(data stored in ACNUC10821 zone)

SWISSPROT: C8U2D3_ECO10

ID   C8U2D3_ECO10            Unreviewed;       272 AA.
AC   C8U2D3;
DT   03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT   03-NOV-2009, sequence version 1.
DT   08-MAY-2019, entry version 70.
DE   RecName: Full=HMP-PP phosphatase {ECO:0000256|HAMAP-Rule:MF_01847, ECO:0000256|SAAS:SAAS00662854};
DE            EC=3.6.1.- {ECO:0000256|HAMAP-Rule:MF_01847};
GN   Name=cof {ECO:0000256|HAMAP-Rule:MF_01847,
GN   ECO:0000313|EMBL:BAI29291.1};
GN   OrderedLocusNames=ECO103_0423 {ECO:0000313|EMBL:BAI29291.1};
OS   Escherichia coli O103:H2 (strain 12009 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585395 {ECO:0000313|EMBL:BAI29291.1, ECO:0000313|Proteomes:UP000000959};
RN   [1] {ECO:0000313|EMBL:BAI29291.1, ECO:0000313|Proteomes:UP000000959}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12009 / EHEC {ECO:0000313|Proteomes:UP000000959};
RX   PubMed=19815525; DOI=10.1073/pnas.0903585106;
RA   Ogura Y., Ooka T., Iguchi A., Toh H., Asadulghani M., Oshima K.,
RA   Kodama T., Abe H., Nakayama K., Kurokawa K., Tobe T., Hattori M.,
RA   Hayashi T.;
RT   "Comparative genomics reveal the mechanism of the parallel evolution
RT   of O157 and non-O157 enterohemorrhagic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:17939-17944(2009).
CC   -!- FUNCTION: Catalyzes the hydrolysis of 4-amino-2-methyl-5-
CC       hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2-
CC       methyl-5-hydroxymethylpyrimidine phosphate (HMP-P).
CC       {ECO:0000256|HAMAP-Rule:MF_01847, ECO:0000256|SAAS:SAAS00693533}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H2O =
CC         4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + H(+) +
CC         phosphate; Xref=Rhea:RHEA:27914, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57841,
CC         ChEBI:CHEBI:58354; Evidence={ECO:0000256|HAMAP-Rule:MF_01847};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01847, ECO:0000256|SAAS:SAAS00693531};
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. Cof
CC       family. {ECO:0000256|HAMAP-Rule:MF_01847,
CC       ECO:0000256|SAAS:SAAS00693530}.
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DR   EMBL; AP010958; BAI29291.1; -; Genomic_DNA.
DR   RefSeq; WP_000113027.1; NC_013353.1.
DR   SMR; C8U2D3; -.
DR   EnsemblBacteria; BAI29291; BAI29291; ECO103_0423.
DR   KEGG; eoh:ECO103_0423; -.
DR   HOGENOM; HOG000184784; -.
DR   KO; K11938; -.
DR   OMA; MPDHRLG; -.
DR   BioCyc; ECOL585395:ECO103_RS02215-MONOMER; -.
DR   Proteomes; UP000000959; Chromosome.
DR   GO; GO:0016818; F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016791; F:phosphatase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01847; HMP_PP_phosphat; 1.
DR   InterPro; IPR000150; Cof.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006379; HAD-SF_hydro_IIB.
DR   InterPro; IPR023938; HMP-PP_phosphatase.
DR   PANTHER; PTHR47267:SF2; PTHR47267:SF2; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR00099; Cof-subfamily; 1.
DR   TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
PE   3: Inferred from homology;
DR   PRODOM; C8U2D3.
DR   SWISS-2DPAGE; C8U2D3.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000959};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01847,
KW   ECO:0000256|SAAS:SAAS00693535, ECO:0000313|EMBL:BAI29291.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01847,
KW   ECO:0000256|SAAS:SAAS00693534};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_01847,
KW   ECO:0000256|SAAS:SAAS00693536}.
FT   ACT_SITE      8      8       Nucleophile. {ECO:0000256|HAMAP-Rule:
FT                                MF_01847}.
FT   METAL         8      8       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_01847}.
FT   METAL        10     10       Magnesium; via carbonyl oxygen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01847}.
FT   METAL       212    212       Magnesium. {ECO:0000256|HAMAP-Rule:
FT                                MF_01847}.
SQ   SEQUENCE   272 AA;  30329 MW;  D8FC03565373EADC CRC64;
     MARLAAFDMD GTLLMPDHHL GEKTLSTLAR LRERDITLTF ATGRHALEMQ HILGALSLDA
     YLITGNGTRV HSLEGELLHR DDLPADVAEL VLYQQWDTRA SMHIFNDDGW FTGKEIPALL
     QAFVYSGFRY QIIDVKKMPL GSVTKICFCG DHDDLTRLQI QLYEALGERA HLCFSATDCL
     EVLPVGCNKG AALTVLTQHL GLSLRDCMAF GDAMNDREML GSVGSGFIMG NAMPQLRAEL
     PHLPVIGHCR NQAVSHYLTH WLDYPHLPYS PE
//

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