(data stored in SCRATCH zone)

SWISSPROT: C5BF94_EDWI9

ID   C5BF94_EDWI9            Unreviewed;       458 AA.
AC   C5BF94;
DT   28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   28-JUL-2009, sequence version 1.
DT   08-MAY-2019, entry version 67.
DE   RecName: Full=UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase {ECO:0000256|HAMAP-Rule:MF_02020};
DE            EC=6.3.2.45 {ECO:0000256|HAMAP-Rule:MF_02020};
DE   AltName: Full=Murein peptide ligase {ECO:0000256|HAMAP-Rule:MF_02020};
DE   AltName: Full=UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase {ECO:0000256|HAMAP-Rule:MF_02020};
GN   Name=mpl {ECO:0000256|HAMAP-Rule:MF_02020};
GN   OrderedLocusNames=NT01EI_0442 {ECO:0000313|EMBL:ACR67680.1};
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503 {ECO:0000313|EMBL:ACR67680.1, ECO:0000313|Proteomes:UP000001485};
RN   [1] {ECO:0000313|Proteomes:UP000001485}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146 {ECO:0000313|Proteomes:UP000001485};
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ACR67680.1, ECO:0000313|Proteomes:UP000001485}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146 {ECO:0000313|EMBL:ACR67680.1,
RC   ECO:0000313|Proteomes:UP000001485};
RX   PubMed=22247535; DOI=10.1128/JB.06522-11;
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Banes M.M.,
RA   Lawrence M.L.;
RT   "Genome Sequence of Edwardsiella ictaluri 93-146, a Strain Associated
RT   with a Natural Channel Catfish Outbreak of Enteric Septicemia of
RT   Catfish.";
RL   J. Bacteriol. 194:740-741(2012).
CC   -!- FUNCTION: Reutilizes the intact tripeptide L-alanyl-gamma-D-
CC       glutamyl-meso-diaminopimelate by linking it to UDP-N-
CC       acetylmuramate. {ECO:0000256|HAMAP-Rule:MF_02020}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate +
CC         UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-
CC         acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-
CC         diaminopimelate; Xref=Rhea:RHEA:29563, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:61401,
CC         ChEBI:CHEBI:70757, ChEBI:CHEBI:83905, ChEBI:CHEBI:456216;
CC         EC=6.3.2.45; Evidence={ECO:0000256|HAMAP-Rule:MF_02020};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_02020};
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan recycling.
CC       {ECO:0000256|HAMAP-Rule:MF_02020}.
CC   -!- SIMILARITY: Belongs to the MurCDEF family. Mpl subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_02020}.
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DR   EMBL; CP001600; ACR67680.1; -; Genomic_DNA.
DR   RefSeq; WP_015869883.1; NC_012779.2.
DR   EnsemblBacteria; ACR67680; ACR67680; NT01EI_0442.
DR   GeneID; 7961267; -.
DR   KEGG; eic:NT01EI_0442; -.
DR   PATRIC; fig|634503.3.peg.402; -.
DR   eggNOG; ENOG4105DFU; Bacteria.
DR   eggNOG; COG0773; LUCA.
DR   HOGENOM; HOG000256032; -.
DR   KO; K02558; -.
DR   OMA; CDANVYP; -.
DR   OrthoDB; 307881at2; -.
DR   BioCyc; EICT634503:G1GVC-413-MONOMER; -.
DR   UniPathway; UPA00544; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0016881; F:acid-amino acid ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009254; P:peptidoglycan turnover; IEA:UniProtKB-UniRule.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1190.10; -; 1.
DR   Gene3D; 3.90.190.20; -; 1.
DR   HAMAP; MF_02020; Mpl; 1.
DR   InterPro; IPR005757; Mpl.
DR   InterPro; IPR036565; Mur-like_cat_sf.
DR   InterPro; IPR004101; Mur_ligase_C.
DR   InterPro; IPR036615; Mur_ligase_C_dom_sf.
DR   InterPro; IPR013221; Mur_ligase_cen.
DR   InterPro; IPR000713; Mur_ligase_N.
DR   Pfam; PF01225; Mur_ligase; 1.
DR   Pfam; PF02875; Mur_ligase_C; 1.
DR   Pfam; PF08245; Mur_ligase_M; 1.
DR   SUPFAM; SSF53244; SSF53244; 1.
DR   SUPFAM; SSF53623; SSF53623; 1.
DR   TIGRFAMs; TIGR01081; mpl; 1.
PE   3: Inferred from homology;
DR   PRODOM; C5BF94.
DR   SWISS-2DPAGE; C5BF94.
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Cell cycle {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Cell division {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Cell shape {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Cell wall biogenesis/degradation {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001485};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_02020, ECO:0000313|EMBL:ACR67680.1};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Peptidoglycan synthesis {ECO:0000256|HAMAP-Rule:MF_02020};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001485}.
FT   DOMAIN        2    100       Mur_ligase. {ECO:0000259|Pfam:PF01225}.
FT   DOMAIN      108    291       Mur_ligase_M. {ECO:0000259|Pfam:PF08245}.
FT   DOMAIN      312    360       Mur_ligase_C. {ECO:0000259|Pfam:PF02875}.
FT   NP_BIND     110    116       ATP. {ECO:0000256|HAMAP-Rule:MF_02020}.
SQ   SEQUENCE   458 AA;  49915 MW;  7E84CC3FDEE07AA8 CRC64;
     MRIHILGVCG TFMGGLALLA RQLGHDVTGS DANVYPPMST LLQQQGITLL EGYDPAHLEP
     QPDLVIIGNA LARGNPCVET ILERNIPYVS GPQWLHDEVL RHRWVIAIAG THGKTTTAGM
     VNWILESCGY EPGFVIGGVP GNFSVSARLG NSPFFVIEAD EYDCAFFDKR SKFVHYCPRT
     LILNNLEFDH ADIFDDLRAI QRQFHHLVRL VPGKGRIILP AGDASLKQVM AMGCWSEQEL
     CGDEAAWNVT RLSADASHFS VSLDGACVGE VQWTLVGEHN MHNGLMAIAA ARHVGVPPAE
     ACRALGSFIN ARRRLELRGQ AHGVSVYDDF AHHPTAILAT LAALRGKVGG TARILAVLEP
     RSNTMKMGVC KNELAPALGR ADEVYLYQPQ HIPWQVVEVA EACVQPAYWS ADLGTLVEMI
     VKNAQPGDHI LVMSNGGFGD IHDRLLQALA QKSAVHQE
//

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