(data stored in ACNUC10821 zone)

SWISSPROT: C8U2N3_ECO10

ID   C8U2N3_ECO10            Unreviewed;       129 AA.
AC   C8U2N3;
DT   03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT   03-NOV-2009, sequence version 1.
DT   05-DEC-2018, entry version 50.
DE   RecName: Full=Crossover junction endodeoxyribonuclease rusA {ECO:0000256|PIRNR:PIRNR001007};
DE            EC=3.1.22.4 {ECO:0000256|PIRNR:PIRNR001007};
GN   OrderedLocusNames=ECO103_0524 {ECO:0000313|EMBL:BAI29391.1};
OS   Escherichia coli O103:H2 (strain 12009 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585395 {ECO:0000313|EMBL:BAI29391.1, ECO:0000313|Proteomes:UP000000959};
RN   [1] {ECO:0000313|EMBL:BAI29391.1, ECO:0000313|Proteomes:UP000000959}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12009 / EHEC {ECO:0000313|Proteomes:UP000000959};
RX   PubMed=19815525; DOI=10.1073/pnas.0903585106;
RA   Ogura Y., Ooka T., Iguchi A., Toh H., Asadulghani M., Oshima K.,
RA   Kodama T., Abe H., Nakayama K., Kurokawa K., Tobe T., Hattori M.,
RA   Hayashi T.;
RT   "Comparative genomics reveal the mechanism of the parallel evolution
RT   of O157 and non-O157 enterohemorrhagic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:17939-17944(2009).
CC   -!- FUNCTION: Endonuclease that resolves Holliday junction
CC       intermediates made during homologous genetic recombination and DNA
CC       repair. Exhibits sequence and structure-selective cleavage of
CC       four-way DNA junctions, where it introduces symmetrical nicks in
CC       two strands of the same polarity at the 5' side of dinucleotides.
CC       Corrects the defects in genetic recombination and DNA repair
CC       associated with inactivation of ruvAB or ruvC.
CC       {ECO:0000256|PIRNR:PIRNR001007}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage at a junction such as a
CC         reciprocal single-stranded crossover between two homologous DNA
CC         duplexes (Holliday junction).; EC=3.1.22.4;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001007};
CC   -!- SIMILARITY: Belongs to the rusA family.
CC       {ECO:0000256|PIRNR:PIRNR001007}.
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DR   EMBL; AP010958; BAI29391.1; -; Genomic_DNA.
DR   RefSeq; WP_000767117.1; NC_013353.1.
DR   EnsemblBacteria; BAI29391; BAI29391; ECO103_0524.
DR   KEGG; eoh:ECO103_0524; -.
DR   HOGENOM; HOG000119986; -.
DR   KO; K01160; -.
DR   OMA; NSYWRAP; -.
DR   BioCyc; ECOL585395:ECO103_RS02735-MONOMER; -.
DR   Proteomes; UP000000959; Chromosome.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1330.70; -; 1.
DR   InterPro; IPR016281; Endonuclease_RusA.
DR   InterPro; IPR008822; Endonuclease_RusA-like.
DR   InterPro; IPR036614; RusA-like_sf.
DR   Pfam; PF05866; RusA; 1.
DR   PIRSF; PIRSF001007; RusA; 1.
DR   SUPFAM; SSF103084; SSF103084; 1.
PE   3: Inferred from homology;
DR   PRODOM; C8U2N3.
DR   SWISS-2DPAGE; C8U2N3.
KW   Complete proteome {ECO:0000313|Proteomes:UP000000959};
KW   DNA damage {ECO:0000256|PIRNR:PIRNR001007};
KW   DNA repair {ECO:0000256|PIRNR:PIRNR001007};
KW   Endonuclease {ECO:0000256|PIRNR:PIRNR001007};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001007};
KW   Nuclease {ECO:0000256|PIRNR:PIRNR001007}.
SQ   SEQUENCE   129 AA;  14172 MW;  D18FD0D2A6C930EF CRC64;
     MKLILPFPPS VNTYWRHPNK GAFAGKSLIS AAGRKFQSAA CAAIVEQLRR LPKPTSAPAS
     VEIVLFPPDN RIRDLDNYNK ALFDALTHAG VWEDDSQVKR MLVEWGPVIP KGKVEITISK
     YEKTAGAAA
//

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