(data stored in SCRATCH zone)

SWISSPROT: C5BBB0_EDWI9

ID   C5BBB0_EDWI9            Unreviewed;       675 AA.
AC   C5BBB0;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2012, sequence version 2.
DT   08-MAY-2019, entry version 74.
DE   RecName: Full=ATP-dependent DNA helicase Rep {ECO:0000256|HAMAP-Rule:MF_01920};
DE            EC=3.6.4.12 {ECO:0000256|HAMAP-Rule:MF_01920};
GN   Name=rep {ECO:0000256|HAMAP-Rule:MF_01920};
GN   OrderedLocusNames=NT01EI_0085 {ECO:0000313|EMBL:ACR67345.2};
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503 {ECO:0000313|EMBL:ACR67345.2, ECO:0000313|Proteomes:UP000001485};
RN   [1] {ECO:0000313|Proteomes:UP000001485}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146 {ECO:0000313|Proteomes:UP000001485};
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ACR67345.2, ECO:0000313|Proteomes:UP000001485}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146 {ECO:0000313|EMBL:ACR67345.2,
RC   ECO:0000313|Proteomes:UP000001485};
RX   PubMed=22247535; DOI=10.1128/JB.06522-11;
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Banes M.M.,
RA   Lawrence M.L.;
RT   "Genome Sequence of Edwardsiella ictaluri 93-146, a Strain Associated
RT   with a Natural Channel Catfish Outbreak of Enteric Septicemia of
RT   Catfish.";
RL   J. Bacteriol. 194:740-741(2012).
CC   -!- FUNCTION: Rep helicase is a single-stranded DNA-dependent ATPase
CC       involved in DNA replication; it can initiate unwinding at a nick
CC       in the DNA. It binds to the single-stranded DNA and acts in a
CC       progressive fashion along the DNA in the 3' to 5' direction.
CC       {ECO:0000256|HAMAP-Rule:MF_01920}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01920,
CC         ECO:0000256|SAAS:SAAS01129065};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01920}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_01920, ECO:0000256|SAAS:SAAS00709641}.
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DR   EMBL; CP001600; ACR67345.2; -; Genomic_DNA.
DR   RefSeq; WP_015869566.1; NC_012779.2.
DR   EnsemblBacteria; ACR67345; ACR67345; NT01EI_0085.
DR   GeneID; 7959804; -.
DR   KEGG; eic:NT01EI_0085; -.
DR   PATRIC; fig|634503.3.peg.79; -.
DR   eggNOG; ENOG4105C4R; Bacteria.
DR   eggNOG; COG0210; LUCA.
DR   HOGENOM; HOG000033015; -.
DR   KO; K03656; -.
DR   OrthoDB; 137860at2; -.
DR   BioCyc; EICT634503:G1GVC-78-MONOMER; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.160; -; 1.
DR   HAMAP; MF_01920; Helicase_Rep; 1.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR005752; Helicase_Rep.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01074; rep; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5BBB0.
DR   SWISS-2DPAGE; C5BBB0.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01920,
KW   ECO:0000256|SAAS:SAAS00146071}; Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001485};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_01920};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_01920,
KW   ECO:0000256|SAAS:SAAS00745286};
KW   Helicase {ECO:0000256|HAMAP-Rule:MF_01920,
KW   ECO:0000256|SAAS:SAAS00553650, ECO:0000313|EMBL:ACR67345.2};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01920,
KW   ECO:0000256|SAAS:SAAS00146062, ECO:0000313|EMBL:ACR67345.2};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01920,
KW   ECO:0000256|SAAS:SAAS00145996};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001485}.
FT   DOMAIN        1    280       UvrD-like helicase ATP-binding.
FT                                {ECO:0000259|PROSITE:PS51198}.
FT   DOMAIN      281    562       UvrD-like helicase C-terminal.
FT                                {ECO:0000259|PROSITE:PS51217}.
FT   COILED      125    145       {ECO:0000256|SAM:Coils}.
FT   COILED      653    673       {ECO:0000256|SAM:Coils}.
FT   BINDING     278    278       ATP. {ECO:0000256|HAMAP-Rule:MF_01920}.
SQ   SEQUENCE   675 AA;  77283 MW;  652CD0D22ECAB0EA CRC64;
     MRLNPGQQQA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RVCGYQARHI AAVTFTNKAA
     REMKERVAQT LGRKEARGLM ISTFHTLGLE IIKREYKALG MKSNFSLFDD QDQLALLTDL
     TERWLEKDKT QLQQLIATIS NWKNDLIDPA QAAAQARSAR ERLFADCYGL YDTQLKSCNV
     LDFDDLILLP TLLLRRDPEV RERWQQRIRY LLVDEYQDTN TSQYELVKLL VGSRARFTVV
     GDDDQSIYSW RGARPQNLVL LKEDFPALQV VKLEQNYRSS QRILKAANIL IANNPHVFEK
     RLFSELGYGE PLKVITANNE DHEAERVVGE LIAHHFIHKT AYSDYAILYR GNHQSRVFEK
     MLMQNRIPYK ISGGTSFFSR AEIKDLLSYL RVLTNPDDDS AFLRIVNTPR REIGPSTLQK
     LGAWANQRGI SLFRASFDVG LEQTLSGRGL ASLQHFTRWM DELSRLCERD ATAAVRDLIH
     GIDYESWLCE TSASPRAAEM RMKNVNQLFG WMTDMLQGSD LDEPMTLTQV VNRFTLRDMM
     ERGESEEEAD QVQLMTLHAS KGLEFPYVYL VGMEEGLLPH QSSIDEDNVD EERRLAYVGI
     TRAQKALVFT LCRERRQYGE LMRPEPSRFL LELPQDDLSW ESARKVVSPQ ERMQRGQSHL
     ANLKAQLARA KSAQD
//

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