(data stored in ACNUC7421 zone)

SWISSPROT: RPSH_BACSU

ID   RPSH_BACSU              Reviewed;         218 AA.
AC   P17869;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   11-DEC-2019, entry version 142.
DE   RecName: Full=RNA polymerase sigma-H factor;
DE   AltName: Full=Sigma-30;
DE   AltName: Full=Stage 0 sporulation protein H;
GN   Name=sigH; Synonyms=spo0H; OrderedLocusNames=BSU00980;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3277943; DOI=10.1128/jb.170.3.1054-1062.1988;
RA   Dubnau E., Weir J., Nair G., Carter L. III, Moran C.P. Jr., Smith I.;
RT   "Bacillus sporulation gene spo0H codes for sigma 30 (sigma H).";
RL   J. Bacteriol. 170:1054-1062(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-218.
RX   PubMed=7968510; DOI=10.1111/j.1365-2958.1993.tb00910.x;
RA   Jeong S., Yoshikawa H., Takahashi H.;
RT   "Isolation and characterization of the secE homologue gene of Bacillus
RT   subtilis.";
RL   Mol. Microbiol. 10:133-142(1993).
RN   [4]
RP   MUTAGENESIS OF THR-100.
RC   STRAIN=168 / JH642;
RX   PubMed=2500529; DOI=10.1016/0022-2836(89)90569-x;
RA   Zuber P., Healy J., Carter H.L. III, Cutting S., Moran C.P. Jr., Losick R.;
RT   "Mutation changing the specificity of an RNA polymerase sigma factor.";
RL   J. Mol. Biol. 206:605-614(1989).
RN   [5]
RP   MUTAGENESIS OF ARG-96 AND THR-100.
RC   STRAIN=168 / JH642;
RX   PubMed=2122453; DOI=10.1073/pnas.87.20.8075;
RA   Daniels D., Zuber P., Losick R.;
RT   "Two amino acids in an RNA polymerase sigma factor involved in the
RT   recognition of adjacent base pairs in the -10 region of a cognate
RT   promoter.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:8075-8079(1990).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in the transition to post-
CC       exponential phase in the beginning of sporulation. It is also required
CC       for transcription of several stationary phase genes.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
DR   EMBL; M29693; AAA22756.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11874.1; -; Genomic_DNA.
DR   EMBL; D13303; BAA02557.1; -; Genomic_DNA.
DR   PIR; A28625; A28625.
DR   RefSeq; NP_387979.1; NC_000964.3.
DR   RefSeq; WP_009966312.1; NZ_JNCM01000029.1.
DR   SMR; P17869; -.
DR   STRING; 224308.BSU00980; -.
DR   PaxDb; P17869; -.
DR   PRIDE; P17869; -.
DR   DNASU; 936150; -.
DR   EnsemblBacteria; CAB11874; CAB11874; BSU00980.
DR   GeneID; 936150; -.
DR   KEGG; bsu:BSU00980; -.
DR   PATRIC; fig|224308.179.peg.101; -.
DR   eggNOG; COG1595; LUCA.
DR   HOGENOM; HOG000219029; -.
DR   InParanoid; P17869; -.
DR   KO; K03091; -.
DR   OMA; PIYDEDS; -.
DR   PhylomeDB; P17869; -.
DR   BioCyc; BSUB:BSU00980-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014218; RNA_pol_sigma-H.
DR   InterPro; IPR016371; RNA_pol_sigma-H_factor.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF08281; Sigma70_r4_2; 1.
DR   PIRSF; PIRSF002939; RNA_polymerase_sigma-H_factor; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   TIGRFAMs; TIGR02859; spore_sigH; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
PE   1: Evidence at protein level;
DR   PRODOM; P17869.
DR   SWISS-2DPAGE; P17869.
KW   DNA-binding; Reference proteome; Sigma factor; Sporulation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..218
FT                   /note="RNA polymerase sigma-H factor"
FT                   /id="PRO_0000093949"
FT   DNA_BIND        182..201
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           62..75
FT                   /note="Polymerase core binding"
FT   MUTAGEN         96
FT                   /note="R->A: Suppressor of mutations in the -12 region of
FT                   the spoVG promoter."
FT                   /evidence="ECO:0000269|PubMed:2122453"
FT   MUTAGEN         100
FT                   /note="T->A: Suppressor of mutations in the -13 region of
FT                   the spoVG promoter."
FT                   /evidence="ECO:0000269|PubMed:2122453,
FT                   ECO:0000269|PubMed:2500529"
FT   MUTAGEN         100
FT                   /note="T->I: Loss of ability to recognize wild-type spoVG
FT                   promoter. Suppressor of the spoVG249 mutation in the -10
FT                   region of the spoVG promoter."
FT                   /evidence="ECO:0000269|PubMed:2122453,
FT                   ECO:0000269|PubMed:2500529"
SQ   SEQUENCE   218 AA;  25447 MW;  7310E3FF153D99C1 CRC64;
     MNLQNNKGKF NKEQFCQLED EQVIEKVHVG DSDALDYLIT KYRNFVRAKA RSYFLIGADR
     EDIVQEGMIG LYKSIRDFKE DKLTSFKAFA ELCITRQIIT AIKTATRQKH IPLNSYASLD
     KPIFDEESDR TLLDVISGAK TLNPEEMIIN QEEFDDIEMK MGELLSDLER KVLVLYLDGR
     SYQEISDELN RHVKSIDNAL QRVKRKLEKY LEIREISL
//

If you have problems or comments...

PBIL Back to PBIL home page