(data stored in ACNUC7421 zone)

SWISSPROT: D4YX16_SPHJU

ID   D4YX16_SPHJU            Unreviewed;       275 AA.
AC   D4YX16;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   07-JUN-2017, entry version 51.
DE   RecName: Full=Release factor glutamine methyltransferase {ECO:0000256|HAMAP-Rule:MF_02126};
DE            Short=RF MTase {ECO:0000256|HAMAP-Rule:MF_02126};
DE            EC=2.1.1.297 {ECO:0000256|HAMAP-Rule:MF_02126};
DE   AltName: Full=N5-glutamine methyltransferase PrmC {ECO:0000256|HAMAP-Rule:MF_02126};
DE   AltName: Full=Protein-(glutamine-N5) MTase PrmC {ECO:0000256|HAMAP-Rule:MF_02126};
DE   AltName: Full=Protein-glutamine N-methyltransferase PrmC {ECO:0000256|HAMAP-Rule:MF_02126};
GN   Name=hemK {ECO:0000313|EMBL:BAI94898.1};
GN   Synonyms=prmC {ECO:0000256|HAMAP-Rule:MF_02126};
GN   OrderedLocusNames=SJA_C1-00640 {ECO:0000313|EMBL:BAI94898.1};
OS   Sphingobium japonicum (strain NBRC 101211 / UT26S).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=452662 {ECO:0000313|EMBL:BAI94898.1, ECO:0000313|Proteomes:UP000007753};
RN   [1] {ECO:0000313|EMBL:BAI94898.1, ECO:0000313|Proteomes:UP000007753}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101211 / UT26S {ECO:0000313|Proteomes:UP000007753};
RX   PubMed=20817768; DOI=10.1128/JB.00961-10;
RA   Nagata Y., Ohtsubo Y., Endo R., Ichikawa N., Ankai A., Oguchi A.,
RA   Fukui S., Fujita N., Tsuda M.;
RT   "Complete genome sequence of the representative gamma-
RT   hexachlorocyclohexane-degrading bacterium Sphingobium japonicum
RT   UT26.";
RL   J. Bacteriol. 192:5852-5853(2010).
CC   -!- FUNCTION: Methylates the class 1 translation termination release
CC       factors RF1/PrfA and RF2/PrfB on the glutamine residue of the
CC       universally conserved GGQ motif. {ECO:0000256|HAMAP-
CC       Rule:MF_02126}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + [peptide chain
CC       release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine +
CC       [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
CC       {ECO:0000256|HAMAP-Rule:MF_02126}.
CC   -!- SIMILARITY: Belongs to the protein N5-glutamine methyltransferase
CC       family. PrmC subfamily. {ECO:0000256|HAMAP-Rule:MF_02126}.
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DR   EMBL; AP010803; BAI94898.1; -; Genomic_DNA.
DR   RefSeq; WP_013038736.1; NC_014006.1.
DR   ProteinModelPortal; D4YX16; -.
DR   STRING; 452662.SJA_C1-00640; -.
DR   EnsemblBacteria; BAI94898; BAI94898; SJA_C1-00640.
DR   GeneID; 29271772; -.
DR   KEGG; sjp:SJA_C1-00640; -.
DR   eggNOG; ENOG4105EQY; Bacteria.
DR   eggNOG; COG2890; LUCA.
DR   HOGENOM; HOG000076274; -.
DR   KO; K02493; -.
DR   OMA; MLVSNPP; -.
DR   OrthoDB; POG091H02CJ; -.
DR   Proteomes; UP000007753; Chromosome 1.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0036009; F:protein-glutamine N-methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0018364; P:peptidyl-glutamine methylation; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_02126; RF_methyltr_PrmC; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR025714; Methyltranfer_dom.
DR   InterPro; IPR004556; Modification_methylase_HemK.
DR   InterPro; IPR019874; Release_fac_Glu-N5_MeTfrase.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   PANTHER; PTHR18895:SF83; PTHR18895:SF83; 1.
DR   Pfam; PF13847; Methyltransf_31; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR00536; hemK_fam; 1.
DR   TIGRFAMs; TIGR03534; RF_mod_PrmC; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
DR   PRODOM; D4YX16.
DR   SWISS-2DPAGE; D4YX16.
KW   Complete proteome {ECO:0000313|Proteomes:UP000007753};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_02126,
KW   ECO:0000256|SAAS:SAAS00089528, ECO:0000313|EMBL:BAI94898.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007753};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_02126,
KW   ECO:0000256|SAAS:SAAS00461716};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_02126,
KW   ECO:0000256|SAAS:SAAS00089490, ECO:0000313|EMBL:BAI94898.1}.
FT   DOMAIN      108    234       Methyltranfer_dom. {ECO:0000259|Pfam:
FT                                PF13847}.
FT   REGION      116    120       S-adenosyl-L-methionine binding.
FT                                {ECO:0000256|HAMAP-Rule:MF_02126}.
FT   REGION      182    185       Substrate binding. {ECO:0000256|HAMAP-
FT                                Rule:MF_02126}.
FT   BINDING     139    139       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_02126}.
FT   BINDING     168    168       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_02126}.
FT   BINDING     182    182       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_02126}.
SQ   SEQUENCE   275 AA;  28988 MW;  5DA71A8030E5B390 CRC64;
     MGVAVALREA VERLTPISAT PRLDAELLLA HALRIDRNTL LLRQRDLSVP PGFDALIQRR
     LTGEPIAYIT GTRDFWTISL HVTPDVLIPR PDSETLIEAA LDHFGPRSPA RILDLGTGSG
     ALLLAALSQW PQATGTGVDI SPAAVAVAQG NADRLGLGGR ANFRLGDWAE GMGGPFDLIL
     VNPPYIARDA ALAGDVLHEP ETALFAGAEG LDDYRRIAPA LPRLLAPGGM AAMEIGHDQR
     LSVSALLADQ GLGVSPRRDL AGHDRCLVAT PGPSA
//

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