(data stored in SCRATCH zone)

SWISSPROT: PURR_BACSU

ID   PURR_BACSU              Reviewed;         285 AA.
AC   P37551;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   11-DEC-2019, entry version 132.
DE   RecName: Full=Pur operon repressor;
GN   Name=purR; Synonyms=yabI; OrderedLocusNames=BSU00470;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT   chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-25, AND CHARACTERIZATION.
RX   PubMed=7638212; DOI=10.1073/pnas.92.16.7455;
RA   Weng M., Nagy P.L., Zalkin H.;
RT   "Identification of the Bacillus subtilis pur operon repressor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:7455-7459(1995).
CC   -!- FUNCTION: Controls the transcription of the pur operon for purine
CC       biosynthetic genes, binds to the control region of the operon. DNA
CC       binding is inhibited by 5-phosphoribosyl 1-pyrophosphate.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the purine/pyrimidine phosphoribosyltransferase
CC       family. {ECO:0000305}.
DR   EMBL; D26185; BAA05282.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11823.1; -; Genomic_DNA.
DR   PIR; S66076; S66076.
DR   RefSeq; NP_387928.1; NC_000964.3.
DR   RefSeq; WP_003218342.1; NZ_JNCM01000028.1.
DR   PDB; 1O57; X-ray; 2.20 A; A/B/C/D=1-285.
DR   PDB; 1P4A; X-ray; 2.22 A; A/B/C/D=1-285.
DR   PDBsum; 1O57; -.
DR   PDBsum; 1P4A; -.
DR   SMR; P37551; -.
DR   STRING; 224308.BSU00470; -.
DR   DrugBank; DB03942; Carboxylic PRPP.
DR   PaxDb; P37551; -.
DR   PRIDE; P37551; -.
DR   EnsemblBacteria; CAB11823; CAB11823; BSU00470.
DR   GeneID; 937000; -.
DR   KEGG; bsu:BSU00470; -.
DR   PATRIC; fig|224308.179.peg.47; -.
DR   eggNOG; ENOG4105DFT; Bacteria.
DR   eggNOG; COG0503; LUCA.
DR   HOGENOM; HOG000205992; -.
DR   InParanoid; P37551; -.
DR   KO; K09685; -.
DR   OMA; YVSGSQK; -.
DR   PhylomeDB; P37551; -.
DR   BioCyc; BSUB:BSU00470-MONOMER; -.
DR   EvolutionaryTrace; P37551; -.
DR   PRO; PR:P37551; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045982; P:negative regulation of purine nucleobase metabolic process; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0009116; P:nucleoside metabolic process; IEA:InterPro.
DR   CDD; cd06223; PRTases_typeI; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.50.2020; -; 1.
DR   InterPro; IPR000836; PRibTrfase_dom.
DR   InterPro; IPR029057; PRTase-like.
DR   InterPro; IPR015265; PuR_N.
DR   InterPro; IPR010078; PurR_Bsub.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR43864:SF2; PTHR43864:SF2; 1.
DR   Pfam; PF00156; Pribosyltran; 1.
DR   Pfam; PF09182; PuR_N; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF53271; SSF53271; 1.
DR   TIGRFAMs; TIGR01743; purR_Bsub; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P37551.
DR   SWISS-2DPAGE; P37551.
KW   3D-structure; Direct protein sequencing; DNA-binding; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..285
FT                   /note="Pur operon repressor"
FT                   /id="PRO_0000139699"
FT   HELIX           5..17
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           26..32
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           37..53
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          56..62
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          68..73
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           77..91
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           94..96
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   TURN            99..101
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   TURN            106..110
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           112..125
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   TURN            126..128
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          132..137
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   TURN            138..141
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           142..152
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          156..160
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          170..176
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          182..189
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           190..192
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          198..209
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           210..218
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           220..222
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          225..237
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          247..253
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          257..260
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   STRAND          263..266
FT                   /evidence="ECO:0000244|PDB:1O57"
FT   HELIX           269..272
FT                   /evidence="ECO:0000244|PDB:1O57"
SQ   SEQUENCE   285 AA;  31229 MW;  76B1DB289A3F34FB CRC64;
     MKFRRSGRLV DLTNYLLTHP HELIPLTFFS ERYESAKSSI SEDLTIIKQT FEQQGIGTLL
     TVPGAAGGVK YIPKMKQAEA EEFVQTLGQS LANPERILPG GYVYLTDILG KPSVLSKVGK
     LFASVFAERE IDVVMTVATK GIPLAYAAAS YLNVPVVIVR KDNKVTEGST VSINYVSGSS
     NRIQTMSLAK RSMKTGSNVL IIDDFMKAGG TINGMINLLD EFNANVAGIG VLVEAEGVDE
     RLVDEYMSLL TLSTINMKEK SIEIQNGNFL RFFKDNLLKN GETES
//

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