(data stored in SCRATCH zone)

SWISSPROT: C0ZM61_RHOE4

ID   C0ZM61_RHOE4            Unreviewed;       153 AA.
AC   C0ZM61;
DT   26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT   26-MAY-2009, sequence version 1.
DT   08-MAY-2019, entry version 56.
DE   RecName: Full=tRNA-specific adenosine deaminase {ECO:0000256|HAMAP-Rule:MF_00972};
DE            EC=3.5.4.33 {ECO:0000256|HAMAP-Rule:MF_00972};
GN   Name=tadA {ECO:0000256|HAMAP-Rule:MF_00972,
GN   ECO:0000313|EMBL:BAH31038.1};
GN   OrderedLocusNames=RER_03300 {ECO:0000313|EMBL:BAH31038.1};
OS   Rhodococcus erythropolis (strain PR4 / NBRC 100887).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae;
OC   Rhodococcus.
OX   NCBI_TaxID=234621 {ECO:0000313|EMBL:BAH31038.1, ECO:0000313|Proteomes:UP000002204};
RN   [1] {ECO:0000313|Proteomes:UP000002204}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PR4 / NBRC 100887 {ECO:0000313|Proteomes:UP000002204};
RA   Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S.,
RA   Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.;
RT   "Comparison of the complete genome sequences of Rhodococcus
RT   erythropolis PR4 and Rhodococcus opacus B4.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:BAH31038.1, ECO:0000313|Proteomes:UP000002204}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PR4 / NBRC 100887 {ECO:0000313|Proteomes:UP000002204};
RX   PubMed=16423019; DOI=10.1111/j.1462-2920.2005.00899.x;
RA   Sekine M., Tanikawa S., Omata S., Saito M., Fujisawa T., Tsukatani N.,
RA   Tajima T., Sekigawa T., Kosugi H., Matsuo Y., Nishiko R., Imamura K.,
RA   Ito M., Narita H., Tago S., Fujita N., Harayama S.;
RT   "Sequence analysis of three plasmids harboured in Rhodococcus
RT   erythropolis strain PR4.";
RL   Environ. Microbiol. 8:334-346(2006).
CC   -!- FUNCTION: Catalyzes the deamination of adenosine to inosine at the
CC       wobble position 34 of tRNA(Arg2). {ECO:0000256|HAMAP-
CC       Rule:MF_00972}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA
CC         + NH4(+); Xref=Rhea:RHEA:43168, Rhea:RHEA-COMP:10373, Rhea:RHEA-
CC         COMP:10374, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:74411, ChEBI:CHEBI:82852;
CC         EC=3.5.4.33; Evidence={ECO:0000256|HAMAP-Rule:MF_00972};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00972};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00972};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00972}.
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DR   EMBL; AP008957; BAH31038.1; -; Genomic_DNA.
DR   RefSeq; WP_020905862.1; NC_012490.1.
DR   STRING; 234621.RER_03300; -.
DR   EnsemblBacteria; BAH31038; BAH31038; RER_03300.
DR   KEGG; rer:RER_03300; -.
DR   PATRIC; fig|234621.6.peg.766; -.
DR   eggNOG; ENOG4108Z6B; Bacteria.
DR   eggNOG; COG0590; LUCA.
DR   HOGENOM; HOG000085050; -.
DR   KO; K11991; -.
DR   OMA; LEPCQMC; -.
DR   Proteomes; UP000002204; Chromosome.
DR   GO; GO:0052717; F:tRNA-specific adenosine-34 deaminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00972; tRNA_aden_deaminase; 1.
DR   InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR   InterPro; IPR002125; CMP_dCMP_dom.
DR   InterPro; IPR016193; Cytidine_deaminase-like.
DR   InterPro; IPR028883; tRNA_aden_deaminase.
DR   Pfam; PF00383; dCMP_cyt_deam_1; 1.
DR   SUPFAM; SSF53927; SSF53927; 1.
DR   PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR   PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C0ZM61.
DR   SWISS-2DPAGE; C0ZM61.
KW   Complete proteome {ECO:0000313|Proteomes:UP000002204};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00972,
KW   ECO:0000313|EMBL:BAH31038.1};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00972};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   DOMAIN        4    116       CMP/dCMP-type deaminase.
FT                                {ECO:0000259|PROSITE:PS51747}.
FT   ACT_SITE     57     57       Proton donor. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        55     55       Zinc; via pros nitrogen; catalytic.
FT                                {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   METAL        86     86       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        89     89       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
SQ   SEQUENCE   153 AA;  16285 MW;  DDE2DDFEB13F20B1 CRC64;
     MTLADDQRMI RAAIVAARSA SDADVPVGAV VFDADGVEVA RAANAREAAS DPTAHAEILA
     LRAAAKIYGD GWRLEGATLA VTLEPCTMCA GALVLARVSR VVFGAWEPKT GAVGSLWDVV
     RDKRLTYRPQ VRGGVLEDEC AGLLEDFFRE QRA
//

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