.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000558,
KW   ECO:0000313|Proteomes:UP000002519};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00908,
KW   ECO:0000256|PIRNR:PIRNR019401};
KW   DNA replication inhibitor {ECO:0000256|HAMAP-Rule:MF_00908,
KW   ECO:0000256|PIRNR:PIRNR019401};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00908,
KW   ECO:0000256|PIRNR:PIRNR019401}.
FT   DOMAIN        1     35       SeqA_N. {ECO:0000259|Pfam:PF17206}.
FT   DOMAIN       71    179       SeqA. {ECO:0000259|Pfam:PF03925}.
FT   REGION       87     88       Interaction with DNA. {ECO:0000256|HAMAP-
FT                                Rule:MF_00908}.
FT   REGION      116    120       Interaction with DNA. {ECO:0000256|HAMAP-
FT                                Rule:MF_00908}.
FT   REGION      150    156       Interaction with DNA. {ECO:0000256|HAMAP-
FT                                Rule:MF_00908}.
SQ   SEQUENCE   181 AA;  20329 MW;  27C6078CA3C82293 CRC64;
     MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR VASPAIVEAK
     PIKTIKDKVR AMRELLLSDE YAEQKRAVNR FMLLLSTLYS LDAQAFAEAT ESLHGRTRVY
     FAADEQTLLK NGNQTKPKHV PGTPYWVITN TNTGRKCSMI EHIMQSMQFP AELIEKVCGT
     I
//

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