(data stored in SCRATCH zone)

SWISSPROT: D4YXM8_SPHJU

ID   D4YXM8_SPHJU            Unreviewed;       707 AA.
AC   D4YXM8;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   16-JAN-2019, entry version 51.
DE   RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump {ECO:0000256|HAMAP-Rule:MF_01129};
DE            EC=7.1.3.1 {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE            Short=H(+)-PPase {ECO:0000256|HAMAP-Rule:MF_01129};
GN   Name=ppa {ECO:0000313|EMBL:BAI95110.1};
GN   Synonyms=hppA {ECO:0000256|HAMAP-Rule:MF_01129};
GN   OrderedLocusNames=SJA_C1-02760 {ECO:0000313|EMBL:BAI95110.1};
OS   Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26
OS   / NBRC 101211 / UT26S).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=452662 {ECO:0000313|EMBL:BAI95110.1, ECO:0000313|Proteomes:UP000007753};
RN   [1] {ECO:0000313|EMBL:BAI95110.1, ECO:0000313|Proteomes:UP000007753}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S
RC   {ECO:0000313|Proteomes:UP000007753};
RX   PubMed=20817768; DOI=10.1128/JB.00961-10;
RA   Nagata Y., Ohtsubo Y., Endo R., Ichikawa N., Ankai A., Oguchi A.,
RA   Fukui S., Fujita N., Tsuda M.;
RT   "Complete genome sequence of the representative gamma-
RT   hexachlorocyclohexane-degrading bacterium Sphingobium japonicum
RT   UT26.";
RL   J. Bacteriol. 192:5852-5853(2010).
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force. {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate;
CC         Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01129};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01129};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01129}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase
CC       (TC 3.A.10) family. K(+)-insensitive subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01129}.
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DR   EMBL; AP010803; BAI95110.1; -; Genomic_DNA.
DR   RefSeq; WP_007687277.1; NC_014006.1.
DR   STRING; 452662.SJA_C1-02760; -.
DR   EnsemblBacteria; BAI95110; BAI95110; SJA_C1-02760.
DR   GeneID; 29271979; -.
DR   KEGG; sjp:SJA_C1-02760; -.
DR   eggNOG; ENOG4105EAY; Bacteria.
DR   eggNOG; COG3808; LUCA.
DR   HOGENOM; HOG000007098; -.
DR   KO; K15987; -.
DR   OMA; MATTAMQ; -.
DR   Proteomes; UP000007753; Chromosome 1.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0009678; F:hydrogen-translocating pyrophosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:1902600; P:proton transmembrane transport; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01129; PPase_energized_pump; 1.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
DR   PIRSF; PIRSF001265; H+-PPase; 1.
DR   TIGRFAMs; TIGR01104; V_PPase; 1.
PE   3: Inferred from homology;
DR   PRODOM; D4YXM8.
DR   SWISS-2DPAGE; D4YXM8.
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Complete proteome {ECO:0000313|Proteomes:UP000007753};
KW   Hydrogen ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Hydrolase {ECO:0000313|EMBL:BAI95110.1};
KW   Ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007753};
KW   Translocase {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transmembrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01129}.
FT   TRANSMEM      6     26       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM     54     72       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM     78     99       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    119    146       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    158    177       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    235    258       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    264    283       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    295    318       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    338    362       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    383    409       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    415    435       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    478    496       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    516    535       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    589    610       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    616    635       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   SITE        473    473       Determinant of potassium independence.
FT                                {ECO:0000256|HAMAP-Rule:MF_01129}.
SQ   SEQUENCE   707 AA;  72245 MW;  E67F9ABC16D5CB54 CRC64;
     MQIVTIAIGC GLLAVLYGLF TSRQVLAASA GNETMQQIAG AIQEGAKAYL SRQYATIAVV
     GVVVAALVFF FLGATSAVGF LIGAILSGAA GFIGMNISVR ANVRTAEAAR GTLQSGLTVA
     FRAGAITGML VAGLALLAIS VFFWYLTGPA GHAPDDRIVI DSLVALAFGA SLISIFARLG
     GGIFTKAADV GADLVGKVEA GIPEDDPRNP AVIADNVGDN VGDCAGMAAD LFETYVVTVG
     ATMVLTALLV TGVDNAFLMR LMSLPLIVGG VCIITSIIGT YMVRLGASQS IMGALYKGFW
     TTAILSIPAL YAVTRFALGD LDARFGADLD GAGGYTGMAL FWAMMVGLAV TGLIVWITEY
     YTGTNYRPVR SIARASETGH GTNVIQGLAV SLEATALPTL VIVAGIIVAH QLAGLIGIAF
     AATAMLALAG MVVALDAYGP VTDNAGGIAE MSHLDDSVRV KTDALDAVGN TTKAVTKGYA
     IGSAGLAALV LFGAYTEDLK FYNSALGLTE PVDFSLSNPY VIVGLLLGAL LPYLFGAMGM
     TAVGRAAGDV VKDVREQFAT NKGIMEGTSR PNYARTVDLV TRAAIKEMIV PSLLPVLAPI
     VVYFAISAVA GTPNGFAALG ALLLGVIVSG LFVALSMTSG GGAWDNAKKY IEDGHHGGKG
     SEAHKAAVTG DTVGDPYKDT AGPAVNPMIK ITNIVALLLL AALAHGA
//

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