(data stored in ACNUC7421 zone)

SWISSPROT: HOA_STRGG

ID   HOA_STRGG               Reviewed;         340 AA.
AC   B1VRH5;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   30-AUG-2017, entry version 50.
DE   RecName: Full=4-hydroxy-2-oxovalerate aldolase {ECO:0000255|HAMAP-Rule:MF_01656};
DE            Short=HOA {ECO:0000255|HAMAP-Rule:MF_01656};
DE            EC=4.1.3.39 {ECO:0000255|HAMAP-Rule:MF_01656};
DE   AltName: Full=4-hydroxy-2-keto-pentanoic acid aldolase {ECO:0000255|HAMAP-Rule:MF_01656};
DE   AltName: Full=4-hydroxy-2-oxopentanoate aldolase {ECO:0000255|HAMAP-Rule:MF_01656};
GN   OrderedLocusNames=SGR_564;
OS   Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=455632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 4626 / NBRC 13350;
RX   PubMed=18375553; DOI=10.1128/JB.00204-08;
RA   Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H.,
RA   Yamashita A., Hattori M., Horinouchi S.;
RT   "Genome sequence of the streptomycin-producing microorganism
RT   Streptomyces griseus IFO 13350.";
RL   J. Bacteriol. 190:4050-4060(2008).
CC   -!- FUNCTION: Catalyzes the retro-aldol cleavage of 4-hydroxy-2-
CC       oxopentanoate to pyruvate and acetaldehyde. Is involved in the
CC       meta-cleavage pathway for the degradation of aromatic compounds.
CC       {ECO:0000255|HAMAP-Rule:MF_01656}.
CC   -!- CATALYTIC ACTIVITY: (S)-4-hydroxy-2-oxopentanoate = acetaldehyde +
CC       pyruvate. {ECO:0000255|HAMAP-Rule:MF_01656}.
CC   -!- SIMILARITY: Belongs to the 4-hydroxy-2-oxovalerate aldolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01656}.
DR   EMBL; AP009493; BAG17393.1; -; Genomic_DNA.
DR   RefSeq; WP_003964429.1; NC_010572.1.
DR   ProteinModelPortal; B1VRH5; -.
DR   SMR; B1VRH5; -.
DR   STRING; 455632.SGR_564; -.
DR   EnsemblBacteria; BAG17393; BAG17393; SGR_564.
DR   GeneID; 6212303; -.
DR   KEGG; sgr:SGR_564; -.
DR   eggNOG; ENOG4105DF3; Bacteria.
DR   eggNOG; COG0119; LUCA.
DR   HOGENOM; HOG000048047; -.
DR   KO; K01666; -.
DR   OMA; IRVACYI; -.
DR   Proteomes; UP000001685; Chromosome.
DR   GO; GO:0008701; F:4-hydroxy-2-oxovalerate aldolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd07943; DRE_TIM_HOA; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_01656; HOA; 1.
DR   InterPro; IPR017629; 4OH_2_O-val_aldolase.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR012425; DmpG_comm.
DR   InterPro; IPR035685; DRE_TIM_HOA.
DR   InterPro; IPR000891; PYR_CT.
DR   Pfam; PF07836; DmpG_comm; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   ProDom; PD005364; DmpG_comm; 1.
DR   TIGRFAMs; TIGR03217; 4OH_2_O_val_ald; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
DR   PRODOM; B1VRH5.
DR   SWISS-2DPAGE; B1VRH5.
KW   Aromatic hydrocarbons catabolism; Complete proteome; Lyase; Manganese;
KW   Metal-binding; Reference proteome.
FT   CHAIN         1    340       4-hydroxy-2-oxovalerate aldolase.
FT                                /FTId=PRO_0000387925.
FT   DOMAIN        4    255       Pyruvate carboxyltransferase.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01151}.
FT   REGION       12     13       Substrate binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01656}.
FT   ACT_SITE     16     16       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_01656}.
FT   METAL        13     13       Manganese. {ECO:0000255|HAMAP-
FT                                Rule:MF_01656}.
FT   METAL       194    194       Manganese; via tele nitrogen.
FT                                {ECO:0000255|HAMAP-Rule:MF_01656}.
FT   METAL       196    196       Manganese; via tele nitrogen.
FT                                {ECO:0000255|HAMAP-Rule:MF_01656}.
FT   BINDING     166    166       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01656}.
FT   BINDING     194    194       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01656}.
FT   SITE         12     12       Transition state stabilizer.
FT                                {ECO:0000255|HAMAP-Rule:MF_01656}.
SQ   SEQUENCE   340 AA;  35230 MW;  F4B77A0872B443F3 CRC64;
     MKQVVIHDPT LRDGQHAVHH RLGLTELRRY AEAADAARVP VVEVGHGNGL GASSLQVGLA
     AATDDAMLST VREALRHSRL GTFMLPGWGT SDDLRRAISH GVDVFRVGVH ATEASLAEHH
     LGFLRDAGAE AHCVLMMSHM ASPGELAEQA ARAVGYGAQA VGIMDSAGHF LPPDVTARIG
     AIVEAVGTVP VIFHGHNNLG MAVANSVAAA EAGARIIDGC ARGFGAGAGN TQLEVLVPVL
     ERSGFATGID LYALLDAADL AERELMPAPP VPASMSIVSG LAGVFSGFKH RVVELAGAAG
     VDPREVFFEL GRRQAIAGQE DLIVDVVAEL SGRGPKTDAA
//

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