(data stored in SCRATCH zone)

SWISSPROT: C3MGU8_SINFN

ID   C3MGU8_SINFN            Unreviewed;       150 AA.
AC   C3MGU8;
DT   16-JUN-2009, integrated into UniProtKB/TrEMBL.
DT   16-JUN-2009, sequence version 1.
DT   07-JUN-2017, entry version 52.
DE   RecName: Full=tRNA-specific adenosine deaminase {ECO:0000256|HAMAP-Rule:MF_00972};
DE            EC=3.5.4.33 {ECO:0000256|HAMAP-Rule:MF_00972};
GN   Name=tadA {ECO:0000256|HAMAP-Rule:MF_00972};
GN   OrderedLocusNames=NGR_c04170 {ECO:0000313|EMBL:ACP24213.1};
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394 {ECO:0000313|EMBL:ACP24213.1, ECO:0000313|Proteomes:UP000001054};
RN   [1] {ECO:0000313|EMBL:ACP24213.1, ECO:0000313|Proteomes:UP000001054}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234 {ECO:0000313|Proteomes:UP000001054};
RX   PubMed=19376903; DOI=10.1128/AEM.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of
RT   secretion systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Catalyzes the deamination of adenosine to inosine at the
CC       wobble position 34 of tRNA(Arg2). {ECO:0000256|HAMAP-
CC       Rule:MF_00972}.
CC   -!- CATALYTIC ACTIVITY: Adenine(34) in tRNA + H(2)O = hypoxanthine(34)
CC       in tRNA + NH(3). {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00972};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_00972};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00972}.
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family. {ECO:0000256|HAMAP-Rule:MF_00972}.
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DR   EMBL; CP001389; ACP24213.1; -; Genomic_DNA.
DR   RefSeq; WP_012706998.1; NC_012587.1.
DR   RefSeq; YP_002824966.1; NC_012587.1.
DR   ProteinModelPortal; C3MGU8; -.
DR   STRING; 394.NGR_c04170; -.
DR   EnsemblBacteria; ACP24213; ACP24213; NGR_c04170.
DR   GeneID; 7791643; -.
DR   KEGG; rhi:NGR_c04170; -.
DR   PATRIC; fig|394.7.peg.3223; -.
DR   eggNOG; ENOG4108Z6B; Bacteria.
DR   eggNOG; COG0590; LUCA.
DR   HOGENOM; HOG000085050; -.
DR   OMA; LEPCQMC; -.
DR   OrthoDB; POG091H01W1; -.
DR   Proteomes; UP000001054; Chromosome.
DR   GO; GO:0052717; F:tRNA-specific adenosine-34 deaminase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00972; tRNA_aden_deaminase; 1.
DR   InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR   InterPro; IPR002125; CMP_dCMP_dom.
DR   InterPro; IPR016193; Cytidine_deaminase-like.
DR   InterPro; IPR028883; tRNA_aden_deaminase.
DR   Pfam; PF14437; MafB19-deam; 1.
DR   SUPFAM; SSF53927; SSF53927; 1.
DR   PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR   PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE   3: Inferred from homology;
DR   PRODOM; C3MGU8.
DR   SWISS-2DPAGE; C3MGU8.
KW   Complete proteome {ECO:0000313|Proteomes:UP000001054};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001054};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00972};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   DOMAIN        3    112       CMP/dCMP-type deaminase.
FT                                {ECO:0000259|PROSITE:PS51747}.
FT   ACT_SITE     56     56       Proton donor. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        54     54       Zinc; via pros nitrogen; catalytic.
FT                                {ECO:0000256|HAMAP-Rule:MF_00972}.
FT   METAL        84     84       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
FT   METAL        87     87       Zinc; catalytic. {ECO:0000256|HAMAP-Rule:
FT                                MF_00972}.
SQ   SEQUENCE   150 AA;  16117 MW;  475407BFF115A7A8 CRC64;
     MMAETTRYMD AALDEARKAA ARGEVPIGAV VVLDGEVVAA AGNRTRELRD ITAHAEIEAI
     RQAAAAVGDE RLSGADLYVT LEPCTMCAAA ISFARIRRLY YGAEDPKGGA VENGVRFYGS
     PTCHHVPDVY SGLAEREAAD LLRAFFTARR
//

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