(data stored in ACNUC7421 zone)

SWISSPROT: YACO_BACSU

ID   YACO_BACSU              Reviewed;         249 AA.
AC   Q06753;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   11-DEC-2019, entry version 116.
DE   RecName: Full=Putative TrmH family tRNA/rRNA methyltransferase YacO;
DE            EC=2.1.1.-;
GN   Name=yacO; OrderedLocusNames=BSU00960;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT   chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-143.
RX   PubMed=7510287;
RA   Gagnon Y., Breton R., Putzer H., Pelchat M., Grunberg-Manago M.,
RA   Lapointe J.;
RT   "Clustering and co-transcription of the Bacillus subtilis genes encoding
RT   the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and
RT   the first enzyme for cysteine biosynthesis.";
RL   J. Biol. Chem. 269:7473-7482(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
RX   PubMed=3145906; DOI=10.1016/0378-1119(88)90207-7;
RA   Smith H., de Jong A., Bron S., Venema G.;
RT   "Characterization of signal-sequence-coding regions selected from the
RT   Bacillus subtilis chromosome.";
RL   Gene 70:351-361(1988).
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase TrmH family. {ECO:0000305}.
DR   EMBL; D26185; BAA05329.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11872.1; -; Genomic_DNA.
DR   EMBL; L14580; AAA21799.1; -; Genomic_DNA.
DR   EMBL; M22906; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S66124; S66124.
DR   RefSeq; NP_387977.1; NC_000964.3.
DR   RefSeq; WP_004399684.1; NZ_JNCM01000029.1.
DR   SMR; Q06753; -.
DR   STRING; 224308.BSU00960; -.
DR   PaxDb; Q06753; -.
DR   PRIDE; Q06753; -.
DR   EnsemblBacteria; CAB11872; CAB11872; BSU00960.
DR   GeneID; 936861; -.
DR   KEGG; bsu:BSU00960; -.
DR   PATRIC; fig|224308.179.peg.99; -.
DR   eggNOG; ENOG4105C2N; Bacteria.
DR   eggNOG; COG0566; LUCA.
DR   HOGENOM; HOG000218798; -.
DR   InParanoid; Q06753; -.
DR   KO; K03218; -.
DR   OMA; QVPPYEY; -.
DR   PhylomeDB; Q06753; -.
DR   BioCyc; BSUB:BSU00960-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0070039; F:rRNA (guanosine-2'-O-)-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0000453; P:enzyme-directed rRNA 2'-O-methylation; IBA:GO_Central.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004441; rRNA_MeTrfase_TrmH.
DR   InterPro; IPR001537; SpoU_MeTrfase.
DR   InterPro; IPR013123; SpoU_subst-bd.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   Pfam; PF00588; SpoU_methylase; 1.
DR   Pfam; PF08032; SpoU_sub_bind; 1.
DR   SMART; SM00967; SpoU_sub_bind; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00186; rRNA_methyl_3; 1.
PE   3: Inferred from homology;
DR   PRODOM; Q06753.
DR   SWISS-2DPAGE; Q06753.
KW   Methyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..249
FT                   /note="Putative TrmH family tRNA/rRNA methyltransferase
FT                   YacO"
FT                   /id="PRO_0000159820"
FT   BINDING         198
FT                   /note="S-adenosyl-L-methionine; via carbonyl oxygen"
FT                   /evidence="ECO:0000250"
FT   BINDING         218
FT                   /note="S-adenosyl-L-methionine; via amide nitrogen and
FT                   carbonyl oxygen"
FT                   /evidence="ECO:0000250"
FT   BINDING         227
FT                   /note="S-adenosyl-L-methionine; via amide nitrogen"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   249 AA;  27509 MW;  A4135FF667974ABB CRC64;
     MSQQHDYVIG KNAVIETLKS DRKLYKLWMA ENTVKGQAQQ VIELAKKQGI TIQYVPRKKL
     DQMVTGQHQG VVAQVAAYEY AELDDLYKAA EEKNEQPFFL ILDELEDPHN LGSIMRTADA
     VGAHGIVIPK RRAVGLTTTV AKASTGAIEH IPVARVTNLA RTLEEMKERG IWVVGTDASA
     REDFRNMDGN MPLALVIGSE GKGMGRLVKE KCDFLIKLPM AGKVTSLNAS VAAGLLMYEV
     YRKRNPVGE
//

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