(data stored in ACNUC7421 zone)

SWISSPROT: Q82QG1_STRAW

ID   Q82QG1_STRAW            Unreviewed;       225 AA.
AC   Q82QG1;
DT   01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2003, sequence version 1.
DT   08-MAY-2019, entry version 107.
DE   RecName: Full=GTP cyclohydrolase-2 {ECO:0000256|HAMAP-Rule:MF_00179};
DE            EC=3.5.4.25 {ECO:0000256|HAMAP-Rule:MF_00179};
DE   AltName: Full=GTP cyclohydrolase II {ECO:0000256|HAMAP-Rule:MF_00179};
GN   Name=ribA4 {ECO:0000313|EMBL:BAC68255.1};
GN   Synonyms=ribA {ECO:0000256|HAMAP-Rule:MF_00179};
GN   ORFNames=SAVERM_545 {ECO:0000313|EMBL:BAC68255.1};
OS   Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 /
OS   NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=227882 {ECO:0000313|EMBL:BAC68255.1, ECO:0000313|Proteomes:UP000000428};
RN   [1] {ECO:0000313|EMBL:BAC68255.1, ECO:0000313|Proteomes:UP000000428}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 /
RC   NRRL 8165 / MA-4680 {ECO:0000313|Proteomes:UP000000428};
RX   PubMed=11572948; DOI=10.1073/pnas.211433198;
RA   Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C.,
RA   Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T.,
RA   Kikuchi H., Shiba T., Sakaki Y., Hattori M.;
RT   "Genome sequence of an industrial microorganism Streptomyces
RT   avermitilis: deducing the ability of producing secondary
RT   metabolites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
RN   [2] {ECO:0000313|EMBL:BAC68255.1, ECO:0000313|Proteomes:UP000000428}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 /
RC   NRRL 8165 / MA-4680 {ECO:0000313|Proteomes:UP000000428};
RX   PubMed=12692562; DOI=10.1038/nbt820;
RA   Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M., Omura S.;
RT   "Complete genome sequence and comparative analysis of the industrial
RT   microorganism Streptomyces avermitilis.";
RL   Nat. Biotechnol. 21:526-531(2003).
CC   -!- FUNCTION: Catalyzes the conversion of GTP to 2,5-diamino-6-
CC       ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and
CC       pyrophosphate. {ECO:0000256|HAMAP-Rule:MF_00179}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + 3 H2O = 2,5-diamino-6-hydroxy-4-(5-
CC         phosphoribosylamino)-pyrimidine + diphosphate + formate + 2
CC         H(+); Xref=Rhea:RHEA:23704, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:37565, ChEBI:CHEBI:58614; EC=3.5.4.25;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00179,
CC         ECO:0000256|SAAS:SAAS01116087};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00179};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00179};
CC   -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-
CC       6-(D-ribitylamino)uracil from GTP: step 1/4. {ECO:0000256|HAMAP-
CC       Rule:MF_00179}.
CC   -!- SIMILARITY: Belongs to the GTP cyclohydrolase II family.
CC       {ECO:0000256|HAMAP-Rule:MF_00179}.
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DR   EMBL; BA000030; BAC68255.1; -; Genomic_DNA.
DR   RefSeq; WP_010981984.1; NZ_JZJK01000088.1.
DR   STRING; 227882.SAV_545; -.
DR   EnsemblBacteria; BAC68255; BAC68255; SAVERM_545.
DR   KEGG; sma:SAVERM_545; -.
DR   eggNOG; ENOG4108S0I; Bacteria.
DR   eggNOG; COG0807; LUCA.
DR   HOGENOM; HOG000115442; -.
DR   KO; K01497; -.
DR   OMA; EMGLHEV; -.
DR   OrthoDB; 900513at2; -.
DR   BioCyc; SAVE227882:G1G23-616-MONOMER; -.
DR   UniPathway; UPA00275; UER00400.
DR   Proteomes; UP000000428; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003935; F:GTP cyclohydrolase II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009231; P:riboflavin biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00641; GTP_cyclohydro2; 1.
DR   Gene3D; 3.40.50.10990; -; 1.
DR   HAMAP; MF_00179; RibA; 1.
DR   InterPro; IPR032677; GTP_cyclohydro_II.
DR   InterPro; IPR000926; RibA.
DR   InterPro; IPR036144; RibA-like_sf.
DR   Pfam; PF00925; GTP_cyclohydro2; 1.
DR   SUPFAM; SSF142695; SSF142695; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q82QG1.
DR   SWISS-2DPAGE; Q82QG1.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000428};
KW   GTP-binding {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711691};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS01033620, ECO:0000313|EMBL:BAC68255.1};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711702};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711707};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000428};
KW   Riboflavin biosynthesis {ECO:0000256|HAMAP-Rule:MF_00179,
KW   ECO:0000256|SAAS:SAAS00711711};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   DOMAIN       28    193       GTP_cyclohydro2. {ECO:0000259|Pfam:
FT                                PF00925}.
FT   NP_BIND      73     77       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   NP_BIND     115    117       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   ACT_SITE    149    149       Proton acceptor. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   ACT_SITE    151    151       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   METAL        78     78       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   METAL        89     89       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   METAL        91     91       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00179}.
FT   BINDING      94     94       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   BINDING     137    137       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   BINDING     172    172       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
FT   BINDING     177    177       GTP. {ECO:0000256|HAMAP-Rule:MF_00179}.
SQ   SEQUENCE   225 AA;  24292 MW;  CD5147D142F6424D CRC64;
     MRDSRNATAS SDSRNPTGLS ATTVRTRVTV PLRFGDGCTV TADLATFHHL ADGQEHVAVI
     LGEPAAGRPP LVRLHSECLT GDVFGSARCD CGPQLREALE RIADRGGVLL YLRQEGRGIG
     LYNKLDAYAL QDQGLDTYEA NTTLGLPEDA RDYTAAAQML AALGIFELDL LSNNPDKAAQ
     LRTLGLTVTQ HVPTGVFTTA HNIRYLRTKV VRTQHTLPLT ELTVG
//

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