(data stored in ACNUC7421 zone)

SWISSPROT: HPPK_BACSU

ID   HPPK_BACSU              Reviewed;         167 AA.
AC   P29252;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   11-DEC-2019, entry version 121.
DE   RecName: Full=2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase;
DE            EC=2.7.6.3;
DE   AltName: Full=6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase;
DE            Short=PPPK;
DE   AltName: Full=7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase;
DE            Short=HPPK;
GN   Name=folK; OrderedLocusNames=BSU00790;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT   chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-162.
RC   STRAIN=ASB342;
RX   PubMed=2123867; DOI=10.1128/jb.172.12.7211-7226.1990;
RA   Slock J., Stahly D.P., Han C.-Y., Six E.W., Crawford I.P.;
RT   "An apparent Bacillus subtilis folic acid biosynthetic operon containing
RT   pab, an amphibolic trpG gene, a third gene required for synthesis of para-
RT   aminobenzoic acid, and the dihydropteroate synthase gene.";
RL   J. Bacteriol. 172:7211-7226(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-
CC         6-yl)methyl diphosphate + AMP + H(+); Xref=Rhea:RHEA:11412,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:44841,
CC         ChEBI:CHEBI:72950, ChEBI:CHEBI:456215; EC=2.7.6.3;
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-
CC       4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-
CC       dihydroneopterin triphosphate: step 4/4.
CC   -!- SIMILARITY: Belongs to the HPPK family. {ECO:0000305}.
DR   EMBL; D26185; BAA05314.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11855.1; -; Genomic_DNA.
DR   EMBL; M34053; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S66109; S66109.
DR   RefSeq; NP_387960.1; NC_000964.3.
DR   RefSeq; WP_003242690.1; NZ_JNCM01000028.1.
DR   SMR; P29252; -.
DR   STRING; 224308.BSU00790; -.
DR   PaxDb; P29252; -.
DR   PRIDE; P29252; -.
DR   EnsemblBacteria; CAB11855; CAB11855; BSU00790.
DR   GeneID; 936940; -.
DR   KEGG; bsu:BSU00790; -.
DR   PATRIC; fig|224308.179.peg.79; -.
DR   eggNOG; ENOG4105K8U; Bacteria.
DR   eggNOG; COG0801; LUCA.
DR   HOGENOM; HOG000217741; -.
DR   InParanoid; P29252; -.
DR   KO; K00950; -.
DR   OMA; QVILRQN; -.
DR   PhylomeDB; P29252; -.
DR   BioCyc; BSUB:BSU00790-MONOMER; -.
DR   UniPathway; UPA00077; UER00155.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003848; F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046656; P:folic acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00483; HPPK; 1.
DR   Gene3D; 3.30.70.560; -; 1.
DR   InterPro; IPR000550; Hppk.
DR   InterPro; IPR035907; Hppk_sf.
DR   Pfam; PF01288; HPPK; 1.
DR   SUPFAM; SSF55083; SSF55083; 1.
DR   TIGRFAMs; TIGR01498; folK; 1.
DR   PROSITE; PS00794; HPPK; 1.
PE   3: Inferred from homology;
DR   PRODOM; P29252.
DR   SWISS-2DPAGE; P29252.
KW   ATP-binding; Folate biosynthesis; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..167
FT                   /note="2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
FT                   pyrophosphokinase"
FT                   /id="PRO_0000168247"
SQ   SEQUENCE   167 AA;  19058 MW;  BED6AA62C85E01E8 CRC64;
     MNNIAYIALG SNIGDRETYL RQAVALLHQH AAVTVTKVSS IYETDPVGYE DQAQFLNMAV
     EIKTSLNPFE LLELTQQIEN ELGRTREVRW GPRTADLDIL LFNRENIETE QLIVPHPRMY
     ERLFVLAPLA EICQQVEKEA TSAETDQEGV RVWKQKSGVD EFVHSES
//

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