(data stored in SCRATCH zone)

SWISSPROT: C6WFC1_ACTMD

ID   C6WFC1_ACTMD            Unreviewed;       786 AA.
AC   C6WFC1;
DT   22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   22-SEP-2009, sequence version 1.
DT   07-JUN-2017, entry version 52.
DE   RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump {ECO:0000256|HAMAP-Rule:MF_01129};
DE            EC=3.6.1.1 {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Membrane-bound proton-translocating pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE   AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_01129};
DE            Short=H(+)-PPase {ECO:0000256|HAMAP-Rule:MF_01129};
GN   Name=hppA {ECO:0000256|HAMAP-Rule:MF_01129};
GN   OrderedLocusNames=Amir_0284 {ECO:0000313|EMBL:ACU34253.1};
OS   Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU
OS   3971).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Actinosynnema.
OX   NCBI_TaxID=446462 {ECO:0000313|EMBL:ACU34253.1, ECO:0000313|Proteomes:UP000002213};
RN   [1] {ECO:0000313|EMBL:ACU34253.1, ECO:0000313|Proteomes:UP000002213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971
RC   {ECO:0000313|Proteomes:UP000002213};
RX   PubMed=21304636; DOI=10.4056/sigs.21137;
RA   Land M., Lapidus A., Mayilraj S., Chen F., Copeland A., Del Rio T.G.,
RA   Nolan M., Lucas S., Tice H., Cheng J.F., Chertkov O., Bruce D.,
RA   Goodwin L., Pitluck S., Rohde M., Goker M., Pati A., Ivanova N.,
RA   Mavromatis K., Chen A., Palaniappan K., Hauser L., Chang Y.J.,
RA   Jeffries C.C., Brettin T., Detter J.C., Han C., Chain P.,
RA   Tindall B.J., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Actinosynnema mirum type strain (101).";
RL   Stand. Genomic Sci. 1:46-53(2009).
CC   -!- FUNCTION: Proton pump that utilizes the energy of pyrophosphate
CC       hydrolysis as the driving force for proton movement across the
CC       membrane. Generates a proton motive force. {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- CATALYTIC ACTIVITY: Diphosphate + H(2)O = 2 phosphate.
CC       {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01129};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01129}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|HAMAP-
CC       Rule:MF_01129}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- SIMILARITY: Belongs to the H(+)-translocating pyrophosphatase (TC
CC       3.A.10) family. K(+)-insensitive subfamily. {ECO:0000256|HAMAP-
CC       Rule:MF_01129}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01129}.
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DR   EMBL; CP001630; ACU34253.1; -; Genomic_DNA.
DR   STRING; 446462.Amir_0284; -.
DR   EnsemblBacteria; ACU34253; ACU34253; Amir_0284.
DR   KEGG; ami:Amir_0284; -.
DR   eggNOG; ENOG4105EAY; Bacteria.
DR   eggNOG; COG3808; LUCA.
DR   HOGENOM; HOG000007098; -.
DR   KO; K15987; -.
DR   OMA; MATTAMQ; -.
DR   OrthoDB; POG091H0DQP; -.
DR   Proteomes; UP000002213; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009678; F:hydrogen-translocating pyrophosphatase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0015992; P:proton transport; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_01129; PPase_energized_pump; 1.
DR   InterPro; IPR004131; PPase-energised_H-pump.
DR   PANTHER; PTHR31998; PTHR31998; 1.
DR   Pfam; PF03030; H_PPase; 1.
DR   PIRSF; PIRSF001265; H+-PPase; 1.
DR   TIGRFAMs; TIGR01104; V_PPase; 1.
PE   3: Inferred from homology;
DR   PRODOM; C6WFC1.
DR   SWISS-2DPAGE; C6WFC1.
KW   Cell membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002213};
KW   Hydrogen ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01129,
KW   ECO:0000313|EMBL:ACU34253.1};
KW   Ion transport {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Membrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002213};
KW   Transmembrane {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_01129};
KW   Transport {ECO:0000256|HAMAP-Rule:MF_01129}.
FT   TRANSMEM     20     38       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM     70     87       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM     99    119       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    144    172       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    178    195       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    251    269       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    275    295       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    307    329       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    349    374       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    395    422       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    428    451       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    496    513       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    533    554       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    607    625       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    631    650       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    702    721       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   TRANSMEM    727    745       Helical. {ECO:0000256|HAMAP-Rule:
FT                                MF_01129}.
FT   SITE        491    491       Determinant of potassium independence.
FT                                {ECO:0000256|HAMAP-Rule:MF_01129}.
SQ   SEQUENCE   786 AA;  79002 MW;  D668E3CE088654E6 CRC64;
     MSRQFLAEGI ELSGGDRGLV AAVAVVALAA LVVGAVLLKE VLAAGQGTAK MQDIAKAVQE
     GASAYLNRQF RTLGIFVVVV FALLFLLPAE DTGERIGRSL FFIVGAVFSA TIGYLGMWLS
     TRANVRVAAA AQAGQSGREK AMRVAFRTGG VVGMFTVGLG LFGAAVVVLV YAGQAPRVLE
     GFGFGAALLA MFMRVGGGIF TKAADVGADL VGKVEQNIPE DDPRNAATIA DNVGDNVGDC
     AGMAADLFES YAVTLVASLI LGTAAFGSQG LLFPLIVPAI GVVTAVIGVY ITGARPGESG
     LSAINRSFYI SAVISAVLCT AAAFAFLPGT FAELGGVSAE IAATEGNPAV IAAAAVLIGI
     VLAGVILWLT GYYTGTEHKP VQEVGRTSLT GAATVILSGI SLGFESAVYT ALVIGGAVYG
     AFLLSGSVIV ALFAVALAGC GLLTTVGVIV AMDTFGPVSD NAQGIAEMSG DVDGEGAQVL
     TELDAVGNTT KAITKGIAIA TAVLAAAALF GSYKDAIEKA LVEVGGVFEA QDFVAFTPNV
     LVGLVIGAAV VFMFSGLAVN AVTRAAGAIV FEVRRQFRDN PGIMDGTTKP EYGRVVDICT
     RDSLRELATP GLLAVMAPIA VGFGLGVGPL AGYLAGAIAT GTLMAVFLAN SGGAWDNAKK
     LVEDGHHGGK GSDAHAATVI GDTVGDPFKD TAGPAINPLI KVMNLVSVLI APAVVTFSVG
     ADASAPVRYG IAVFAVGVVV AAVVVSKRRG TVIGDEPADP PSGSPSVPAQ ATVTDPVQAR
     ATGGTA
//

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