(data stored in SCRATCH zone)

SWISSPROT: D4YXK7_SPHJU

ID   D4YXK7_SPHJU            Unreviewed;       140 AA.
AC   D4YXK7;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   08-MAY-2019, entry version 50.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase H {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS01091420};
DE            EC=2.1.1.177 {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS01091423};
DE   AltName: Full=23S rRNA (pseudouridine1915-N3)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00658};
DE   AltName: Full=23S rRNA m3Psi1915 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00658};
DE   AltName: Full=rRNA (pseudouridine-N3-)-methyltransferase RlmH {ECO:0000256|HAMAP-Rule:MF_00658};
GN   Name=ybeA {ECO:0000313|EMBL:BAI95089.1};
GN   Synonyms=rlmH {ECO:0000256|HAMAP-Rule:MF_00658};
GN   OrderedLocusNames=SJA_C1-02550 {ECO:0000313|EMBL:BAI95089.1};
OS   Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26
OS   / NBRC 101211 / UT26S).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=452662 {ECO:0000313|EMBL:BAI95089.1, ECO:0000313|Proteomes:UP000007753};
RN   [1] {ECO:0000313|EMBL:BAI95089.1, ECO:0000313|Proteomes:UP000007753}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S
RC   {ECO:0000313|Proteomes:UP000007753};
RX   PubMed=20817768; DOI=10.1128/JB.00961-10;
RA   Nagata Y., Ohtsubo Y., Endo R., Ichikawa N., Ankai A., Oguchi A.,
RA   Fukui S., Fujita N., Tsuda M.;
RT   "Complete genome sequence of the representative gamma-
RT   hexachlorocyclohexane-degrading bacterium Sphingobium japonicum
RT   UT26.";
RL   J. Bacteriol. 192:5852-5853(2010).
CC   -!- FUNCTION: Specifically methylates the pseudouridine at position
CC       1915 (m3Psi1915) in 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00658,
CC       ECO:0000256|SAAS:SAAS01091432}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine
CC         = H(+) + N(3)-methylpseudouridine(1915) in 23S rRNA + S-
CC         adenosyl-L-homocysteine; Xref=Rhea:RHEA:42752, Rhea:RHEA-
CC         COMP:10221, Rhea:RHEA-COMP:10222, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:74486; EC=2.1.1.177; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00658, ECO:0000256|SAAS:SAAS01125024};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00658,
CC       ECO:0000256|SAAS:SAAS01091414}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00658,
CC       ECO:0000256|SAAS:SAAS01091434}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase RlmH family.
CC       {ECO:0000256|HAMAP-Rule:MF_00658, ECO:0000256|SAAS:SAAS01091436}.
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DR   EMBL; AP010803; BAI95089.1; -; Genomic_DNA.
DR   RefSeq; WP_007686702.1; NC_014006.1.
DR   STRING; 452662.SJA_C1-02550; -.
DR   EnsemblBacteria; BAI95089; BAI95089; SJA_C1-02550.
DR   GeneID; 29271958; -.
DR   KEGG; sjp:SJA_C1-02550; -.
DR   eggNOG; ENOG4108UXA; Bacteria.
DR   eggNOG; COG1576; LUCA.
DR   HOGENOM; HOG000218433; -.
DR   KO; K00783; -.
DR   OMA; NGEPYHK; -.
DR   Proteomes; UP000007753; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0070038; F:rRNA (pseudouridine-N3-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_00658; 23SrRNA_methyltr_H; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR003742; RlmH-like.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR33603; PTHR33603; 1.
DR   Pfam; PF02590; SPOUT_MTase; 1.
DR   PIRSF; PIRSF004505; MT_bac; 2.
DR   SUPFAM; SSF75217; SSF75217; 1.
PE   3: Inferred from homology;
DR   PRODOM; D4YXK7.
DR   SWISS-2DPAGE; D4YXK7.
KW   Complete proteome {ECO:0000313|Proteomes:UP000007753};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00658,
KW   ECO:0000256|SAAS:SAAS01091416};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00658,
KW   ECO:0000256|SAAS:SAAS01091417};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007753};
KW   rRNA processing {ECO:0000256|HAMAP-Rule:MF_00658,
KW   ECO:0000256|SAAS:SAAS01091427};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00658,
KW   ECO:0000256|SAAS:SAAS01091425};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00658,
KW   ECO:0000256|SAAS:SAAS01091419}.
FT   REGION      106    111       S-adenosyl-L-methionine binding.
FT                                {ECO:0000256|HAMAP-Rule:MF_00658}.
FT   BINDING      55     55       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen. {ECO:0000256|HAMAP-Rule:
FT                                MF_00658}.
FT   BINDING      87     87       S-adenosyl-L-methionine; via amide
FT                                nitrogen. {ECO:0000256|HAMAP-Rule:
FT                                MF_00658}.
SQ   SEQUENCE   140 AA;  15757 MW;  FC2996E5DC08A577 CRC64;
     MLLHIIARGK IGRSPEAELV DRYARRLTMP FRITELPDRG GKLPPAPPGT VTVMLDEKGR
     QLSSIDFARR IEGWRDSGKR ECRFLIGAAD GFDDAERDNA DLLIAFGAMT WPHMMARAML
     AEQLWRACSI LAGHPYHREG
//

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