(data stored in ACNUC27125 zone)

EMBL: BC025714

ID   BC025714; SV 1; linear; mRNA; STD; HUM; 3307 BP.
XX
AC   BC025714;
XX
DT   13-MAR-2002 (Rel. 71, Created)
DT   15-OCT-2008 (Rel. 97, Last updated, Version 8)
XX
DE   Homo sapiens protein phosphatase 3 (formerly 2B), catalytic subunit, alpha
DE   isoform, mRNA (cDNA clone MGC:34395 IMAGE:5227691), complete cds.
XX
KW   MGC.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-3307
RX   DOI; 10.1073/pnas.242603899.
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
XX
RN   [2]
RC   NIH-MGC Project URL: http://mgc.nci.nih.gov
RP   1-3307
RG   NIH MGC Project
RA   ;
RT   ;
RL   Submitted (06-MAR-2002) to the INSDC.
RL   National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda,
RL   MD 20892-2590, USA
XX
DR   MD5; 99c41a8073b32ea83735baaaf9da4e6f.
DR   Ensembl-Gn; ENSG00000138814; homo_sapiens.
DR   Ensembl-Tr; ENST00000323055; homo_sapiens.
DR   Ensembl-Tr; ENST00000394853; homo_sapiens.
DR   Ensembl-Tr; ENST00000394854; homo_sapiens.
DR   Ensembl-Tr; ENST00000512215; homo_sapiens.
XX
CC   Contact: MGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Life Technologies, Inc.
CC   cDNA Library Preparation: Life Technologies, Inc.
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: National Institutes of Health Intramural
CC   Sequencing Center (NISC),
CC   Gaithersburg, Maryland;
CC   Web site: http://www.nisc.nih.gov/
CC   Contact: nisc_mgc@nhgri.nih.gov
CC   Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
CC   Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
CC   Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
CC   Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
CC   Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
CC   McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
CC   Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
CC   Young,A., Zhang,L.-H. and Green,E.D.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 49 Row: o Column: 5
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 19923130.
CC   Differences found between this sequence and the human reference
CC   genome (build 36) are described in misc_difference features below
CC   and these differences were also compared to chimpanzee genome
CC   (build 1).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..3307
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NIH_MGC_120"
FT                   /clone="MGC:34395 IMAGE:5227691"
FT                   /tissue_type="Pancreas, Spleen, adult pooled"
FT                   /note="Vector: pCMV-SPORT6"
FT                   /db_xref="taxon:9606"
FT   gene            1..3307
FT                   /gene="PPP3CA"
FT                   /note="synonyms: CNA1, PPP2B, CALNA1, CCN1"
FT   CDS_pept        110..1645
FT                   /codon_start=1
FT                   /gene="PPP3CA"
FT                   /product="PPP3CA protein"
FT                   /db_xref="GOA:Q08209"
FT                   /db_xref="H-InvDB:HIT000039980.17"
FT                   /db_xref="HGNC:HGNC:9314"
FT                   /db_xref="InterPro:IPR004843"
FT                   /db_xref="InterPro:IPR006186"
FT                   /db_xref="InterPro:IPR029052"
FT                   /db_xref="InterPro:IPR041751"
FT                   /db_xref="PDB:1AUI"
FT                   /db_xref="PDB:1M63"
FT                   /db_xref="PDB:1MF8"
FT                   /db_xref="PDB:2JOG"
FT                   /db_xref="PDB:2JZI"
FT                   /db_xref="PDB:2P6B"
FT                   /db_xref="PDB:2R28"
FT                   /db_xref="PDB:2W73"
FT                   /db_xref="PDB:3LL8"
FT                   /db_xref="PDB:4F0Z"
FT                   /db_xref="PDB:4Q5U"
FT                   /db_xref="PDB:5C1V"
FT                   /db_xref="PDB:5SVE"
FT                   /db_xref="PDB:6NUC"
FT                   /db_xref="PDB:6NUF"
FT                   /db_xref="PDB:6NUU"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q08209"
FT                   /protein_id="AAH25714.1"
FT                   /translation="MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDI
FT                   LKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEV
FT                   GGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFK
FT                   QECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPP
FT                   AYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAH
FT                   EAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHP
FT                   YWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNK
FT                   IRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKIT
FT                   SFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ"
FT   misc_difference 2383
FT                   /gene="PPP3CA"
FT                   /note="1 base in cDNA is not found in the human genome. The
FT                   chimpanzee genome agrees with the human genomic sequence
FT                   and not the cDNA."
FT   misc_difference 2566
FT                   /gene="PPP3CA"
FT                   /note="1 base in cDNA is not found in the human genome. The
FT                   chimpanzee genome agrees with the cDNA sequence, suggesting
FT                   that this difference is unlikely to be due to an artifact."
FT   misc_difference 2767
FT                   /gene="PPP3CA"
FT                   /note="'T' in cDNA is 'G' in the human genome. The
FT                   chimpanzee genome agrees with the human genomic sequence
FT                   and not the cDNA."
FT   misc_difference 3185..3307
FT                   /gene="PPP3CA"
FT                   /note="polyA tail: 123 bases do not align to the human
FT                   genome."
XX
SQ   Sequence 3307 BP; 1094 A; 582 C; 674 G; 957 T; 0 other;
     cgcggcggcg gcggcggcgg cggcggcgtt gagtgtctgg cccgccggtc cggtcggggt        60
     gtgcagtcgg acggacgagc agcgcgtcgc tgtcctccgg cagctggaga tgtccgagcc       120
     caaggcaatt gatcccaagt tgtcgacgac cgacagggtg gtgaaagctg ttccatttcc       180
     tccaagtcac cggcttacag caaaagaagt gtttgataat gatggaaaac ctcgtgtgga       240
     tatcttaaag gcgcatctta tgaaggaggg aaggctggaa gagagtgttg cattgagaat       300
     aataacagag ggtgcatcaa ttcttcgaca ggaaaaaaat ttgctggata ttgatgcgcc       360
     agtcactgtt tgtggggaca ttcatggaca attctttgat ttgatgaagc tctttgaagt       420
     cgggggatct cctgccaaca ctcgctacct cttcttaggg gactatgttg acagagggta       480
     cttcagtatt gaatgtgtgc tgtatttgtg ggccttgaaa attctctacc ccaaaacact       540
     gtttttactt cgtggaaatc atgaatgtag acatctaaca gagtatttca catttaaaca       600
     agaatgtaaa ataaagtatt cagaacgcgt atatgatgcc tgtatggatg cctttgactg       660
     ccttcccctg gctgccctga tgaaccaaca gttcctgtgt gtgcatggtg gtttgtctcc       720
     agagattaac actttagatg atatcagaaa attagaccga ttcaaagaac cacctgcata       780
     tggacctatg tgtgatatcc tgtggtcaga ccccctggaa gattttggaa atgagaagac       840
     tcaggaacat ttcactcaca acacagtcag ggggtgttca tacttctaca gttacccggc       900
     tgtatgtgaa ttcttacagc acaataactt gttatctata ctccgagccc acgaagccca       960
     agatgcaggg taccgcatgt acaggaaaag ccaaacaaca ggcttccctt ctctaattac      1020
     aattttttca gcaccaaatt acttagatgt atacaataac aaagctgcag tattgaagta      1080
     tgagaacaat gttatgaata tcaggcaatt caactgttct cctcatccat actggcttcc      1140
     aaatttcatg gatgttttta cttggtccct tccatttgtt ggggaaaaag tgactgagat      1200
     gctggtaaat gtcctcaaca tctgctcaga tgatgaacta gggtcagaag aagatggatt      1260
     tgatggtgca acagctgcag cccggaaaga ggtgataagg aacaagatcc gagcaatagg      1320
     caaaatggcc agagtgttct cagtgctcag agaagagagt gagagtgtgc tgacgctgaa      1380
     aggcttgacc ccaactggca tgctccccag cggagtactt tctggaggga agcaaaccct      1440
     gcaaagcgct atcaaaggat tttcaccaca acataagatc actagcttcg aggaagccaa      1500
     gggcttagac cgaattaatg agaggatgcc gcctcgcaga gatgccatgc cctctgacgc      1560
     caaccttaac tccatcaaca aggctctcac ctcagagact aacggcacgg acagcaatgg      1620
     cagtaatagc agcaatattc agtgaccact tcctgttcac tttttttttt tttttttttt      1680
     tttttttttg agctgcgggg catgatgggg attgctgcat atcagcagtt ggatgttctt      1740
     gcctctgaca gtagcttatt tgctctgggg gccaggaatt ggattcagtt tacactatca      1800
     ttaaaaaaga gggagagaga taataaacta tattttggtg gggatggtga ttaaacacct      1860
     cttttgggta tgccttttaa aaatgcttat agagaaaaaa aattttaaaa agaaagctaa      1920
     tgctagtata tactgcaatg ttaggggaat gaacatgttt tcctactgca ttggggactt      1980
     ctagataggt taatgaaagg ccttttattc tgttactgga catgaaaact ttgtctaatt      2040
     tcttactcta ttgtacgttt acagtcgcag cactaaaaat ggatgacatc aaacattttt      2100
     aacaaaatga tgtacaaact aaggactatt tattgataat gttttgctac tcttgtcaga      2160
     caatggctat aaactgaatt aggcagtctt aaaaaaaaaa aaaaacagaa aaagaaaaaa      2220
     aagaacgttg caaatttgtt aaaatgccaa aaaggacagt ttaattttgt acagattatg      2280
     cttacctcag gtttctttag tgtgcttgaa tgcccttctt tccatataac acttatcttc      2340
     ttcttaattc ggcaatggaa tatcttttaa gttttaaaaa aaactggaat aattatatct      2400
     atcttttttg ccgtttatat ttaggggttt ttgttgataa aatcaagtct tggttgtggc      2460
     ttgctgaatt aaatatttat gagtggtgca tttttaagta tagtgaacaa gacaccatat      2520
     taagtacagt gataaagcat ctatattctg taaaaaaaaa aaaaaatctg cctatgcatg      2580
     ttttttaaga aaaaaaaaat ggctgtatcg gcctgtatgg gactgtaatg cgcttagtgg      2640
     tctgacatat actggaaatg tatgtatact ggcgtacttt atattctcta aaatgcttaa      2700
     tgcctttgaa attttgtaat caaaaaaaag ctttgaaaaa atctaaaggg gagagtattc      2760
     tttaaatttt ttaacataag cttgtcaatg cacatgtaga tggttagcat gtttagcaaa      2820
     ccttgtgaaa ttataataag tttgtagtta catgtgaaac tctaaatgca tggcaactgt      2880
     taatgtcata acagtttagt tattttgttc tgttctgtca tgtgccacaa aatatgtact      2940
     tttttcactt ttttcccttt gtatatcagt tacgggttac aactggttca ttctgaaaac      3000
     aacaacaaca aaagtccatt catatttttt aacaattgta taagtgccca agtaattcac      3060
     tacagcctaa agccttgcct ttgtaatttg acttctgaca tgttggcaat caaagcatgc      3120
     acttgtaaca atgaaaaaga aaaagcattt tatattacta ctcaataaaa tgtgcatgaa      3180
     cttaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      3240
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      3300
     aaaaaaa                                                                3307
//

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